Mammalian And Avian Transgenesis New Approaches
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Mammalian And Avian Transgenesis New Approaches
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Author : Shirley Pease
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-07-31
Mammalian And Avian Transgenesis New Approaches written by Shirley Pease and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-07-31 with Science categories.
Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds. The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.
Mammalian And Avian Transgenesis New Approaches
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Author : Carlos Lois
language : en
Publisher:
Release Date : 2006
Mammalian And Avian Transgenesis New Approaches written by Carlos Lois and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with Birds categories.
"Mammalian and Avian Transgenesis" presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds. The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.
Transgenesis And Genome Editing In Poultry
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Author : Young Sun Hwang
language : en
Publisher:
Release Date : 2018
Transgenesis And Genome Editing In Poultry written by Young Sun Hwang and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with Cooking categories.
The transgenic approach and precise editing of specific loci in the genome have diverse practical uses in animal biotechnology. Recent advances in genome-editing technology, including clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) have helped to generate highly valuable and quality-improved poultry. The production of transgenic and genome-edited birds mainly depends on primordial germ cells (PGCs), which are the progenitor cells of gametes, due to the unique system that is quite different from the mammalian system. This chapter introduces the basic physiology of avian PGCs and the latest PGC-mediated methodologies in transgenesis and genome editing of birds. Based on these techniques, future applications of precisely genome-modulated poultry are discussed to provide opportunities and benefits for humans.
Transgenesis
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Author : Thomas L. Saunders
language : en
Publisher: Springer Nature
Release Date : 2023-03-30
Transgenesis written by Thomas L. Saunders and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-03-30 with Science categories.
This detailed volume focuses on genotyping and validation in addition to information on how to produce gene edited cells and animals for research. Future advances in biomedical research will benefit greatly from the use of precise gene targeting of transgenes in the genome as CRISPR technology supersedes earlier methods that relied on random transgene integration, which this collection reflects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Transgenesis: Methods and Protocols serves as an ideal guide for researchers working with or working on genetically modified models.
Avian Immunology
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Author : Bernd Kaspers
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Avian Immunology written by Bernd Kaspers and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Medical categories.
The second edition of Avian Immunology provides an up-to-date overview of the current knowledge of avian immunology. From the ontogeny of the avian immune system to practical application in vaccinology, the book encompasses all aspects of innate and adaptive immunity in chickens. In addition, chapters are devoted to the immunology of other commercially important species such as turkeys and ducks, and to ecoimmunology summarizing the knowledge of immune responses in free-living birds often in relation to reproductive success. The book contains a detailed description of the avian innate immune system, encompassing the mucosal, enteric, respiratory and reproductive systems. The diseases and disorders it covers include immunodepressive diseases and immune evasion, autoimmune diseases, and tumors of the immune system. Practical aspects of vaccination are examined as well. Extensive appendices summarize resources for scientists including cell lines, inbred chicken lines, cytokines, chemokines, and monoclonal antibodies. The world-wide importance of poultry protein for the human diet, as well as the threat of avian influenza pandemics like H5N1 and heavy reliance on vaccination to protect commercial flocks makes this book a vital resource. This book provides crucial information not only for poultry health professionals and avian biologists, but also for comparative and veterinary immunologists, graduate students and veterinary students with an interest in avian immunology. With contributions from 33 of the foremost international experts in the field, this book provides the most up-to-date review of avian immunology so far Contains a detailed description of the avian innate immune system reviewing constitutive barriers, chemical and cellular responses; it includes a comprehensive review of avian Toll-like receptors Contains a wide-ranging review of the "ecoimmunology" of free-living avian species, as applied to studies of population dynamics, and reviews methods and resources available for carrying out such research
Manipulation Of The Avian Genome
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Author : Robert J. Etches
language : en
Publisher: Routledge
Release Date : 2019-03-14
Manipulation Of The Avian Genome written by Robert J. Etches and has been published by Routledge this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-03-14 with Medical categories.
Many genes have been cloned from chicken cells, and during the next decade numerous laboratories will be concentrating their resources in developing ways of using these tools. Manipulation of the Avian Genome contains the most recent information from leading research laboratories in the areas of developmental and molecular genetics of the chicken. This information was presented at the Keystone Symposium held at Lake Tahoe in March, 1991. The book discusses potential applications of emerging technology in basic science and poultry production. Various techniques for altering genomic DNA, such as microinjection, retroviral vectors, and lipofection are covered. Genome evaluation using DNA fingerprinting and conventional breeding techniques are presented.
Advanced Protocols For Animal Transgenesis
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Author : Shirley Pease
language : en
Publisher: Springer Nature
Release Date : 2011-08-30
Advanced Protocols For Animal Transgenesis written by Shirley Pease and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-08-30 with Science categories.
This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.
Histochemical And Cytochemical Methods Of Visualization
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Author : Jean-Marie Exbrayat
language : en
Publisher: CRC Press
Release Date : 2013-06-05
Histochemical And Cytochemical Methods Of Visualization written by Jean-Marie Exbrayat and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-05 with Science categories.
Histochemistry and cytochemistry are important fields for studying the inner workings of cells and tissues of the body. While visualization techniques have been in use for many years, new methods of detection developed recently help researchers and practitioners better understand cell activity. Histochemical and Cytochemical Methods of Visualization describes the essential techniques that can be used for histochemical investigations in both light and transmission electron microscopy. The book begins by discussing techniques in light microscopy. It reviews classical methods of visualization, histochemical and histoenzymatic methods, and methods used to visualize cell proliferation and apoptosis. Next, the book examines the cytochemical methods used in electron microscopy with traditional techniques, as well as more specialized methods. The final section provides an overview of image analysis and describes how image processing methods can be used to extract vital information. A 16-page insert supplies color illustrations to enhance the text. Techniques will continue to adapt to the latest technological innovations, allowing more and more precise quantification of images. These developments are essential to the biological as well as the medical sciences. This manual is a critical resource for novice and experienced researchers, technicians, and students who need to visualize what happens in the cell, the molecules expressed, the main enzymatic activities, and the repercussions of the molecular activities upon the structure of the cells in the body.
Fertility Preservation In Male Cancer Patients
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Author : John P. Mulhall
language : en
Publisher: Cambridge University Press
Release Date : 2013-02-21
Fertility Preservation In Male Cancer Patients written by John P. Mulhall and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-02-21 with Medical categories.
Men with cancer rendered infertile by surgery, chemotherapy, radiation and hormone therapy that are needed to control or cure their disease are increasingly being offered the chance to preserve their reproductive potential through artificial reproductive technologies. Cryopreservation of sperm and testicular tissue have increasingly helped boys and men preserve their fertility. There is a growing subspecialty within reproductive medicine aimed at fertility preservation in this population. Furthermore, strategies are being developed that may in the future revolutionize the approach to such patients. Written by international authorities in the field of fertility preservation, this comprehensive book is aimed at clinicians dealing with male cancer patients, in particular, urologists, andrologists, oncologists, pediatricians and nursing staff as well as clinicians in reproductive endocrinology. The text reviews the impact of cancers and their treatment on male fertility, the available fertility preservation strategies and post-treatment management.
The Cost Manual Of Laboratory Animal Care And Use
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Author : Bryan Howard
language : en
Publisher: CRC Press
Release Date : 2016-04-19
The Cost Manual Of Laboratory Animal Care And Use written by Bryan Howard and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-19 with Medical categories.
COST (European Cooperation in the field of Scientific and Technical Research) is an intergovernmental initiative in science and research intended to promote the coordination of nationally funded research in Europe. Four working groups discuss the housing of animals, their environmental needs, refinement of procedures, genetically modified animals, and cost-benefit analysis. Based on the activities of these working groups, this book provides the European best practices for individuals and institutions working with laboratory animals. The text also discusses the ethical evaluation of experiments and procedures involving animals.