In Situ Hybridization Protocols
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In Situ Hybridization Protocols
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Author : Ian A. Darby
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
In Situ Hybridization Protocols written by Ian A. Darby and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
The technique of in situ hybridization, in its various forms, has been used routinely in many laboratories for a number of years. In the post-genome era, gene arrays and proteomics have allowed us to identify hitherto unknown unrecognized pathways and mechanisms. However, rather than diminish the importance of in situ hybridization, the now widespread use of screening te- nologies has increased the need to temporally and spatially localize the dist- bution of mRNA expression. Our intention, in In Situ Hybridization Protocols is to provide ample inf- mation for novices planning to set up the in situ hybridization technique and use it in their laboratory for the first time, as well as giving updates of recent developments for those laboratories where in situ hybridization techniques are already in use. Despite its widespread significance, in situ hybridization has retained a re- tation as one of the more difficult and capricious molecular biological te- niques. This may in part be because of the hybrid nature of the technique, which often requires a mixture of molecular biological and histological skills. The two techniques are usually taught and acquired in different streams of biolo- cal science. The step-by-step and detailed protocols provided in In Situ Hybridization Protocols by researchers active in the field should make it p- sible for both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use the technique s- cessfully in their laboratories.
In Situ Hybridization Protocols For The Brain
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Author : W. Wisden
language : en
Publisher: Gulf Professional Publishing
Release Date : 2002-08-05
In Situ Hybridization Protocols For The Brain written by W. Wisden and has been published by Gulf Professional Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-08-05 with Medical categories.
This volume of the International Review of Neurobiology was written to assist researchers without any previous experience with in situ hybridization, allowing them to follow the protocols and expect good results. It contains all the information required for newcomers to achieve successful in situ hybridization results, and methods for improving the technique of those already utilizing it. Published since 1959, International Review of Neurobiology is a well-known series appealing to neuroscientists, clinicians, psychologists, physiologists, and pharmacologists. Led by an internationally renowned editorial board, this important serial publishes both eclectic volumes made up of timely reviews and thematic volumes that focus on recent progress in a specific area of neurobiology research. A well-known series appealing to neuroscientists, clinicians, psychologists, physiologists, and pharmacologists Led by an internationally renowned editorial board, this important serial publishes both eclectic volumes made up of timely reviews and thematic volumes that focus on recent progress in a specific area of neurobiology research
In Situ Hybridization Protocols
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Author : Boye Nielsen
language : en
Publisher: Humana Press
Release Date : 2014-09-13
In Situ Hybridization Protocols written by Boye Nielsen and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-09-13 with Medical categories.
In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, In Situ Hybridization Protocols, Fourth Edition seeks to aid scientists in the further discovery of new RNA species and uncovering of their cellular functions.
Fluorescence In Situ Hybridization Fish
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Author : Gal Haimovich
language : en
Publisher: Springer Nature
Release Date :
Fluorescence In Situ Hybridization Fish written by Gal Haimovich and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.
Pcr In Situ Hybridization
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Author : Gerard J. Nuovo
language : en
Publisher:
Release Date : 1997
Pcr In Situ Hybridization written by Gerard J. Nuovo and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Cytodiagnosis categories.
Fluorescence In Situ Hybridization Fish Application Guide
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Author : Thomas Liehr
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-11-26
Fluorescence In Situ Hybridization Fish Application Guide written by Thomas Liehr and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-11-26 with Science categories.
This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.
In Situ Hybridization Protocols
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Author : Ian A. Darby
language : en
Publisher: Taylor & Francis US
Release Date : 2000
In Situ Hybridization Protocols written by Ian A. Darby and has been published by Taylor & Francis US this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Medical categories.
Annotation Darby (human biology, RMIT U., Victoria, Australia) is joined by geneticists, molecular biologists, and pathologists from around the world to describe basic and advanced techniques for hybridization, for whole-mount embryo specimens and at the electron microscope level. Coverage includes protocols for detection of DNA fragmentation in apoptosis, localization of genes to particular chromosomes, and the use of DNA and RNA probes to detect expression in cells or tissue sections. For novice and experienced investigators who need proven and readily reproducible methods. Annotation c. Book News, Inc., Portland, OR (booknews.com)
In Situ Hybridization
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Author : David G. Wilkinson
language : en
Publisher: OUP Oxford
Release Date : 1998-11-26
In Situ Hybridization written by David G. Wilkinson and has been published by OUP Oxford this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-11-26 with Science categories.
In situ hybridization is used to reveal the location of specific nucleic acids sequences on chromosomes or in tissues. Visualization of the location of genes on chromosomes or of specific mRNAs or viruses in tissues is crucial for understanding the organization, regulation, and function of genes. It is a therefore a core technique in all areas of biomedical research. In Situ Hybridization: A Practical Approach 2/e is the second edition of one of the most successful Practical Approach books, published in 1992. Since the first edition was published, a number of important technical advances have been made. The new edition has been thoroughly updated to contain protocols detailing the major techniques of in situ hybridization currently in use: in situ hybridization to mRNA with oligonucleotide and RNA probes (radiolabelled and hapten labelled); analysis using light and electron microscopes; whole mount in situ hybridization; double detection of RNAs, and RNA plus protein; and fluorescent in situ hybridization to detect chromosomal sequences. The protocols are complemented by advice on strategies for successful results, descriptions of the theoretical basis of in situ hybridization and important new developments in gene expression databases. The procedures described are widely applicable to many systems. The use of in situ hybridization in PCR is covered in a separate volume: Herrington and O'Leary (Eds) PCR 3 - PCR in situ hybridization: A Practical Approach (OUP, 1997). All the authors have extensive practical experience of establishing reliable techniques of in situ hybridization. This book will be useful to all researchers at all levels who use in situ hybridization.
Fluorescence In Situ Hybridization Fish
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Author : Joanna M. Bridger
language : en
Publisher: Humana
Release Date : 2016-08-23
Fluorescence In Situ Hybridization Fish written by Joanna M. Bridger and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Science categories.
Fluorescence in situ Hybridization (FISH) belongs to that special category of well-established molecular biology techniques that, since their inception a few decades ago, have succeeded in keeping a prominent position within the constantly expanding list of laboratory pro- dures for biomedical research and clinical diagnostics. The design simplicity and cost-effectiveness of the early FISH protocols, combined with the signifcant acceleration of discoveries in related technical areas such as fuor- cence microscopy, digital imaging, and nucleic acid technology have prompted the div- sifcation of the original technique into an outstanding number of imaginative and useful applications, and thus have not only held back its outmoding but have also promoted its expansion into different areas of basic and applied research in the post-genomic era. The 34 chapters included in this book aim at portraying the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of va- ous applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology, and evolutionary st- ies. The book is divided in four parts: (I) Core Techniques, (II) Technical Advancements and Novel Adaptations, (III) Translational FISH: Applications for Human Genetics and Medicine, and (IV) Protocols for Model Organisms.
In Situ Hybridization
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Author : Julia M. Polak
language : en
Publisher: Oxford University Press, USA
Release Date : 1990
In Situ Hybridization written by Julia M. Polak and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990 with Literary Criticism categories.
Molecular biology and genetic engineering techniques have revolutionized our concepts of gene activity in cellular function. In situ hybridization has contributed to this revolution. It is the only technique which gives precise data on the content and distribution of DNA and mRNA in intactcells and tissues. It is now an invaluable test in understanding the pathophysiology of cellular functions. This book covers all aspects of in situ hybridization. It describes practical procedures and protocols, the scientific background, areas of application, and the limitations of the technology. The contributing authors are all experts. Each chapter contains up-to-date references, many colourillustrations, the latest advances, and prospects for the future. This practical guide will be invaluable to: molecular, cell, and developmental biologists; cyto- and molecular geneticists; clinicians, especially pathologists, biologists, and microbiologists; and scientists seriously interested inthe pathophysiology of cell function and its regulation.