Guide To Flow Cytometry Methods
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Guide To Flow Cytometry Methods
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Author : W. McLean Grogan
language : en
Publisher: CRC Press
Release Date : 1990-07-27
Guide To Flow Cytometry Methods written by W. McLean Grogan and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990-07-27 with Science categories.
Discusses the methodology and procedures used in studies of the cell cycle, cell development and differentiation, ageing, immunology, membrane fluidity, and aneuploidy analysis of the 15 most common forms of cancer. Described techniques of analysis include preparation of single-cell suspensions, DNA
Flow Cytometry
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Author :
language : en
Publisher: Elsevier
Release Date : 1994-12-12
Flow Cytometry written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994-12-12 with Science categories.
Flow Cytometry, Second Edition provides a complete and comprehensive two volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who must use flow cytometry to separate and analyze cells. Key Features * Completely revised and greatly expanded since the publication of the First Edition in 1990 * Methods cover cell death and cell cycle analyses Practical, handbook-style presentation works in lab or classroom * Unique comprehensive methodological coverage * Color plates illustrate techniques * In-depth treatment of procedures, including a description of each procedure: * Theoretical foundations * Critical aspects * Possible pitfalls * Written by authors with extensive experience who: * Developed or modified the techniques * Describe their experience with different instruments and applications to different cell systems * Are the Who's Who in Flow Cytometry
Flow Cytometry Protocols
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Author : Teresa S. Hawley
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Flow Cytometry Protocols written by Teresa S. Hawley and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
Flow cytometry has evolved since the 1940s into a multidisciplinary field incorporating aspects of laser technology, fluid dynamics, electronics, optics, computer science, physics, chemistry, biology, and mathematics. Innovations in instrumentation, development of small lasers, discovery of new fluorochromes/fluorescent proteins, and implementation of novel methodologies have all contributed to the recent rapid expansion of flow cytometry applications. In this thoroughly revised and updated second edition of Flow Cytometry Protocols, time-proven as well as cutting-edge methods are clearly and comprehensively presented by leading experimentalists. In addition to being a valuable reference manual for experienced flow cytometrists, the editors expect this authoritative up-to-date collection to prove useful to investigators in all areas of the biological and biomedical sciences who are new to the subject. The introductory chapter provides an eloquent synopsis of the principles and diverse uses of flow cytometry, beginning with a historical perspective and ending with a view to the future. Chapters 2–22 contain step-by-step protocols of highly practical and state-of-the-art techniques. Detailed instructions and helpful tips on experimental design, as well as selection of reagents and data analysis tools, will allow researchers to readily carry out flow cytometric investigations ranging from traditional phenotypic characterizations to emerging genomics and proteomics applications. Complementing these instructive protocols is a chapter that provides a preview of the next generation of solid-state lasers, and one that describes a rapid means to validate containment of infectious aerosols generated during high-speed sorting (Chapters 23–24).
Flow Cytometry
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Author : Zbigniew Darzynkiewicz
language : en
Publisher:
Release Date : 1994
Flow Cytometry written by Zbigniew Darzynkiewicz and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Medical categories.
Flow Cytometry, Second Edition provides a complete and comprehensive two volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who must use flow cytometry to separate and analyze cells. Key Features * Completely revised and greatly expanded since the publication of the First Edition in 1990 * Methods cover cell death and cell cycle analyses Practical, handbook-style presentation works in lab or classroom * Unique comprehensive methodological coverage * Color plates illustrate techniques * In-depth treatment of procedures, including a description of each procedure: * Theoretical foundations * Critical aspects * Possible pitfalls * Written by authors with extensive experience who: * Developed or modified the techniques * Describe their experience with different instruments and applications to different cell systems * Are the "Who's Who" in Flow Cytometry
Flow Cytometry Part B
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Author :
language : en
Publisher: Elsevier
Release Date : 1994-12-23
Flow Cytometry Part B written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994-12-23 with Science categories.
From the Reviews of the First Edition: "This is a good reference manual for multi-user facility faced with a wide variety of biological applications." - CYTOMETRY "Flow Cytometry includes an impressive array of methods applicable to chromosome analysis, plant biology, marine biology, fluorescence, insitu hybridization, and others. It succeeds in providing the reader with good insight into the power of the technology throughout biology." - KENNETH A. AULT, MAINE CYTOMETRY RESEARCH INSTITUTE, MAINE MEDICAL CENTER, IN CANCER CELLS Flow Cytometry, Second Edition provides a complete and comprehensive two-volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who use flow Cytometry to separate and analyze cells. * * Comprehensive methodological coverage in unique style * In depth treatment of procedures * Description of each procedure's: * Theoretical foundations * Critical aspects * Possible pitfalls * Written by authors with extensive experience who: * Developed or modified the technique * Describe their experience with different instruments and applications to different cell systems * Are the Who's Who in Flow Cytometry * 10 methods cover assessment of apoptosis and other modes of cell death * Practical, handbook-style presentation works in lab or classroom * Printed on acid-free paper * Color plates
Flow Cytometry
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Author : Albert D. Donnenberg
language : en
Publisher:
Release Date : 2017
Flow Cytometry written by Albert D. Donnenberg and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with SCIENCE categories.
"Flow cytometry is one of the most powerful analytics and preparative techniques in cell biology. This book details six of the most useful topics in flow cytometry derived from six lectures from recognized experts in the field. Each lecture is accompanied by expert commentary, all of which is written in an easy to read style. The cutting-edge chapters cover a wide array of topics including designing a flow cytometry experiment, rare event analysis, functional assays, assays of cell proliferation, solid tissues, and imaging flow cytometry. Each topic is carefully discussed for its current relevance in the field, its importance in biomedical research, and the frequency of its misuse in the scientific literature. "--Provided by publisher.
Methods In Molecular Biology
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Author :
language : en
Publisher:
Release Date : 2004
Methods In Molecular Biology written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Flow cytometry categories.
Handbook Of Flow Cytometry Methods
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Author : J. Paul Robinson
language : en
Publisher: Wiley-Liss
Release Date : 1993-03-25
Handbook Of Flow Cytometry Methods written by J. Paul Robinson and has been published by Wiley-Liss this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993-03-25 with Medical categories.
Handbook of Flow Cytometry Methods Edited By J. Paul Robinson Associate Editors: Zbigniew Darzynkiewicz, Phillip Dean, Lynn Dressler, Hans Tanke and Leon Wheeless Here is the ideal laboratory manual for investigators involved in research and diagnostic applications of flow cytometry. Handbook of flow cytometry methods offers a collection of proven, basic procedures designed for use in the flow cytometry laboratory. Methods implemented at Purdue University Cytometry Laboratories and developed in laboratories of contributors worldwide provide practical, step-by-step procedures for: the preparation and handling of standards,reagents and specimens laboratory analyses for immunophenotyping measurement of DNA content and cell cycle analyses determination of cell physiology and function set up and operation of flow cytometers computational and data analysis techniques safety precautions Drawing on their collective experience at leading research centers, the editors have fashioned a unique "working manual" intended to serve as the starting point for the evaluation and establishment of laboratory benchmark standards in flow and image cytometry. Each method is described in detail to be used and modified depending on the user’s needs. Special features of Handbook of Flow Cytometry Methods include: ample space throughout for personal annotations suggestions of alternative procedures; trouble-shooting tips extensive list of reagents and suppliers comb-bound format for ease of use at the lab bench Handbook of Flow Cytometry Methods is an essential laboratory resource for students, faculty, and researchers in cell and molecular biology, immunology, cancer biology, hematology/oncology, and laboratory medicine.
Essential Cytometry Methods
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Author : Zbigniew Darzynkiewicz
language : en
Publisher: Academic Press
Release Date : 2009-10-06
Essential Cytometry Methods written by Zbigniew Darzynkiewicz and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-10-06 with Science categories.
Cytometry is characterization and measurement of cells and cellular constituents, most often used to immunophenotype cells - that is, to distinguish healthy cells from diseased cells. Flow Cytometry specifically is quite sensitive, allowing researchers to detect rare cell types and residual levels of disease, and as such has been the method of choice for important studies such as monitoring the blood of AIDS patients. For this reason, there is a great need for a practical, comprehensive manual that will be useful across a broad range of laboratories. This volume, as part of the Reliable Lab Solution Series, delivers such a tool, offering busy researchers across many disciplines a handy resource of all the best methods and protocols for Cytometry to use at the bench. * Highlights top downloaded and cited chapters, authored by pioneers in the field and enhanced with their tips, and pitfalls to avoid. * Loaded with detailed protocols developed and used by leaders in the field. *Refines, organizes and updates popular methods from one of our top selling series, Methods in Cell Biology
Flow Cytometry
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Author : M. G. Ormerod
language : en
Publisher: Oxford University Press, USA
Release Date : 1990
Flow Cytometry written by M. G. Ormerod and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990 with Language Arts & Disciplines categories.
A laboratory handbook explaining the use of a specialized microscope with a flow chamber through which such entities as cells, nuclei, or chromosomes can be directed and counted. Designed to aid the newcomer to the field or the flow cytometrist wanting to try a new application. Distributed by Oxford University Press. Annotation copyrighted by Book News, Inc., Portland, OR