Methods In Molecular Biology New Nucleic Acid Techniques
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Methods In Molecular Biology New Nucleic Acid Techniques
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Author : John M. Walker
language : en
Publisher:
Release Date : 1984
Methods In Molecular Biology New Nucleic Acid Techniques written by John M. Walker and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Molecular biology categories.
The Nucleic Acid Protocols Handbook
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Author : Ralph Rapley
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-06-29
The Nucleic Acid Protocols Handbook written by Ralph Rapley and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-06-29 with Science categories.
A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field."
Nucleic Acid Detection
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Author : Dmitry M. Kolpashchikov
language : en
Publisher: Humana
Release Date : 2016-08-23
Nucleic Acid Detection written by Dmitry M. Kolpashchikov and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Medical categories.
In Nucleic Acid Chemistry: Methods and Protocols, expert researches in the field detail techniques and approaches for the detection of DNA and RNA. These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of isothermal amplification techniques, assays with visual or electric signals for point-of-care diagnostics, improvement of fluorescent in situ hybridization, and new signal amplification techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Nucleic Acid Chemistry: Methods and Protocols seeks to aid scientists in the further study of detection for DNA and RNA.
Techniques In Molecular Biology
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Author : J.M. Walker
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Techniques In Molecular Biology written by J.M. Walker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.
Protocols For Nucleic Acid Analysis By Nonradioactive Probes
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Author : Elena Hilario
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Protocols For Nucleic Acid Analysis By Nonradioactive Probes written by Elena Hilario and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Molecular Biology
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Author : Susan J. Karcher
language : en
Publisher: Academic Press
Release Date : 1995-11-28
Molecular Biology written by Susan J. Karcher and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-11-28 with Science categories.
This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning. Includes a glossary of over 200 terms important for understanding molecular biology Uses an inexpensive source of eukaryotic cells - great for schools on a budget Includes Methods Locator that provides instant access to the latest methods Contain clearly written, easy-to-follow, student-tested instructions: Sterile techniques Phage titration Gel electrophoresis of DNA Restriction enzyme digestion Plasmid isolation Transformation of E. Coli Recombinant DNA cloning Nick translation labeling Nonradioactive primer labelling Nonradioactive DNA detection Southern blotting Colony hybridization Purification of plant DNA RNA purification Northern blotting Purification of poly A+ RNA Polymerase chain reaction (PCR)
Basic Methods In Molecular Biology
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Author : Leonard Davis
language : en
Publisher: Elsevier
Release Date : 2012-12-02
Basic Methods In Molecular Biology written by Leonard Davis and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Basic Methods in Molecular Biology discusses the heart of the most recent revolution in biology—the development of the technology of genetics. The achievements in this field have simply changed what biologists do and, perhaps even more important, the way they think. Moreover, never before have scientists from such a broad range of disciplines rushed into such a small and slightly arcane field to learn and carry off a bit of the technology. This book comprises 21 chapters, opening with three introductory ones that discuss the basics of molecular biology; the tools of the molecular biologist; and general preparations, procedures, and considerations for use of the book. The following chapters then discuss cloning vectors and bacterial cells; preparation of DNA from eukaryotic cells; probing nucleic acids; plasmid DNA preparation; DNA restriction fragment preparation; purification of DNA; and preparation and analysis of RNA from eukaryotic cells. Other chapters cover preparation of DNA from bacteriophage clones; cloning DNA from the eukaryotic genome; subcloning into plasmids; M13 cloning and sequencing; further characterization of cloned DNA; transfection of mammalian cells in culture; protein methods; general methods; and specialized methods. This book will be of interest to practitioners in the fields of biology and molecular genetics.
Techniques In Molecular Biology
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Author : John M. Walker
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Techniques In Molecular Biology written by John M. Walker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly intro duced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have con tributed a wide range of new techniques. In Volume 1, published in 1983, we introduced the reader to a selection of the more advanced analytical and preparative techniques which we considered to be frequently used by research workers in the field of molecular biology. In choosing techniques for Volume 1 we obviously had to be selective and were unable to cover as broad a spectrum of techniques as we would have liked. However, the pro duction of Volume 2 has allowed us to develop the theme initiated in Volume 1 and also expand to include a wider range of subject areas. As with Volume 1, the majority of chapters relate to nucleic acid method ology, but we have also covered immunological methodology and protein 1. Obviously, we purification techniques that were not included in Volume see Volume 2 as simply a continuation of Volume 1. As with Volume 1, a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new termin ology, a glossary of terms has been included.
Oligonucleotide Synthesis
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Author : Piet Herdewijn
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Oligonucleotide Synthesis written by Piet Herdewijn and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Medical categories.
A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.
Methods In Dna Amplification
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Author : Ulrich Finckh
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Methods In Dna Amplification written by Ulrich Finckh and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
The polymerase chain reaction (PCR) - an in Vitro techniques for producing large amounts of a specific DNA fragment - has rapidly become established as one of the most important, impressive and fascinating methods of molecular biology as well as clinical diagnostics. In the seven years since'the technique was published, it has had a major impact on medical research. However, as there are still problems in instruments, standardized protocols for diagnostic applications and unsolved difficulties to avoid cross-contaminations on the one hand and on the other hand the even present question of how to interpret the biological value of a PCR result, most clinicians prefer to further wait until these topics are clarified. It is the aim of this book to give the reader lab-proven protocols from experienced scientists as well as a general introduction to alternative DNA-amplification procedures and their possible usage such as the NASBA or LCR. This book is divided into four major parts to provide a theoretical (first and second section) and a practical framework for a better understanding of the new technology. In the first part we provide an up-to-date summary of basic problems in this rapidly evolving field. We demonstrate, for example how to use fixed tissue materials and how to quantify PCR products as well as how to prepare nucleic acids in a safe, convenient and proper way, or even how to sequence directly PCR products for the analysis of the DNA structure.
