Hplc And Fplc Troubleshooting And Standardizing Chromatogram Purification Profiles
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Hplc And Fplc Troubleshooting And Standardizing Chromatogram Purification Profiles
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Author : T. S. Amar Anand Rao
language : en
Publisher: GRIN Verlag
Release Date : 2012
Hplc And Fplc Troubleshooting And Standardizing Chromatogram Purification Profiles written by T. S. Amar Anand Rao and has been published by GRIN Verlag this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with Medical categories.
Scientific Study from the year 2012 in the subject Biology - Micro- and Molecular Biology, Indian Institute of Science, course: Molecular Biology, language: English, abstract: This is a tool to separate compounds according to its hydrophobicity or hydrophilicity. A gradient is given from hydrophilic to hydrophobic range of solvent moving through a solid matrix. Here are some valuable tips collected through experience and handed over so that the reader can find it useful when the chromatograms and the instruments prove so cross with you. The instrumentation and working for both the systems HPLC and FPLC are almost same. HPLC is High Performance/Pressure Liquid Chromatography and FPLC is Fast Protein Liquid Chromtography.
Maintaining And Troubleshooting Hplc Systems
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Author : Dennis J. Runser
language : en
Publisher:
Release Date : 1981-05-06
Maintaining And Troubleshooting Hplc Systems written by Dennis J. Runser and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1981-05-06 with Reference categories.
Provides users of HPLC equipment with a comprehensive text for troubleshooting and maintaining HPLC systems. Describes how the chromatographer can maintain the HPLC system in operating condition, what to look for and do to prevent and solve HPLC problems, and what can and should be done before calling a service representative. Organized into chapters which basically represent the typical components of the HPLC system, with each chapter describing a basic element of the HPLC system in terms of maintenance and solving system problems. Arranged as a guide and working manual to help the chromatographer reduce instrument downtime, allowing for more efficiency and cost effectiveness in the HPLC laboratory.
Troubleshooting Hplc Systems
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Author : Paul C. Sadek
language : en
Publisher:
Release Date : 2002-02-01
Troubleshooting Hplc Systems written by Paul C. Sadek and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-02-01 with categories.
Approaches To The Purification Analysis And Characterization Of Antibody Based Therapeutics
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Author : Allan Matte
language : en
Publisher: Elsevier
Release Date : 2020-09-07
Approaches To The Purification Analysis And Characterization Of Antibody Based Therapeutics written by Allan Matte and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-09-07 with Business & Economics categories.
Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics provides the interested and informed reader with an overview of current approaches, strategies and considerations relating to the purification, analytics and characterization of therapeutic antibodies and related molecules. While there are obviously other books published in and around this subject area, they seem to be either older (c.a. year 2000 publication date) or are more limited in scope. The book will include an extensive bibliography of the published literature in the respective areas covered. It is not, however, intended to be a how-to methods book. Covers the vital new area of R&D on therapeutic antibodies Written by leading scientists and researchers Up-to-date coverage and includes a detailed bibliography
Biofilter For The Purification Of Air Contaminated With Triethylamine Tea
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Author : Venu Gopal
language : en
Publisher:
Release Date : 2015-01-29
Biofilter For The Purification Of Air Contaminated With Triethylamine Tea written by Venu Gopal and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-01-29 with categories.
Master's Thesis from the year 2012 in the subject Chemistry - Bio-chemistry, course: Master Degree, language: English, abstract: Modern air pollution control technologies have emerged over the past 40 years to mitigate emission problems in industrial facilities and thereby comply with environmental regulations. A variety of technologies have been developed to meet the needs of both the industries and the regulatory agencies keeping in view the techno economics. Some air pollutants like H2S, NH3 and VOC's are emitted in the industry causes odour and this not only causes occupational health but also damages public image of the company. Engineered biofiltration is a sustainable technology for VOC and odor control. Bio-filtration is a method of pollution control in which pollutants are biologically degraded using microorganisms. Generally, the energy demands for biofiltration are one-fourth to one-tenth that of physico-chemical destructive technologies. Biofilters are being developed and effectively used for a wide variety of industries, including wood products, paint manufacturing, petroleum remediation etc. Biofilters are cost-effective and straightforward options for pollutants capable of biodegrading reasonably easily. Triethylamine(TEA) is a Volatile organic compound widely used as a catalyst for polymerization reactions and a solvent and corrosion inhibitor in industry and it is also used as an intermediate in the production of various chemicals, including pesticides. It is necessary to remove TEA from water and gas in the environment. TEA gas-phase bio treatment has emerged as an effective and inexpensive alternative to conventional physicochemical treatment systems. The technology is still under development in terms of economics, equipment, process kinetics, and operational skills and different layouts and flow trains are being proposed including biofiltration, biotrickling filter, and bioscrubber. In the present work, studies are being carried out on biofilte
Development And Validation Of Hplc Method For Simultaneous Quantitative Determination Of Azilsartan Medoxomil Potassium And Chlorthalidone In Human Plasma
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Author : Vijay Ram
language : en
Publisher: GRIN Verlag
Release Date : 2014-12-17
Development And Validation Of Hplc Method For Simultaneous Quantitative Determination Of Azilsartan Medoxomil Potassium And Chlorthalidone In Human Plasma written by Vijay Ram and has been published by GRIN Verlag this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-12-17 with Science categories.
Doctoral Thesis / Dissertation from the year 2014 in the subject Chemistry - Analytical Chemistry, grade: 3, Saurashtra University (Department of Chemistry), course: Ph.D., language: English, abstract: The objective of this work was to develop a simple, cost effective, rugged and a high throughput method for simultaneous estimation of Azilsartan and Chlorthalidone in human plasma. Solid phase extraction technique is introduced here for first time and its advantages are: (I) Short processing time, (II) Significant reduction in the labour and (III) This technique minimizes chances of errors, saves considerable time and simplifies the sample preparation methodology. The run time per sample analysis of 15.0 min suggests the high throughput of the proposed method. From the results of all the validation parameters, the method proposed here can be useful for therapeutic drug monitoring both for analysis of routine samples of single dose or multiple dose pharmacokinetics and also for the clinical trial samples.
Textbook On Cloning Expression And Purification Of Recombinant Proteins
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Author : Kakoli Bose
language : en
Publisher: Springer Nature
Release Date : 2022-01-25
Textbook On Cloning Expression And Purification Of Recombinant Proteins written by Kakoli Bose and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-01-25 with Medical categories.
This book is immensely useful for graduate students as well as researchers to understand the basics of molecular biology and Recombinant DNA Technology. It provides a comprehensive overview of different approaches for the synthesis of recombinant proteins from E. coli including their cloning, expression and purification. Recent advances in genomics, proteomics, and bioinformatics have facilitated the use of Recombinant DNA Technology for evaluating the biophysical and biochemical properties of various proteins. The book starts with an introductory chapter on gene cloning, protein expression and purification and its implication in current research and commercial applications. Each chapter provides a lucid set of principles, tools and techniques for both students and instructors. The protocols described have been aptly exemplified, and troubleshooting techniques have been included to aid better understanding. Moreover, the set of questions at the end of each chapter have been particularly formulated to help effective learning.
Hplc And Uhplc For Practicing Scientists
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Author : Michael W. Dong
language : en
Publisher: John Wiley & Sons
Release Date : 2019-07-23
Hplc And Uhplc For Practicing Scientists written by Michael W. Dong and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-07-23 with Science categories.
A concise yet comprehensive reference guide on HPLC/UHPLC that focuses on its fundamentals, latest developments, and best practices in the pharmaceutical and biotechnology industries Written for practitioners by an expert practitioner, this new edition of HPLC and UHPLC for Practicing Scientists adds numerous updates to its coverage of high-performance liquid chromatography, including comprehensive information on UHPLC (ultra-high-pressure liquid chromatography) and the continuing migration of HPLC to UHPLC, the modern standard platform. In addition to introducing readers to HPLC’s fundamentals, applications, and developments, the book describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. HPLC and UHPLC for Practicing Scientists, Second Edition offers three new chapters. One is a standalone chapter on UHPLC, covering concepts, benefits, practices, and potential issues. Another examines liquid chromatography/mass spectrometry (LC/MS). The third reviews at the analysis of recombinant biologics, particularly monoclonal antibodies (mAbs), used as therapeutics. While all chapters are revised in the new edition, five chapters are essentially rewritten (HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects). The book also includes problem and answer sections at the end of each chapter. Overviews fundamentals of HPLC to UHPLC, including theories, columns, and instruments with an abundance of tables, figures, and key references Features brand new chapters on UHPLC, LC/MS, and analysis of recombinant biologics Presents updated information on the best practices in method development, validation, operation, troubleshooting, and maintaining regulatory compliance for both HPLC and UHPLC Contains major revisions to all chapters of the first edition and substantial rewrites of chapters on HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects Includes end-of-chapter quizzes as assessment and learning aids Offers a reference guide to graduate students and practicing scientists in pharmaceutical, biotechnology, and other industries Filled with intuitive explanations, case studies, and clear figures, HPLC and UHPLC for Practicing Scientists, Second Edition is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology. It will be a great benefit to every busy laboratory analyst and researcher.
Hplc Of Peptides And Proteins
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Author : Marie-Isabel Aguilar
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Hplc Of Peptides And Proteins written by Marie-Isabel Aguilar and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.
Affinity Chromatography
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Author : Pascal Bailon
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Affinity Chromatography written by Pascal Bailon and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
Affinity chromatography, with its exquisite specificity, is based upon molecular recognition. It is a powerful tool for the purification of biomolecules. In recent years, numerous new applications and modified techniques have been derived from gro- specific interactions and biological recognition principles. An up-to-date review of the past, current, and future applications of affinity chromatography has been presented in the introductory chapter by Meir Wilchek and Irwin Chaiken. Though many of these new applications and techniques are well documented in the literature, it is often difficult to find methods that are written with the intent of helping new practitioners of affinity chromatography. This volume on Affinity Chromatography: Methods and Protocols is intended for the novice, as well as for - perts in the field. The protocols are written by experts who have developed and/or successfully employed these methods in their laboratories. Each chapter describes a specific technique, and since the book is intended to help the beginner, each technique is described simply and clearly, making sure that all relevant steps are included, assuming no previous knowledge. Each chapter contains an introduction describing the principles involved, followed by a Materials and Methods section, which lays the groundwork for the reader to conduct experiments step-by-step, in an orderly fashion. The following Notes section, which describes many of the problems that can occur, makes suggestions for overcoming them, and provides alternate procedures. These are precisely the sort of important, practical details that never seem to appear in the published literature.