High Density Sequencing Applications In Microbial Molecular Genetics
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High Density Sequencing Applications In Microbial Molecular Genetics
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Author :
language : en
Publisher: Academic Press
Release Date : 2018-11-28
High Density Sequencing Applications In Microbial Molecular Genetics written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-11-28 with Science categories.
High-Density Sequencing Applications in Microbial Molecular Genetics, Volume 612 in the Methods of Enzymology series provides the latest on the high-density sequencing of DNA and cDNA libraries and how they have revolutionized contemporary research in biology. Methods permitting tens of millions of sequence reads in a single experiment have paved the way to genome-wide studies that are contributing to our understanding of the complexity of living systems. Chapters in this updated volume include Characterizing the role of exoribonucleases in the control of microbial gene expression: Differential RNA seq., Conformational studies of bacterial chromosomes by high-throughput sequencing methods, Measuring mRNA degradation, and more. Addition sections cover Global recognition patterns of bacterial RNA-binding proteins, High-resolution profiling of NMD targets, and the Generation of a metagenomic 3C/Hi-C library of human gut microbiota, Genome-wide mapping of yeast retrotransposons integration target sites, Measuring protein synthesis rates, Finding unsuspected partners of small RNAs with new screening approaches, Use of multiplexed transcriptomics to define the relationship between promoter sequence and transcription output, RNA-based control of quorum sensing in Vibrio cholerae, amongst other highly regarded topics. Detail methods used in research articles that were recently published in leading journals Provides the latest on the high-density sequencing of DNA and cDNA libraries and how they have revolutionized contemporary research in biology
High Throughput Next Generation Sequencing
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Author : Young Min Kwon
language : en
Publisher: Humana Press
Release Date : 2011-08-25
High Throughput Next Generation Sequencing written by Young Min Kwon and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-08-25 with Medical categories.
Due to their novel concepts and extraordinary high-throughput sequencing capacity, the “next generation sequencing” methods allow scientists to grasp system-wide landscapes of the complex molecular events taking place in various biological systems, including microorganisms and microbial communities. These methods are now being recognized as essential tools for a more comprehensive and deeper understanding of the mechanisms underlying many biological processes. In High-Throughput Next Generation Sequencing: Methods and Applications, experts in the field explore the most recent advances in the applications of next generation sequencing technologies with an emphasis on microorganisms and their communities; however, the methods described in this book will also offer general applications relevant to the study of any living organisms. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, High-Throughput Next Generation Sequencing: Methods and Applications is an excellent collection of chapters to aid all scientists who wish to apply these innovative research tools to enhance their own pursuits in microbiology and also biology in general.
Combining Simulations Theory And Experiments Into Multiscale Models Of Biological Events
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Author : Fabio Trovato
language : en
Publisher: Frontiers Media SA
Release Date : 2022-01-11
Combining Simulations Theory And Experiments Into Multiscale Models Of Biological Events written by Fabio Trovato and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-01-11 with Science categories.
Fundamentals Of Advanced Omics Technologies From Genes To Metabolites
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Author :
language : en
Publisher: Newnes
Release Date : 2014-02-14
Fundamentals Of Advanced Omics Technologies From Genes To Metabolites written by and has been published by Newnes this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-02-14 with Science categories.
Fundamentals of Advanced Omics Technologies: From Genes to Metabolites covers the fundamental aspects of the new instrumental and methodological developments in omics technologies, including those related to genomics, transcriptomics, epigenetics, proteomics and metabolomics, as well as other omics approaches such as glycomics, peptidomics and foodomics. The principal applications are presented in the following complementary volume. The chapters discuss in detail omics technologies, DNA microarray analysis, next-generation sequencing technologies, genome-wide analysis of methylation and histone modifications, emerging nanotechniques in proteomics, imaging mass spectrometry in proteomics, recent quantitative proteomics approaches, and advances in high-resolution NMR-based metabolomics, as well as MS-based non-targeted metabolomics and metabolome analysis by CE-MS, global glycomics analyses, foodomics, and high resolution analytical tools for quantitative peptidomics. Key aspects related to chemometrics, bioinformatics, data treatment, data integration and systems biology, deep-sequencing data analysis, statistical approaches for the analysis of microarray data, the integration of transcriptome and metabolome data and computational approaches for visualization and integration of omics data are also covered. Covers the latest advances in instrumentation, experimental design, sample preparation, and data analysis Provides thorough explanations and descriptions of specific omics technologies Describes advanced tools and methodologies for data pretreatment, storage, curation and analysis, as well as data integration
Microbiomes Of The Built Environment
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Author : National Academies of Sciences, Engineering, and Medicine
language : en
Publisher: National Academies Press
Release Date : 2017-10-06
Microbiomes Of The Built Environment written by National Academies of Sciences, Engineering, and Medicine and has been published by National Academies Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-10-06 with Science categories.
People's desire to understand the environments in which they live is a natural one. People spend most of their time in spaces and structures designed, built, and managed by humans, and it is estimated that people in developed countries now spend 90 percent of their lives indoors. As people move from homes to workplaces, traveling in cars and on transit systems, microorganisms are continually with and around them. The human-associated microbes that are shed, along with the human behaviors that affect their transport and removal, make significant contributions to the diversity of the indoor microbiome. The characteristics of "healthy" indoor environments cannot yet be defined, nor do microbial, clinical, and building researchers yet understand how to modify features of indoor environmentsâ€"such as building ventilation systems and the chemistry of building materialsâ€"in ways that would have predictable impacts on microbial communities to promote health and prevent disease. The factors that affect the environments within buildings, the ways in which building characteristics influence the composition and function of indoor microbial communities, and the ways in which these microbial communities relate to human health and well-being are extraordinarily complex and can be explored only as a dynamic, interconnected ecosystem by engaging the fields of microbial biology and ecology, chemistry, building science, and human physiology. This report reviews what is known about the intersection of these disciplines, and how new tools may facilitate advances in understanding the ecosystem of built environments, indoor microbiomes, and effects on human health and well-being. It offers a research agenda to generate the information needed so that stakeholders with an interest in understanding the impacts of built environments will be able to make more informed decisions.
Bioinformatics And Functional Genomics
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Author : Jonathan Pevsner
language : en
Publisher: John Wiley & Sons
Release Date : 2005-03-04
Bioinformatics And Functional Genomics written by Jonathan Pevsner and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-03-04 with Science categories.
Wiley is proud to announce the publication of the first ever broad-based textbook introduction to Bioinformatics and Functional Genomics by a trained biologist, experienced researcher, and award-winning instructor. In this new text, author Jonathan Pevsner, winner of the 2001 Johns Hopkins University "Teacher of the Year" award, explains problem-solving using bioinformatic approaches using real examples such as breast cancer, HIV-1, and retinal-binding protein throughout. His book includes 375 figures and over 170 tables. Each chapter includes: Problems, discussion of Pitfalls, Boxes explaining key techniques and math/stats principles, Summary, Recommended Reading list, and URLs for freely available software. The text is suitable for professionals and students at every level, including those with little to no background in computer science.
Next Generation Sequencing
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Author : Jerzy Kulski
language : en
Publisher: BoD – Books on Demand
Release Date : 2016-01-14
Next Generation Sequencing written by Jerzy Kulski and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-01-14 with Medical categories.
Next generation sequencing (NGS) has surpassed the traditional Sanger sequencing method to become the main choice for large-scale, genome-wide sequencing studies with ultra-high-throughput production and a huge reduction in costs. The NGS technologies have had enormous impact on the studies of structural and functional genomics in all the life sciences. In this book, Next Generation Sequencing Advances, Applications and Challenges, the sixteen chapters written by experts cover various aspects of NGS including genomics, transcriptomics and methylomics, the sequencing platforms, and the bioinformatics challenges in processing and analysing huge amounts of sequencing data. Following an overview of the evolution of NGS in the brave new world of omics, the book examines the advances and challenges of NGS applications in basic and applied research on microorganisms, agricultural plants and humans. This book is of value to all who are interested in DNA sequencing and bioinformatics across all fields of the life sciences.
Practical Bioinformatics For Beginners From Raw Sequence Analysis To Machine Learning Applications
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Author : Lloyd Wai Yee Low
language : en
Publisher: World Scientific
Release Date : 2023-01-17
Practical Bioinformatics For Beginners From Raw Sequence Analysis To Machine Learning Applications written by Lloyd Wai Yee Low and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-01-17 with Science categories.
Next-Generation Sequencing (NGS) is increasingly common and has applications in various fields such as clinical diagnosis, animal and plant breeding, and conservation of species. This incredible tool has become cost-effective. However, it generates a deluge of sequence data that requires efficient analysis. The highly sought-after skills in computational and statistical analyses include machine learning and, are essential for successful research within a wide range of specializations, such as identifying causes of cancer, vaccine design, new antibiotics, drug development, personalized medicine, and increased crop yields in agriculture.This invaluable book provides step-by-step guides to complex topics that make it easy for readers to perform specific analyses, from raw sequenced data to answer important biological questions using machine learning methods. It is an excellent hands-on material for lecturers who conduct courses in bioinformatics and as reference material for professionals. The chapters are standalone recipes making them suitable for readers who wish to self-learn selected topics. Readers gain the essential skills necessary to work on sequenced data from NGS platforms; hence, making themselves more attractive to employers who need skilled bioinformaticians.
Carrion Ecology Evolution And Their Applications
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Author : M. Eric Benbow
language : en
Publisher: CRC Press
Release Date : 2015-08-18
Carrion Ecology Evolution And Their Applications written by M. Eric Benbow and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-08-18 with Science categories.
Shortlisted for the 2018 TWS Wildlife Publication Awards in the edited book category Decomposition and recycling of vertebrate remains have been understudied, hampered largely due to these processes being aesthetically challenging (e.g., smell and sight). Technological innovations have provided the means to explore new and historically understood natural systems to give us a plethora of new information. Carrion Ecology, Evolution, and Their Applications covers a broad spectrum of topics including the molecular mechanistic foundations that provide the basis for intra- and interspecific interactions related to population biology, community ecology, and how this manifests into habitat- and ecosystem-level importance. The book connects the science of carrion decomposition from genes to ecosystems in multidisciplinary synthesis of the science. This book brings together a team of global experts involved with measuring and understanding the process and effects of carrion ecology in nature, with special application in such applied fields as forensic entomology, habitat management, animal production (e.g., livestock and aquaculture), and human and environmental health. It fills a large literature gap in ecology, providing a synthesis and future directions important for studies of carrion decomposition that improve the general understanding of decomposition in ecosystems. The book fuses multiple disciplines into a single message explaining the importance of vertebrate carrion ecology in nature. Illustrates Carrion Decomposition in a 16-Page Color Insert with 40 Photos The authors illustrate how the study of carrion transcends the globe and expands systems of inquiry, broadening awareness of this important ecosystem process. Whether you are a student, academic, or professional, you will find this book insightful for the fields of molecular ecology, microbiology, entomology, forensics, population biology, community and ecosystem ecology, and human and environmental health.
The New Science Of Metagenomics
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Author : National Research Council
language : en
Publisher: National Academies Press
Release Date : 2007-06-24
The New Science Of Metagenomics written by National Research Council and has been published by National Academies Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-06-24 with Science categories.
Although we can't usually see them, microbes are essential for every part of human life-indeed all life on Earth. The emerging field of metagenomics offers a new way of exploring the microbial world that will transform modern microbiology and lead to practical applications in medicine, agriculture, alternative energy, environmental remediation, and many others areas. Metagenomics allows researchers to look at the genomes of all of the microbes in an environment at once, providing a "meta" view of the whole microbial community and the complex interactions within it. It's a quantum leap beyond traditional research techniques that rely on studying-one at a time-the few microbes that can be grown in the laboratory. At the request of the National Science Foundation, five Institutes of the National Institutes of Health, and the Department of Energy, the National Research Council organized a committee to address the current state of metagenomics and identify obstacles current researchers are facing in order to determine how to best support the field and encourage its success. The New Science of Metagenomics recommends the establishment of a "Global Metagenomics Initiative" comprising a small number of large-scale metagenomics projects as well as many medium- and small-scale projects to advance the technology and develop the standard practices needed to advance the field. The report also addresses database needs, methodological challenges, and the importance of interdisciplinary collaboration in supporting this new field.