Essential Cytometry Methods
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Essential Cytometry Methods
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Author : Zbigniew Darzynkiewicz
language : en
Publisher: Academic Press
Release Date : 2009-10-06
Essential Cytometry Methods written by Zbigniew Darzynkiewicz and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-10-06 with Science categories.
Cytometry is characterization and measurement of cells and cellular constituents, most often used to immunophenotype cells - that is, to distinguish healthy cells from diseased cells. Flow Cytometry specifically is quite sensitive, allowing researchers to detect rare cell types and residual levels of disease, and as such has been the method of choice for important studies such as monitoring the blood of AIDS patients. For this reason, there is a great need for a practical, comprehensive manual that will be useful across a broad range of laboratories. This volume, as part of the Reliable Lab Solution Series, delivers such a tool, offering busy researchers across many disciplines a handy resource of all the best methods and protocols for Cytometry to use at the bench. * Highlights top downloaded and cited chapters, authored by pioneers in the field and enhanced with their tips, and pitfalls to avoid. * Loaded with detailed protocols developed and used by leaders in the field. *Refines, organizes and updates popular methods from one of our top selling series, Methods in Cell Biology
Handbook Of Flow Cytometry Methods
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Author : J. Paul Robinson
language : en
Publisher: Wiley-Liss
Release Date : 1993-03-25
Handbook Of Flow Cytometry Methods written by J. Paul Robinson and has been published by Wiley-Liss this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993-03-25 with Medical categories.
Handbook of Flow Cytometry Methods Edited By J. Paul Robinson Associate Editors: Zbigniew Darzynkiewicz, Phillip Dean, Lynn Dressler, Hans Tanke and Leon Wheeless Here is the ideal laboratory manual for investigators involved in research and diagnostic applications of flow cytometry. Handbook of flow cytometry methods offers a collection of proven, basic procedures designed for use in the flow cytometry laboratory. Methods implemented at Purdue University Cytometry Laboratories and developed in laboratories of contributors worldwide provide practical, step-by-step procedures for: the preparation and handling of standards,reagents and specimens laboratory analyses for immunophenotyping measurement of DNA content and cell cycle analyses determination of cell physiology and function set up and operation of flow cytometers computational and data analysis techniques safety precautions Drawing on their collective experience at leading research centers, the editors have fashioned a unique "working manual" intended to serve as the starting point for the evaluation and establishment of laboratory benchmark standards in flow and image cytometry. Each method is described in detail to be used and modified depending on the user’s needs. Special features of Handbook of Flow Cytometry Methods include: ample space throughout for personal annotations suggestions of alternative procedures; trouble-shooting tips extensive list of reagents and suppliers comb-bound format for ease of use at the lab bench Handbook of Flow Cytometry Methods is an essential laboratory resource for students, faculty, and researchers in cell and molecular biology, immunology, cancer biology, hematology/oncology, and laboratory medicine.
Imaging Flow Cytometry
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Author : Natasha S. Barteneva
language : en
Publisher: Humana
Release Date : 2015-11-23
Imaging Flow Cytometry written by Natasha S. Barteneva and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-11-23 with Medical categories.
This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.
Flow Cytometry Protocols
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Author : Teresa S. Hawley
language : en
Publisher: Springer Nature
Release Date :
Flow Cytometry Protocols written by Teresa S. Hawley and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.
Cellular Diagnostics
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Author : Ulrich Sack
language : en
Publisher: Karger Medical and Scientific Publishers
Release Date : 2009-01-01
Cellular Diagnostics written by Ulrich Sack and has been published by Karger Medical and Scientific Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-01-01 with Medical categories.
This book is the updated English version of the 2006 German bestseller Zellulare Diagnostik, a comprehensive presentation of flow cytometry and its applications. While some techniques of immunophenotyping by flow cytometry already are routine procedures in the laboratory, new methods for the functional characterization of cells, the analysis of rare cells, and the diagnosis of complex materials have only begun to win wide recognition. New approaches such as slide-based cytometry will lead to an increase in the use of cytometric techniques. Multiparameter approaches will further improve analysis. The book provides a comprehensive and detailed compilation of all aspects of flow cytometry in research and the clinic. For newcomers it offers a thorough introduction, for advanced users, specific protocols and interpretation assistance.
Flow Cytometry
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Author :
language : en
Publisher: Elsevier
Release Date : 1994-12-12
Flow Cytometry written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994-12-12 with Science categories.
Flow Cytometry, Second Edition provides a complete and comprehensive two volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who must use flow cytometry to separate and analyze cells. Key Features * Completely revised and greatly expanded since the publication of the First Edition in 1990 * Methods cover cell death and cell cycle analyses Practical, handbook-style presentation works in lab or classroom * Unique comprehensive methodological coverage * Color plates illustrate techniques * In-depth treatment of procedures, including a description of each procedure: * Theoretical foundations * Critical aspects * Possible pitfalls * Written by authors with extensive experience who: * Developed or modified the techniques * Describe their experience with different instruments and applications to different cell systems * Are the Who's Who in Flow Cytometry
Spectral And Imaging Cytometry
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Author : Natasha S. Barteneva
language : en
Publisher: Springer Nature
Release Date : 2023-04-19
Spectral And Imaging Cytometry written by Natasha S. Barteneva and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-04-19 with Science categories.
This second edition volume expands on the previous edition with discussions on the latest approaches used in the spectral cytometry field. Beginning with a brief history of spectral cytometry development, this book continues with a section addressing new protocols in different areas of imaging cytometry based on the unique technology of Imagestream and also introduces FlowCam – cytometer with capabilities of analyzing phytoplankton and zooplankton. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, complete lists of the materials and reagents necessary, and reproducible laboratory protocols with tips on troubleshooting and recommendations. Cutting-edge and thorough, Spectral and Imaging Flow Cytometry: Methods and Protocols, Second Edition is a valuable resources for beginners and professionals interested in learning more about this developing field. Chapters 1, 2, and 5 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Flow Cytometry
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Author : Zbigniew Darzynkiewicz
language : en
Publisher:
Release Date : 1994
Flow Cytometry written by Zbigniew Darzynkiewicz and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Medical categories.
Flow Cytometry, Second Edition provides a complete and comprehensive two volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who must use flow cytometry to separate and analyze cells. Key Features * Completely revised and greatly expanded since the publication of the First Edition in 1990 * Methods cover cell death and cell cycle analyses Practical, handbook-style presentation works in lab or classroom * Unique comprehensive methodological coverage * Color plates illustrate techniques * In-depth treatment of procedures, including a description of each procedure: * Theoretical foundations * Critical aspects * Possible pitfalls * Written by authors with extensive experience who: * Developed or modified the techniques * Describe their experience with different instruments and applications to different cell systems * Are the "Who's Who" in Flow Cytometry
Guide To Flow Cytometry Methods
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Author : W. McLean Grogan
language : en
Publisher: CRC Press
Release Date : 1990-07-27
Guide To Flow Cytometry Methods written by W. McLean Grogan and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990-07-27 with Science categories.
Discusses the methodology and procedures used in studies of the cell cycle, cell development and differentiation, ageing, immunology, membrane fluidity, and aneuploidy analysis of the 15 most common forms of cancer. Described techniques of analysis include preparation of single-cell suspensions, DNA
Mass Cytometry
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Author : Helen M. McGuire
language : en
Publisher: Humana
Release Date : 2019-05-13
Mass Cytometry written by Helen M. McGuire and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-05-13 with Science categories.
This book details up-to-date and cutting edge compilation of protocols in mass cytometry. Chapters guide readers through setting up a facility, panel design and reagent preparation, sample preparation, specific applications, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Cytometry: Methods and Protocols aims to ensure successful results in the further study of this vital field.