Histochemistry Of Single Molecules
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Histochemistry Of Single Molecules
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Author : Carlo Pellicciari
language : en
Publisher: Springer Nature
Release Date : 2022-09-24
Histochemistry Of Single Molecules written by Carlo Pellicciari and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-09-24 with Science categories.
This volume details histochemical techniques for the detection of specific molecules or metabolic processes, both at light and electron microscopy. Chapters are divided into seven sections covering Vital histochemistry, Carbohydrate histochemistry, Protein histochemistry, Lipid histochemistry, Nuclear histochemistry, Plant histochemistry and Histochemistry for Nanoscience. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. The volume also contains three discursive chapters on Histochemistry in advanced cytometry, Lectins and Detection of molecules in plant cell walls by fluorescence microscopy. Authoritative and cutting-edge, Histochemistry of Single Molecules: Methods and Protocols, Second Edition aims to be a useful practical guide for researchers to help further their study in this field.
Single Molecule Analysis
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Author : Erwin J. G. Peterman
language : en
Publisher: Humana Press
Release Date : 2011-09-15
Single Molecule Analysis written by Erwin J. G. Peterman and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-09-15 with Science categories.
Life scientists believe that life is driven, directed, and shaped by biomolecules working on their own or in concert. It is only in the last few decades that technological breakthroughs in sensitive fluorescence microscopy and single-molecule manipulation techniques have made it possible to observe and manipulate single biomolecules and measure their individual properties. The methodologies presented in Single Molecule Techniques: Methods and Protocols are being applied more and more to the study of biologically relevant molecules, such as DNA, DNA-binding proteins, and motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. The aim of Single Molecule Techniques: Methods and Protocols is to provide a broad overview of single-molecule approaches applied to biomolecules on the basis of clear and concise protocols, including a solid introduction to the most widely used single-molecule techniques, such as optical tweezers, single-molecule fluorescence tools, atomic force microscopy, magnetic tweezers, and tethered particle motion. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Single Molecule Techniques: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and provides a broad and thorough overview of the exciting and still-emerging field of single-molecule biology.
Single Molecule Techniques
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Author : Paul R. Selvin
language : en
Publisher: CSHL Press
Release Date : 2008
Single Molecule Techniques written by Paul R. Selvin and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Atomic force microscopy categories.
Geared towards research scientists in structural and molecular biology, biochemistry, and biophysics, this manual will be useful to all who are interested in observing, manipulating and elucidating the molecular mechanisms and discrete properties of macromolecules.
Single Molecule Enzymology
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Author : Gregory I. Mashanov
language : en
Publisher: Humana Press
Release Date : 2011-08-04
Single Molecule Enzymology written by Gregory I. Mashanov and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-08-04 with Science categories.
The last fifteen years have witnessed the birth and maturation of many original methods and the development of protocols specific to single molecule measurements and their analysis, including techniques involving optical imaging, electron microscopy, optical and magnetic trapping, and developments in atomic force microscopy. In Single Molecule Enzymology: Methods and Protocols, experts in the field provide procedures which enable the extraction of detailed information about enzyme work cycles, their static and kinetic properties, and information about their location and activity within cells. The detailed volume offers practical advice on many aspects of single molecule enzymology and includes strategic overviews of interconnected methods involved in sample preparation, single molecule measurements, and data analysis. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Single Molecule Enzymology: Methods and Protocols is intended for use within the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail and can be used by researchers planning their first single molecule study or to aid more experienced researchers in further developing their existing studies.
Immunohistochemistry
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Author : Charles Streckfus
language : en
Publisher: BoD – Books on Demand
Release Date : 2020-03-04
Immunohistochemistry written by Charles Streckfus and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-03-04 with Medical categories.
Immunohistochemistry - The Ageless Biotechnology is a book that is ideal for undergraduate and graduate biomedical researchers, and medical and dental health professionals. It is a detailed text, which emphasizes the laboratory and clinical implications of immunohistochemistry. The text covers the advances of immunohistochemistry from its humble origins in the 1930s up to the new decade of 2020. The book also offers a review of the immunohistochemistry detection systems with emphasis on their principles, history, and their advantages. It also stipulates the limitations and delineates the factors that need to be considered for choosing an appropriate detection system for IHC applications. The book describes current laboratory techniques and new applications for the technology. As the reader will observe, the book provides new and useful information concerning the rapidly advancing field of immunohistochemistry.
Enzyme Histochemistry
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Author : Z. Lojda
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Enzyme Histochemistry written by Z. Lojda and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
During recent years enzyme histochemical reactions have increasingly been considered as important, the reason being that enzyme histo chemistry is now a well-established link between morphology and bio chemistry. The development of numerous new methods and in particular the improvement of existing techniques contributed to the expansion of enzyme histochemical reactions. Today, the use of these methods allows detailed insight into molecular processes of single cells and their constituents. The selection of a suitable method for enzyme histochemical investigations needs thorough knowledge and critical evaluation of the reactions de scribed for the histochemical demonstration of enzymes and introduced in laboratory practice. Often, it is difficult for scientists primarily concerned with the application of methods and for laboratory assistants to comment on the value of an enzyme histochemical reaction. Our book will serve as a guide in this respect. It contains the most important histochemical methods for the localization of enzymes, all of which were checked by the authors themselves. These methods were often modified and frequently used for numerous different investigations of healthy and diseased organs in basic research and in routine practice.
Understanding Light Microscopy
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Author : Jeremy Sanderson
language : en
Publisher: John Wiley & Sons
Release Date : 2019-03-04
Understanding Light Microscopy written by Jeremy Sanderson and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-03-04 with Science categories.
Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.
Single Molecules And Nanotechnology
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Author : Rudolf Rigler
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-12-07
Single Molecules And Nanotechnology written by Rudolf Rigler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-12-07 with Technology & Engineering categories.
This book focuses on recent advances in the rapidly evolving field of single molecule research. These advances are of importance for the investigation of biopolymers and cellular biochemical reactions, and are essential to the development of quantitative biology. Written by leading experts in the field, the articles cover a broad range of topics, including quantum photonics of organic dyes and inorganic nanoparticles and monitoring of single molecule (enzymatic) reactions.
Male Infertility
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Author : Sijo J. Parekattil
language : en
Publisher: Springer Nature
Release Date : 2020-01-24
Male Infertility written by Sijo J. Parekattil and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-01-24 with Health & Fitness categories.
A groundbreaking contribution to the literature now in its revised and expanded second edition, this textbook offers a comprehensive review of diagnostic and treatment techniques for male infertility. This state-of-the-art, evidence-based textbook incorporates new multidisciplinary and complementary medicine approaches to create a first-of-its-kind guide to treatment strategies for male infertility and beyond. While this new edition is primarily designed as a reference for students and residents in reproductive medicine and andrology, it will be equally useful as well for professionals in urology, reproductive endocrinology, embryology, and research fields who are interested in the role that antioxidants play in male infertility. World-renowned experts in these areas have been selected to participate in this work. Careful selection of the highest quality content will span the whole range of topics in the area of male infertility, providing a complete review of well-established and current diagnostic and treatment techniques for male infertility. The incorporation of 20 new chapters will enhance the book’s appeal by including the most recent advances brought to the male infertility arena. Additionally, this edition incorporates new features, including bulleted key points, review criteria and select video clips demonstrating some of the most fascinating male infertility treatment modalities. A dedicated new section on current guidelines on male infertility will enlighten readers on how to most optimally manage male infertility clinical scenarios. Covering all aspects of diagnosis and management, ART, lifestyle factors and associated conditions for male infertility, Male Infertility: Contemporary Clinical Approaches, Andrology, ART and Antioxidants will be a readily accessible, high quality reference for medical students and residents, and will be of significant value to professionals working in the various fields treating this condition as well.
Standard And Super Resolution Bioimaging Data Analysis
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Author : Ann Wheeler
language : en
Publisher: John Wiley & Sons
Release Date : 2017-12-26
Standard And Super Resolution Bioimaging Data Analysis written by Ann Wheeler and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-26 with Science categories.
A comprehensive guide to the art and science of bioimaging data acquisition, processing and analysis Standard and Super-Resolution Bioimaging Data Analysis gets newcomers to bioimage data analysis quickly up to speed on the mathematics, statistics, computing hardware and acquisition technologies required to correctly process and document data. The past quarter century has seen remarkable progress in the field of light microscopy for biomedical science, with new imaging technologies coming on the market at an almost annual basis. Most of the data generated by these systems is image-based, and there is a significant increase in the content and throughput of these imaging systems. This, in turn, has resulted in a shift in the literature on biomedical research from descriptive to highly-quantitative. Standard and Super-Resolution Bioimaging Data Analysis satisfies the demand among students and research scientists for introductory guides to the tools for parsing and processing image data. Extremely well illustrated and including numerous examples, it clearly and accessibly explains what image data is and how to process and document it, as well as the current resources and standards in the field. A comprehensive guide to the tools for parsing and processing image data and the resources and industry standards for the biological and biomedical sciences Takes a practical approach to image analysis to assist scientists in ensuring scientific data are robust and reliable Covers fundamental principles in such a way as to give beginners a sound scientific base upon which to build Ideally suited for advanced students having only limited knowledge of the mathematics, statistics and computing required for image data analysis An entry-level text written for students and practitioners in the bioscience community, Standard and Super-Resolution Bioimaging Data Analysis de-mythologises the vast array of image analysis modalities which have come online over the past decade while schooling beginners in bioimaging principles, mathematics, technologies and standards.
