Mass Spectrometry Of Glycoproteins
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Mass Spectrometry Of Glycoproteins
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Author : Arnaud Delobel
language : en
Publisher:
Release Date : 2021
Mass Spectrometry Of Glycoproteins written by Arnaud Delobel and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021 with Analytical chemistry categories.
This volume presents methods used for the analysis of glycoproteins at different levels--intact, subunit, glycopeptide, and monosaccharide--, and discusses and solves most analytical challenges that a scientist working on glycoproteins may come across. The chapters in this book cover topics such as the role of glycosylation on the properties of therapeutic glycoproteins; different analytical methods to characterize glycosylation, from the intact proteins to the glycan level, for both N-linked and O-linked glycoproteins; mass spectrometry imaging methodology for glycosylation analysis in tissues; approaches to characterizing glycosylation on cultured cells; and the use of cloud computing to deploy mass spectrometry data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Mass Spectrometry of Glycoproteins: Methods and Protocols is a valuable resource for scientists interested in learning more about this developing field.
Mass Spectrometry Based Glycoproteomics And Its Clinic Application
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Author : Haojie Lu
language : en
Publisher: CRC Press
Release Date : 2021-09-20
Mass Spectrometry Based Glycoproteomics And Its Clinic Application written by Haojie Lu and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-09-20 with Science categories.
As one of the most extensive and important protein post-translational modifications, glycosylation plays a vital role in regulating organisms and is associated with various physiological and pathological processes. Recently, researchers have focused on the need to characterize protein glycosylation sites, structures, and their degree of modification, to better understand their biological functions while also looking for potential biomarkers for diagnosis and treatment of disease. Mass spectrometry (MS) is one of the most powerful tools used to study biomolecules including glycoproteins and glycans. With the continuous development of glycoproteomics and glycomics based on MS analysis, more techniques have evolved and contribute to understanding the structure and function of glycoproteins and glycans. This book reviews advancements achieved in MS-based glycoproteomic analysis, including a wide range of analytical methodologies and strategies involved in selective enrichment; as well as qualitative, quantitative, and data analysis, together with their clinical applications. Significant examples are discussed to illustrate the principles, laboratory protocols, and advice for key implementation to ensure successful results. Mass Spectrometry–Based Glycoproteomics and Its Clinic Application will serve as a valuable resource to elucidate new techniques and their applications for students, postdocs, and researchers working in proteomics, glycoscience, analytical chemistry, biochemistry, and clinical medicine. Editor: Haojie Lu is a professor at Fudan University, specializing in proteomics based on mass spectrometry with particular emphasis on novel technologies for separation and identification of low-abundant proteins and post-translationally modified proteins (including glycosylation), as well as relative and absolute quantification methods for proteomics.
Protein Glycosylation Advances In Identification Characterization And Biological Function Elucidation Using Mass Spectrometry
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Author : Ganglong Yang
language : en
Publisher: Frontiers Media SA
Release Date : 2022-03-14
Protein Glycosylation Advances In Identification Characterization And Biological Function Elucidation Using Mass Spectrometry written by Ganglong Yang and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-03-14 with Science categories.
Mass Spectrometry Of Glycoproteins
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Author : Arnaud Delobel
language : en
Publisher: Humana
Release Date : 2021-04-28
Mass Spectrometry Of Glycoproteins written by Arnaud Delobel and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-04-28 with Science categories.
This volume presents methods used for the analysis of glycoproteins at different levels—intact, subunit, glycopeptide, and monosaccharide--, and discusses and solves most analytical challenges that a scientist working on glycoproteins may come across. The chapters in this book cover topics such as the role of glycosylation on the properties of therapeutic glycoproteins; different analytical methods to characterize glycosylation, from the intact proteins to the glycan level, for both N-linked and O-linked glycoproteins; mass spectrometry imaging methodology for glycosylation analysis in tissues; approaches to characterizing glycosylation on cultured cells; and the use of cloud computing to deploy mass spectrometry data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Mass Spectrometry of Glycoproteins: Methods and Protocols is a valuable resource for scientists interested in learning more about this developing field.
Mass Spectrometry
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Author : Michael A. Madson
language : en
Publisher: Elsevier
Release Date : 2016-05-06
Mass Spectrometry written by Michael A. Madson and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-05-06 with Science categories.
Mass Spectrometry: Techniques for the Structural Characterization of Glycans presents new methods for conducting detailed carbohydrate qualitative analysis—arming analytical chemists, pharmaceutical scientists, and food scientists with a quick reference that will allow them to determine the structures of carbohydrates molecules. As there is a need in the scientific community for content specific to structural determination and analysis of new glycoprotein drug, and because structure-activity analysis requires a structural determination of the N- and O-linked oligosaccharides linked to glycol-proteins, this book provides the relevant research that are necessary for advances and new outcomes in this area of study. Authored by an analytical chemist with more than 30 years of experience in research and industry Serves as a quick reference in mass spectral analysis and carbohydrates Includes more than 60 figures to aid in the retention of key concepts
Mass Spectrometry Of Glycoproteins
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Author : Jennifer J. Kohler
language : en
Publisher: Humana Press
Release Date : 2016-05-01
Mass Spectrometry Of Glycoproteins written by Jennifer J. Kohler and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-05-01 with Science categories.
This comprehensive new resource in Springer s Methods in Molecular Biology series features contributions from leading researchers who provide expert advice and reproducible, cutting-edge protocols for examining glycoproteins through mass spectrometry."
Recombinant Glycoproteins
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Author : Steven B. Bradfute
language : en
Publisher: Springer Nature
Release Date :
Recombinant Glycoproteins written by Steven B. Bradfute and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.
Functional And Structural Proteomics Of Glycoproteins
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Author : Raymond J. Owens
language : en
Publisher: Springer Science & Business Media
Release Date : 2010-12-02
Functional And Structural Proteomics Of Glycoproteins written by Raymond J. Owens and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-12-02 with Science categories.
It has been predicted that nearly half of all human proteins are glycosylated indicating the significance of glycoproteins in human health and disease. For example, the glycans attached to proteins have emerged as important biomarkers in the diagnosis of diseases such as cancers and play a significant role in how pathogenic viruses gain entry into human cells. The study of glycoproteins has now become a truly proteomic science. In the last few years, technology developments including in silico methods, high throughput separation and detection techniques have accelerated the characterization of glycoproteins in cells and tissues. Glyco-engineering coupled to rapid recombinant protein production has facilitated the determination of glycoprotein structures key to exploring and exploiting their functional roles. Each chapter in this volume is written by experts in the field and together provide a review of the state of the art in the emerging field of glycoproteomics.
Analysis Of Protein Post Translational Modifications By Mass Spectrometry
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Author : John R. Griffiths
language : en
Publisher: John Wiley & Sons
Release Date : 2016-10-12
Analysis Of Protein Post Translational Modifications By Mass Spectrometry written by John R. Griffiths and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-10-12 with Science categories.
Covers all major modifications, including phosphorylation, glycosylation, acetylation, ubiquitination, sulfonation and and glycation Discussion of the chemistry behind each modification, along with key methods and references Contributions from some of the leading researchers in the field A valuable reference source for all laboratories undertaking proteomics, mass spectrometry and post-translational modification research
Handbook Of Glycomics
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Author : Richard D. Cummings
language : en
Publisher: Academic Press
Release Date : 2009-10-19
Handbook Of Glycomics written by Richard D. Cummings and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-10-19 with Science categories.
The Handbook of Glycomics provides the first comprehensive overview of the emerging field of glycomics, defined as the study of all complex carbohydrates in an organism or cell ("the glycome"). Beginning with analytic approaches and bioinformatics, this work provides a detailed discussion of relevant databases, data integration, and analysis. It then moves on to a discussion of specific model organism and pathogen glycomes followed by therapeutic approaches to human disorders of glycosylization. Structure and function of glycomes are included along with state-of-the-art technologies and systems approaches to the analysis of glycans. Synthesizes contributions from experts in biology, chemistry, bioinformatics, biotechnology, and medicine Highlights chapters devoted to chemical synthesis, cancer glycomics and immune cell glycomics Includes discussions of proteomics, mass spectrometry, NMR, array technology, and transcriptomics analytic approaches