In Situ Hybridization In Neurobiology
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In Situ Hybridization
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Author : Karen L. Valentino
language : en
Publisher: Oxford University Press, USA
Release Date : 1987
In Situ Hybridization written by Karen L. Valentino and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987 with Language Arts & Disciplines categories.
A technique for reading the messenger RNAs by which cells express their distinctive populations of structural and functional proteins, in situ hybridization is now reliable and practical for use in neurobiological research.
In Situ Hybridization In Neurobiology
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Author : James H. Eberwine
language : en
Publisher:
Release Date : 1994
In Situ Hybridization In Neurobiology written by James H. Eberwine and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with In situ hybridization categories.
In situ hybridization has become an important and widely used research tool for neurobiologists since the authors' first book on the subject, In Situ Hybridization: Applications to Neurobiology (Oxford, 1987). This new volume examines the methodological advances that have been made in in situhybridization techniques and their impact on the study of the nervous system. These include methods for improving sensitivity, ways of examining multiple mRNAs within the same section, ways of examining both proteins and mRNAs within the same section, and methods for using in situ hybridizationtechnology to examine areas of cellular biological significance, such as translational control. The development of alternative labels to radioactivity for the detection of hybridized probes, and the combined use of in situ hybridization and immunocytochemistry is also covered in this book. Itdiscusses the quantification of in situ hybridization signal and presents an overview of information generated by using in situ hybridization in diagnostic medicine. This volume will be essential to researchers in the neurosciences as well as those in other disciplines.
In Situ Hybridization Protocols For The Brain
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Author : W. Wisden
language : en
Publisher: Gulf Professional Publishing
Release Date : 2002-08-05
In Situ Hybridization Protocols For The Brain written by W. Wisden and has been published by Gulf Professional Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-08-05 with Medical categories.
This volume of the International Review of Neurobiology was written to assist researchers without any previous experience with in situ hybridization, allowing them to follow the protocols and expect good results. It contains all the information required for newcomers to achieve successful in situ hybridization results, and methods for improving the technique of those already utilizing it. Published since 1959, International Review of Neurobiology is a well-known series appealing to neuroscientists, clinicians, psychologists, physiologists, and pharmacologists. Led by an internationally renowned editorial board, this important serial publishes both eclectic volumes made up of timely reviews and thematic volumes that focus on recent progress in a specific area of neurobiology research. A well-known series appealing to neuroscientists, clinicians, psychologists, physiologists, and pharmacologists Led by an internationally renowned editorial board, this important serial publishes both eclectic volumes made up of timely reviews and thematic volumes that focus on recent progress in a specific area of neurobiology research
In Situ Hybridization In Brain
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Author : George Uhl
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
In Situ Hybridization In Brain written by George Uhl and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The explosion of interest in specific molecules important for brain function and dysfunction has drawn individuals from diverse backgrounds toward the use of in situ hybridization techniques. Study of the brain demands the anatomic precision and biochemical specificity that this approach can potentially bring. Workers with backgrounds in peptide neuroanatomy, neuropharmacology, molecular biology, neurovirology, neuropathology, and neurophysiology have joined together in this volume to discuss their initial experiences in applying ill situ hybridization techniques to the study of the brain. The work, although still in an early phase of development, is worthy of initial summary and dissemination. In the area of neuropeptide gene expression alone, investigators represented here describe studies of vasopressin, opiate peptides, oxytocin, vasoactive intestinal peptide, cholecystokinin, and somatostatin. Other contributions provide insight into applications of the technique to studies of the expression of genes for neurotransmitter synthesizing enzymes, viral-encoded genes, trophic factor genes, and the genes selected on the basis of their special roles in the brain. The authors provide an important series of technical perspectives, and describe specific experimental protocols. This volume should be of interest to individuals seeking an introduction to these methods, as well to those desiring an up to date precis of work in this burgeoning area. Dr. Uhl, with the sponsorship of the Howard Hughes Medical Institute, has done a superb job of assembling the leaders in this area, and in organizing the presen ta tion of their perspecti ves herein. Joseph B. Martin, M.D., Ph.D.
In Situ Hybridization Techniques For The Brain
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Author : Z. Henderson
language : en
Publisher:
Release Date : 1996-12-11
In Situ Hybridization Techniques For The Brain written by Z. Henderson and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-12-11 with Medical categories.
Written by molecular biologists and neurobiologists, this volume in the Handbook Series provides basic step-by-step protocols in addition to specialist techniques to support those practising in situ hybridization, a powerful histochemical technique which is now widely used in neurobiology. The first part of the manual concentrates on basic procedures, eg, which probe and label to select, equipment, relevant molecular biological techniques, hazards, probe preparation and labelling, tissue preparation, and conditions for hybridization. Specialist procedures including quantitative analysis of data, reverse transcription PCR, working with human tissue, and double label methods are presented in subsequent sections. In Situ Hybridization Techniques for the Brain is, therefore, an essential reference source for all those with research interests in neuroanatomy, histopathology and/or neurohistochemistry, and will support users in both the preparation and successful completion of the procedures in their own laboratory.
In Situ Hybridization Methods
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Author : Giselbert Hauptmann
language : en
Publisher: Humana
Release Date : 2016-10-05
In Situ Hybridization Methods written by Giselbert Hauptmann and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-10-05 with Medical categories.
This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications. The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Essential and authoritative, In Situ Hybridization Methods provides detailed protocols for newcomers to ISH, and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications.
In Situ Hybridization
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Author : Ashley T. Haase
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
In Situ Hybridization written by Ashley T. Haase and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
Leading researchers present contemporary treatment of in situ hybridization applied to current issues in animal virus pathogenesis. The most recent methods are given for locating viral genes in whole animal section and for defining the number and type of cells surrounded by viruses. The genetic programs played out in these cells and the newer methods of hybridization at the electron microscopic level provide valuable insight into the complexities of virus-host interaction.
Molecular Imaging In Neuroscience
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Author : Najam Arfeen Sharif
language : en
Publisher: IRL Press
Release Date : 1993
Molecular Imaging In Neuroscience written by Najam Arfeen Sharif and has been published by IRL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with Autoradiography categories.
This book reflects the recent dramatic advances in the use of modern imaging techniques to visualize and quantify the various components of cell structure and cell functional activity in the nervous system. Readers will find detailed protocols for imaging neurotransmitter and receptor mRNAs using radioactive and non-radioactive in situ hybridization technology. Methods for visualizing transmitter and drug receptors using quantitative autoradiography, sequence-specific antibodies, and immunocytochemistry are also clearly described. Plus, the book covers the imaging of biological activity in living cells, including methods of monitoring intracellular pH and calcium mobilization using selective ion-sensitive dyes in conjunction with flow cytometry and video microscopy. This is an essential guide for researchers wanting to exploit these powerful new techniques for their studies in neuropharmacology, neuroanatomy, neurogenetics, neuropathology, and pharmaceutical research. receptors, and receptor function in the nervous system. They represent the state of the art for studying brain function and dysfunction in the laboratory. It will be of major interest to neuroscientists and pharmacologists.
Gene Expression In Neural Tissues
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Author : P. Michael Conn
language : en
Publisher:
Release Date : 1992
Gene Expression In Neural Tissues written by P. Michael Conn and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with Medical categories.
The study of gene expression in neural tissue brings to bear some of the newest and most productive scientific technology to one of the most exciting and fastest growing areas. This volume describes methods for the study of the expression of specific genes frequently found in very low abundance. Key Features * Convenient benchtop format * Methods presented for easy adaptation to new systems * Comprehensive protocols included for * In situ hybridization * Comparison of in situ and immunochemical methods for the study of regulatory molecules * Gene transfer * The study of the regulation of gene expression * The enhancer trap method * Quantifying rare mRNAs * Isolation and use of cell lines * The homology approach for receptor cloning * Trouble Shooting
Molecular Neurobiology
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Author : B. David Hames
language : en
Publisher: Oxford University Press, USA
Release Date : 1989
Molecular Neurobiology written by B. David Hames and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989 with Science categories.