Laser Capture In Microscopy And Microdissection
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Laser Capture In Microscopy And Microdissection
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Author :
language : en
Publisher: Elsevier
Release Date : 2002-11-01
Laser Capture In Microscopy And Microdissection written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-11-01 with Science categories.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. - Basic Principles, Specialized Uses, and Genetic Applications - LCM and its application in genomics and proteomics - Fluorescence in situ hybridization of LCM isolated nuclei from paraffin sections - Noncontact laser catapulting for the functional genomics and proteomics - Use of LCM for clonal analysis, in carcinoma analysis, to assess development, in complex tissue, in pathology, gene discovery, and more
Cell Imaging Techniques
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Author : Douglas J. Taatjes
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Cell Imaging Techniques written by Douglas J. Taatjes and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.
Morphology Methods
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Author : Ricardo V. Lloyd
language : en
Publisher: Springer Science & Business Media
Release Date : 2001-06-15
Morphology Methods written by Ricardo V. Lloyd and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-06-15 with Medical categories.
The past several decades have witnessed an impressive array of conceptual and techno logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure.
Pcr Applications
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Author : Michael A. Innis
language : en
Publisher: Academic Press
Release Date : 1999-05-11
Pcr Applications written by Michael A. Innis and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-05-11 with Science categories.
PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily.PCR Applications examines the latest developments in this field. It is the third book in the series, building on the previous publications PCR Protocols and PCR Strategies. The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom.Key Features* Focuses on gene discovery, genomics, and DNA array technology* Covers quantitative PCR techniques, including the use of standards and kinetic analysisincludes statistical refinement of primer design parameters* Ilustrates techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, and in situ PCREntries provide information on:* Nomenclature* Expression* Sequence analysis* Structure and function* Electrophysiology* Parmacology* Information retrieval
Neurohistology And Imaging Techniques
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Author : Radek Pelc
language : en
Publisher: Humana
Release Date : 2020-06-25
Neurohistology And Imaging Techniques written by Radek Pelc and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-06-25 with Medical categories.
This volume explores major light microscopic imaging modalities that can be used to view nervous tissue, and discusses the steps needed to use each of them, and ways to interpret the data. The chapters in this book cover topics such as atlasing of insect brain; neuroanatomical tracing through fluorochrome expression; fluorescent probes for amyloids; or optical clearing for ultramicroscopy of GFP-expressing tissues. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Neurohistology and Imaging Techniques is a valuable resource for both expert and novice users of major light microscopic imaging techniques, and those interested in exploring alternate imaging tools.
Protocols For Macroalgae Research
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Author : Bénédicte Charrier
language : en
Publisher: CRC Press
Release Date : 2018-04-17
Protocols For Macroalgae Research written by Bénédicte Charrier and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-04-17 with Science categories.
This book presents a wide range of tested and proven protocols relevant to a number of fields within biotechnology used in laboratory experiments in everyday phycological (seaweed) research. A major focus will be on bioenergy related aspects of this emerging technology. These protocols will be written in a clear and concise manner using simple language permitting even nonspecialist to adequately understand the significance of this research. It will also contain all necesssary notes and guidelines for successful execution of these experiments.
Lasers For Medical Applications
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Author : Helena Jelínková
language : en
Publisher: Woodhead Publishing
Release Date : 2013-09-30
Lasers For Medical Applications written by Helena Jelínková and has been published by Woodhead Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-09-30 with Technology & Engineering categories.
Lasers have a wide and growing range of applications in medicine. Lasers for Medical Applications summarises the wealth of recent research on the principles, technologies and application of lasers in diagnostics, therapy and surgery. Part one gives an overview of the use of lasers in medicine, key principles of lasers and radiation interactions with tissue. To understand the wide diversity and therefore the large possible choice of these devices for a specific diagnosis or treatment, the respective types of the laser (solid state, gas, dye, and semiconductor) are reviewed in part two. Part three describes diagnostic laser methods, for example optical coherence tomography, spectroscopy, optical biopsy, and time-resolved fluorescence polarization spectroscopy. Those methods help doctors to refine the scope of involvement of the particular body part or, for example, to specify the extent of a tumor. Part four concentrates on the therapeutic applications of laser radiation in particular branches of medicine, including ophthalmology, dermatology, cardiology, urology, gynecology, otorhinolaryngology (ORL), neurology, dentistry, orthopaedic surgery and cancer therapy, as well as laser coatings of implants. The final chapter includes the safety precautions with which the staff working with laser instruments must be familiar. With its distinguished editor and international team of contributors, this important book summarizes international achievements in the field of laser applications in medicine in the past 50 years. It provides a valuable contribution to laser medicine by outstanding experts in medicine and engineering.
Light And Video Microscopy
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Author : Randy O. Wayne
language : en
Publisher: Academic Press
Release Date : 2019-06-11
Light And Video Microscopy written by Randy O. Wayne and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-06-11 with Technology & Engineering categories.
Light and Video Microscopy, Third Edition provides a step-by-step journey through philosophy, psychology and the geometrical and physical optics involved in interpreting images formed by light microscopes. The book addresses the intricacies necessary to set up light microscopes that allow one to visualize transparent specimens and, in the process, quantitatively determine various physico-chemical properties of specimens. This updated edition includes the most recent developments in microscopy, ensuring that it continues to be the most comprehensive, easy-to-use, and informative guide on light microscopy. With its presentation of geometrical optics, it assists the reader in understanding image formation and light movement within the microscope. - Provides a fully-revised, updated resource on three-dimensional (3D) structures - Contains a new appendices on Diffraction Theory and Advanced Image Processing - Provides practical applications, lab exercises and case studies on the mathematics, physics and biology used in microscopy - Discusses bright field, dark field, phase-contrast, fluorescence, interference, differential interference and modulation contrast microscopes, oblique illumination and photomicrography
Theory And Practice Of Histological Techniques
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Author : John D. Bancroft
language : en
Publisher: Elsevier Health Sciences
Release Date : 2008-01-01
Theory And Practice Of Histological Techniques written by John D. Bancroft and has been published by Elsevier Health Sciences this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-01-01 with Medical categories.
This leading reference work on histological techniques is an essential and invaluable resource no matter what part you play in histological preparations and applications, whether you're a student or a highly experienced laboratory professional.
Molecular Biomethods Handbook
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Author : John M. Walker
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-11-04
Molecular Biomethods Handbook written by John M. Walker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-11-04 with Medical categories.
Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. Molecular Biomethods Handbook, 2nd Edition expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Part A of the book describes nucleic acid methods, such as gene expression profiling, microarray analysis and quantitative PCR. In Part B, protein and cell-based methods are outlined, in subjects ranging from protein engineering to high throughput screening. Written by a well-established panel of research scientists, Molecular Biomethods Handbook, 2nd Edition provides an up to date collection of methods used regularly in the authors’ own research programs. This book will prove to be an invaluable reference for those engaged in or entering the field of molecular biology, and will provide the necessary background for those interested in setting up and using the latest molecular techniques.