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Luminescence Spectroscopy And Imaging


Luminescence Spectroscopy And Imaging
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Luminescence Spectroscopy And Imaging


Luminescence Spectroscopy And Imaging
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Author : L. Nasdala
language : en
Publisher:
Release Date : 2013

Luminescence Spectroscopy And Imaging written by L. Nasdala and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with categories.




Fluorescence Lifetime Spectroscopy And Imaging


Fluorescence Lifetime Spectroscopy And Imaging
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Author : Laura Marcu
language : en
Publisher: CRC Press
Release Date : 2014-07-17

Fluorescence Lifetime Spectroscopy And Imaging written by Laura Marcu and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-07-17 with Technology & Engineering categories.


During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics explores the remarkable advances in time-resolved fluorescence techniques and their role in a wide range of biological and clinical applications. Broadly accessible, the book captures the state-of-the-art of fluorescence lifetime metrology and imaging and provides current perspectives on their applications to biomedical studies of intact tissues and medical diagnosis. The text introduces these techniques within the wider context of fluorescence spectroscopy and describes basic principles underlying current instrumentation for fluorescence lifetime imaging and metrology (FLIM). It also covers the wide range of methods, including single channel (point) spectroscopy, fluorescence lifetime imaging microscopy, and single- and multi-photon excitation. Edited by pioneers in this field, with contributions from leading experts, the book includes an overview of complementary techniques that help researchers beginning FLIM research. It offers a comprehensive treatment of fundamental principles, instrumentation, analytical methods, and applications. It also provides an overview of the label-free contrast available from lifetime measurements of tissue autofluorescence and the prospects for exploiting this for clinical applications and biomedical research including drug discovery.



Fluorescence Spectroscopy Imaging And Probes


Fluorescence Spectroscopy Imaging And Probes
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Author : Ruud Kraayenhof
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Fluorescence Spectroscopy Imaging And Probes written by Ruud Kraayenhof and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.


The increased use of fluorescence techniques is greatly enhanced by the improved instrumentation pioneered by inventive scientists and now made available commercially by several high-tech companies. Moreover, the design and development of many new molecular probes with higher selectivity for specific microenvironmental properties has stimulated many new researchers to employ fluorescence techniques for solving their problems. This topic book, the second in his series, reflects this exciting scientific progress and deals, among others, with new approaches and new probes in fluorescence spectroscopy, single molecule fluorescence, applications in biomembrane and enzyme studies and imaging of living cells.



Fluorescence Lifetime Spectroscopy And Imaging


Fluorescence Lifetime Spectroscopy And Imaging
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Author : Laura Marcu
language : en
Publisher:
Release Date : 2014-01-01

Fluorescence Lifetime Spectroscopy And Imaging written by Laura Marcu and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-01-01 with Diagnostic imaging categories.


During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics explores the remarkable advances in time-resolved fluorescence techniques and their role in a wide range of biological and clinical applications. Broadly accessible, the book captures the state-of-the-art of fluorescence lifetime metrology and imaging and provides current perspectives on their applications to biomedical studies of intact tissues and medical diagnosis. The text introduces these techniques within the wider context of fluorescence spectroscopy and describes basic principles underlying current instrumentation for fluorescence lifetime imaging and metrology (FLIM). It also covers the wide range of methods, including single channel (point) spectroscopy, fluorescence lifetime imaging microscopy, and single- and multi-photon excitation. Edited by pioneers in this field, with contributions from leading experts, the book includes an overview of complementary techniques that help researchers beginning FLIM research. It offers a comprehensive treatment of fundamental principles, instrumentation, analytical methods, and applications. It also provides an overview of the label-free contrast available from lifetime measurements of tissue autofluorescence and the prospects for exploiting this for clinical applications and biomedical research including drug discovery.



Topics In Fluorescence Spectroscopy


Topics In Fluorescence Spectroscopy
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Author : Joseph R. Lakowicz
language : en
Publisher: Springer Science & Business Media
Release Date : 1994-11-30

Topics In Fluorescence Spectroscopy written by Joseph R. Lakowicz and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994-11-30 with Science categories.


Time-resolved fluorescence spectroscopy is widely used as a research tool in bioch- istry and biophysics. These uses of fluorescence have resulted in extensive knowledge of the structure and dynamics of biological macromolecules. This information has been gained by studies of phenomena that affect the excited state, such as the local environment, quenching processes, and energy transfer. Topics in Fluorescence Spectroscopy, Volume 4: Probe Design and Chemical Sensing reflects a new trend, which is the use of time-resolved fluorescence in analytical and clinical chemistry. These emerging applications of time-resolved fluorescence are the result of continued advances in laser detector and computer technology. For instance, pho- multiplier tubes (PMT) were previously bulky devices. Miniature PMTs are now available, and the performance of simpler detectors is continually improving. There is also considerable effort to develop fluorophores that can be excited with the red/ne- infrared (NIR) output of laser diodes. Using such probes, one can readily imagine small time-resolved fluorometers, even hand-held devices, being used fordoctor’s office or home health care.



Fluorescence Methods And Applications


Fluorescence Methods And Applications
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Author : Otto S. Wolfbeis
language : en
Publisher: Wiley-Blackwell
Release Date : 2008-07

Fluorescence Methods And Applications written by Otto S. Wolfbeis and has been published by Wiley-Blackwell this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-07 with Science categories.


This volume features papers on new spectroscopic methods and techniques, the development and application of fluorescent probes, and new techniques and applications of fluorescence imaging. Specific areas include the following: fluorescence lifetime, fluorescence (in vivo) imaging, time-resolved fluorescence, luminescence anisotropy, fluorescent (NMIR) labels, luminescent lanthanides, fluorescent sensors and probes, fluorescence microscopy, FRET, fluorescent nanoparticles and dots, high-throughput screening, fluorescent bioassays, luminescence-based DNA technologies, FISH and immunohistochemistry, luminescence on metal surfaces, fluorescent proteins, upconversion, multiphoton fluorescence, confocal techniques, near-field and far-field techniques, single photon counting, fluorescence correlation spectroscopy (FCS), and flow cytometry. NOTE: Annals volumes are available for sale as individual books or as a journal. For information on institutional journal subscriptions, please visit www.blackwellpublishing.com/nyas. ACADEMY MEMBERS: Please contact the New York Academy of Sciences directly to place your order (www.nyas.org). Members of the New York Academy of Science receive full-text access to the Annals online and discounts on print volumes. Please visit www.nyas.org/membership/main.asp for more information about becoming a member.



Introduction To Fluorescence Sensing


Introduction To Fluorescence Sensing
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Author : Alexander P. Demchenko
language : en
Publisher: Springer Nature
Release Date : 2023-02-15

Introduction To Fluorescence Sensing written by Alexander P. Demchenko and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-02-15 with Medical categories.


Fluorescence is the most popular technique in chemical and biological sensing because of its ultimate sensitivity, high temporal and spatial resolution and versatility that enables imaging within the living cells. It develops rapidly in the directions of constructing new molecular recognition units, new fluorescence reporters and in improving sensitivity of response up to detection of single molecules. Its application areas range from control of industrial processes to environment monitoring and clinical diagnostics. This book provides systematic knowledge of basic principles in design of fluorescence sensing and imaging techniques together with critical analysis of recent developments. Being a guide for students and young researchers, it also addresses professionals involved in active basic and applied research. Making a strong link between education, research and product development, this book discusses prospects for future progress.



Proceedings Of Physiological Imaging Spectroscopy And Early Detection Diagnostic Methods


Proceedings Of Physiological Imaging Spectroscopy And Early Detection Diagnostic Methods
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Author : Randall L. Barbour
language : en
Publisher:
Release Date : 1993

Proceedings Of Physiological Imaging Spectroscopy And Early Detection Diagnostic Methods written by Randall L. Barbour and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with Medical categories.




Spectroscopy Of Luminescent Nanoparticles And Interactions With Organic Molecules For Imaging And Therapy


Spectroscopy Of Luminescent Nanoparticles And Interactions With Organic Molecules For Imaging And Therapy
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Author : Daniel Cooper
language : en
Publisher:
Release Date : 2014

Spectroscopy Of Luminescent Nanoparticles And Interactions With Organic Molecules For Imaging And Therapy written by Daniel Cooper and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with categories.


"The emergence of nanomaterials has had a profound effect on the scientific community, reflected in the ever-increasing number of dedicated 'nanojournals.' Biomedical applications of nanoparticles are numerous, and include imaging as luminescent probes or contrast agents, biosensing, drug and gene delivery and photodynamic therapy (PDT), among others. Luminescent semiconductor nanocrystals, known as quantum dots or QDs, were among the first varieties produced and remain the most popular choice for imaging due to their versatile optical properties. While it has long been recognized that QDs exhibit sensitivity to photoinduced processes involving interactions with organic molecules and oxygen species in aqueous environments, results in these complex systems are often contradictory. Two of the most popular QD compositions, CdSe/ZnS and CdTe, have redox potentials that permit interactions with relevant chemical species, frequently resulting in considerable fluctuations in their spectroscopic properties. By conjugating QDs to the small molecule electron donor dopamine (DA) and using time-resolved fluorescence spectroscopy, we have studied the dynamics governing photoenhancement of QD luminescence by biomolecule-mediated production of reactive oxygen species (ROS), implicating their involvement through oxygen deprivation and the action of antioxidants. This 'photosensitization' was further studied using electron paramagnetic resonance (EPR) and chemical assays to discern between types of ROS and the consistency of various probes. It has been proposed that dense luminescent nanoparticles could also be used in conjunction with radiation therapy to not only provide dose enhancement, but also as a means to improve delivery and indirect activation of PDT agents through scintillation energy transfer. While many scintillators have been studied in bulk for radiation detection and other purposes, the study of nanoscintillators is in its infancy. Current QD preparations, despite their notable photostability, have poor radiation hardness. Luminescent lanthanide-doped insulators are among the proposed alternatives, as they are relatively biocompatible and chemically stable. CexLa1-xF3 is a heavy, fast scintillator that shows promise for radiation-assisted PDT, but exhibits complex luminescence mechanisms that depend highly on the crystalline quality and Ce3+ dopant concentration. We report novel synthesis techniques and surface chemistry for Ce0.1La0.9F3/LaF3 and CeF3/LaF3 core/shell nanoparticles, and explore their mechanisms of photoluminescence as well as energy transfer to bound and unbound photosensitizer molecules in aqueous solutions. We found that Ce3+ excitation efficiently relays energy to photosensitizers through a multi-step cascade, results that have important implications for the design of nanoscintillator systems." --



Development Of A Multidimensional Luminescence Microscope And Its Application To Imaging And Spectroscopy Of Luminescent Defects In Diamond


Development Of A Multidimensional Luminescence Microscope And Its Application To Imaging And Spectroscopy Of Luminescent Defects In Diamond
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Author : Daniel Christopher Jones
language : en
Publisher:
Release Date : 2021

Development Of A Multidimensional Luminescence Microscope And Its Application To Imaging And Spectroscopy Of Luminescent Defects In Diamond written by Daniel Christopher Jones and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021 with categories.