Pcr Applications
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Pcr Protocols
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Author : Michael A. Innis
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Pcr Protocols written by Michael A. Innis and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. Avoid contamination--with specific instructions on setting up your lab Avoid cumbersome molecular biological techniques Discover new applications
Pcr Protocols
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Author : Bruce A. White
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Pcr Protocols written by Bruce A. White and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR.
Clinical Applications Of Pcr
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Author : Rajyalakshmi Luthra
language : en
Publisher: Humana
Release Date : 2016-02-03
Clinical Applications Of Pcr written by Rajyalakshmi Luthra and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-02-03 with Science categories.
This updated volume explores a wide variety of clinical applications of PCR such as detecting DNA methylation, detection of viruses and protozoa in infectious diseases, estimation of gene copy number aberrations, primer extension coupled with mass spectroscopy, and high throughput NGS techniques. The application of PCR has shown incredible value in the study of genomics and transcriptomics, not only for discovery but also for routine clinical applications, and it forms the cornerstone of personalized medicine. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and authoritative, Clinical Applications of PCR, Third Edition serves as an ideal guide for researchers aiming to understand the principles behind each application and for their implementation in the laboratory.
Pcr Applications
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Author : Michael A. Innis
language : en
Publisher: Academic Press
Release Date : 1999-05-11
Pcr Applications written by Michael A. Innis and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-05-11 with Science categories.
PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily. PCR Applications examines the latest developments in this field. It is the third book in the series, building on the previous publications PCR Protocols and PCR Strategies. The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom. Key Features * Focuses on gene discovery, genomics, and DNA array technology * Covers quantitative PCR techniques, including the use of standards and kinetic analysis includes statistical refinement of primer design parameters * Ilustrates techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, and in situ PCR Entries provide information on: * Nomenclature * Expression * Sequence analysis * Structure and function * Electrophysiology * Parmacology * Information retrieval
Pcr Technology
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Author : Henry Erlich
language : en
Publisher: Springer
Release Date : 2015-12-31
Pcr Technology written by Henry Erlich and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-12-31 with Science categories.
This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.
Clinical Applications Of Pcr
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Author : Y. M. Dennis Lo
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-01
Clinical Applications Of Pcr written by Y. M. Dennis Lo and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-01 with Science categories.
The polymerase chain reaction (PCR) is one of the most important molecular biological methods ever devised, with numerous applications to cli- cal molecular medicine. Since its description in 1985, PCR has undergone tremendous improvements, and many variations on the basic PCR theme have been published. With such a large volume of PCR-related literature, a clinical scientist wishing to use the technique will have a difficult task loc- ing the relevant information to implement it effectively. There is thus clearly a need for an up-to-date volume with detailed protocols to facilitate the setting up of those techniques most relevant to clinical applications. Unlike some other books on this topic, Clinical Applications of PCR includes only methods that are of direct relevance in clinical settings. The book is organized in three parts: an introductory section, a section on general methodology, and a final section with specific clinical applications. The first section covers the basic principles of PCR and is most useful to those new to molecular diagnosis. The next chapter includes useful tips for setting up a PCR laboratory. Section 2 then outlines some of the most commonly used PCR-based techniques in molecular diagnosis. Section 3 includes carefully chosen examples that represent typical applications of PCR in diverse clinical fields, encompassing hematology, oncology, genetics, and microbiology.
Applications Of Pcr In Mycology
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Author : P. D. Bridge
language : en
Publisher: CABI
Release Date : 1998-01-01
Applications Of Pcr In Mycology written by P. D. Bridge and has been published by CABI this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-01-01 with Science categories.
Since the initial report of the amplification of specific DNA fragments using the polymerase chain reaction (PCR) in 1985, this technique has revolutionized molecular biology. It enables the production of large quantities of DNA from minute amounts of sample material, which can then be readily analyzed. This facility has had an enormous influence on the way both fundamental and diagnostic questions are approached and its use is now considered essential for molecular work in all branches of biology. The purpose of this book is to highlight the wide-ranging applications of PCR in pure and applied mycology and to increase understanding of its potential benefits. After a brief overview, a group of internationally-renowned mycologists give definitive descriptions of the use of PCR in their own specialized fields. These include fungal gene expression and cloning, taxonomy and speciation, fungal mycobionts, mycorrhizal fungi, entomopathogenic fungi, mycotoxin-producing fungi, diagnosis of fungal infections in animals, seed-borne diseases, fungal/plant interactions and applications with industrially-important fungi. Finally, potential future directions for PCR work in mycology are discussed.
Pcr Technology
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Author : Henry A. Erlich
language : en
Publisher: Oxford University Press, USA
Release Date : 1992
Pcr Technology written by Henry A. Erlich and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with DNA categories.
Polymerase chain reaction (PCR) technology is a revolutionary innovation which enables scientists to rapidly generate large amounts of genetic material from a slight trace which would otherwise be too small to analyze. With applications in both research and diagnostics, PCR is becoming a standard procedure in biotechnology and medical diagnostic laboratories. This book is an introduction and guide to the new technology, covering the basic methodologies and their applications in research and medicine, emphasizing practical aspects. Each chapter is written by pioneers in the field and most include detailed protocols and favorite PCR "recipes". Students and researchers in all areas of biotechnology and molecular biology will find this book the introduction to PCR they've been looking for.
Pcr
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Author : Mike McPherson
language : en
Publisher: Garland Science
Release Date : 2007-01-25
Pcr written by Mike McPherson and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-01-25 with Science categories.
A thoroughly updated version of the successful first edition with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections. Information on PCR applications in genomics and proteomics have been expanded and integrated throughout the text. There is also advice on available products and specific pointers to the most appropriate methods. As with the first edition, this will be an ideal practical introduction and invaluable guide to PCR and its applications.
Early Rapid And Sensitive Veterinary Molecular Diagnostics Real Time Pcr Applications
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Author : Erika Pestana
language : en
Publisher: Springer Science & Business Media
Release Date : 2010-01-18
Early Rapid And Sensitive Veterinary Molecular Diagnostics Real Time Pcr Applications written by Erika Pestana and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-01-18 with Science categories.
This book gives a comprehensive account of the practical aspects of Real time PCR and its application to veterinary diagnostic laboratories. The optimisation of assays to help diagnose livestock diseases is stressed and exemplified through assembling standard operating procedures from many laboratory sources. Theoretical aspects of PCR are dealt with as well as quality control features necessary to maintain an assured testing system. The book will be helpful to all scientists involved in diagnostic applications of molecular techniques, but is designed primarily to offer developing country scientists a collection of working methods in a single source. The book is an adjunct to the Molecular Diagnostic PCR Handbook published in 2005.