New And Emerging Proteomic Techniques

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New And Emerging Proteomic Techniques

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Author : Dobrin Nedelkov
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04

New And Emerging Proteomic Techniques written by Dobrin Nedelkov and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.

Leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents. The methods span the entire spectrum of top-down and bottom-up approaches, including microarrays, gels, chromatography, and affinity separations, and address every aspect of the human proteome, both quantitatively and qualitatively. The techniques of protein detection utilized are diverse and range from fluorescence and resonance light scattering to surface plasmon resonance and mass spectrometry. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Principles Of Genomics And Proteomics

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Author : Rakeeb Ahmad Mir
language : en
Publisher: Elsevier
Release Date : 2023-01-25

Principles Of Genomics And Proteomics written by Rakeeb Ahmad Mir and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-01-25 with Science categories.

Principles of Genomics and Proteomics is the perfect reference for graduate students and researchers in these areas to understand its principles and execute precise and reproducible experiments. Following an introductory chapter, the book dives into proper research, including genome mapping. Experiments covered in the book span from Sangers Sequencing, Shotgun sequencing, SAGE analysis, DNA footprinting, Gel retardation, ChIP, and protein resolution methods, including PAGE, 2D gel electrophoresis and isoelectric focusing. Biophysical techniques are also described in detail, including ultraviolet and visible light spectroscopy, fluorescence spectroscopy, NMR and X-ray diffraction. A final proteome analysis is dedicated to functional analysis. Other chapters cover applications of omics technologies broadly. This book is the perfect reference for genetics labs around the world. Graduate students will benefit from the structured and detailed coverage of methods and established researchers will benefit from the book for staff training in research and may find it particularly helpful in enhancing reproducibility of experiments. Provides a comprehensive accounting of the technical aspects of genomic and proteomic analysis and applications in living organisms Includes detailed and standardized coverage of basic research methods in areas covered Presents advanced applications of genomics and proteomics that are highlighted with major significance in medical and agricultural fields

Proteomics

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Author : Shafat Ali
language : en
Publisher: Elsevier
Release Date : 2023-02-14

Proteomics written by Shafat Ali and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-02-14 with Science categories.

Proteomics: A Promising Approach for Cancer Research provides an updated overview of scientific knowledge, achievements and findings in the field of cancer proteomics. The book discusses topics such as the use of proteomics in cancer biology and drug discovery, its role in surgical oncology, applications of mass spectrometry, target proteomics, single-cell proteomics, and next-generation proteomics. In addition, it discusses proteomics and phosphor-proteomics in cancer precision medicine; translation of proteomics research into clinical application; and challenges and future developments of the field. This will be a valuable resource for cancer researchers, oncologists, graduate students, and members of biomedical field who are interested in the potential of proteomics in cancer research and treatment. The field of cancer proteomics is very dynamic, with emerging trends related to clinical solutions developed in recent years, therefore this book's content helps readers get up-to-speed on the topic to easily apply learnings into their research or clinical practice. - Provides up-to-date information on current cancer proteomics research developed globally - Presents basic research aspects to clinical implications of proteomics on cancer diagnosis and potential treatments - Discusses challenges and future developments of the field to leverage further research and applicability in clinical setting

Yac Protocols

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Author : Alasdair MacKenzie
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-10-26

Yac Protocols written by Alasdair MacKenzie and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-26 with Science categories.

It is now 10 years since the first edition of YAC Protocols was published in 1996. YAC Protocols was first produced to address the huge demand within the research community for a lab-based text that described in detail the wide range of uses for large insert yeast artificial chromosome (YAC) DNA clones. In doing this, the original editor, David Markie, and the many different contri- tors who provided descriptions of the protocols they used and developed, did a magnificent job. Indeed many of the techniques described within the first e- tion require little change and have stood up admirably to the test of time. Since the first edition, the use of YACs has proved invaluable for addressing a wide range of new biological problems ranging from those of basic biochemistry to assisting in the mapping and sequencing of the human genome. The requirement for a second edition of YAC Protocols was prompted by a number of major advances in biology since the publication of the first edition. These advances have included the sequencing of the human genome, and the genomes of a wide variety of other organisms, and the increased use of transgenic animals for understanding the molecular basis of human and animal disease.

Cytochrome P450 Protocols

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Author : Ian R. Phillips
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04

Cytochrome P450 Protocols written by Ian R. Phillips and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.

For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source.

Embryonic Stem Cell Protocols

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Author : Kursad Turksen
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04

Embryonic Stem Cell Protocols written by Kursad Turksen and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.

Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume one, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. The second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.

Advances In Chromatography Volume 49

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Author : Eli Grushka
language : en
Publisher: CRC Press
Release Date : 2016-04-19

Advances In Chromatography Volume 49 written by Eli Grushka and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-19 with Medical categories.

Advances in Chromatography is a venerable series that has reported on the latest state-of-the-art developments in the field for the past four decades. The newest installment, Volume 49, continues the tradition of compiling the work of expert contributors who present timely and cutting edge reviews of current and emerging methods and applications in

New Aspects Of Meat Quality

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Author : Peter P. Purslow
language : en
Publisher: Woodhead Publishing
Release Date : 2022-08-23

New Aspects Of Meat Quality written by Peter P. Purslow and has been published by Woodhead Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-08-23 with Technology & Engineering categories.

New Aspects of Meat Quality, Second Edition continues to be the leading source of scientific information for what constitutes meat quality for consumers, marketers and producers in the 21st century. The book includes traditional measures of meat quality such as texture, water holding, color, flavor/aroma, safety/microbiology and processing characteristics as well as quality assurance schemes, organic/free range, ethical meat production, and the desirability of genetically modified organisms, amongst others. Users will find comprehensive coverage on developments in our understanding of how muscle structure affects the eating qualities of cooked meat, along with techniques for measuring, predicting and producing meat quality. In addition, the book covers how these new techniques help us minimize variability in eating quality and/or maximize value. The book's final section identifies the current qualities of consumer and public perceptions and what is sustainable, ethical, desirable and healthy in meat production and consumption. - Provides the latest research techniques and developments presented by top researchers in the field - Covers new aspects of meat quality with the same scientific authority as texts on traditional meat quality value - Includes five new chapters that cover the role of proteolysis, meat flavor, meat bi-products, and meat and public health

Nuclear Reprogramming

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Author : Steve Pells
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04

Nuclear Reprogramming written by Steve Pells and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.

A wide-ranging collection of readily reproducible methods for performing nuclear reprogramming by nuclear transfer in several different species, by fusion through both chemical treatment and electrically shocking cells, and by in vivo treatment of cells with cell extracts. Several methods of monitoring nuclear reprogramming are also presented, including the use of transgenic markers, activation of telomerase as an ES-specific marker, light and electron microscopic observation of structural changes in the nucleus, and verification of surface marker expression and the differentiation potential of stem cells. Biochemical methods are provided for the examination of chromatin protein modifications, nucleosomal footprinting, transcription factor binding, and the study of DNA methylation changes both at the specific locus level and at the level of the whole nucleus.

In Situ Hybridization Protocols

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Author : Ian A. Darby
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04

In Situ Hybridization Protocols written by Ian A. Darby and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.

The technique of in situ hybridization, in its various forms, has been used routinely in many laboratories for a number of years. In the post-genome era, gene arrays and proteomics have allowed us to identify hitherto unknown unrecognized pathways and mechanisms. However, rather than diminish the importance of in situ hybridization, the now widespread use of screening te- nologies has increased the need to temporally and spatially localize the dist- bution of mRNA expression. Our intention, in In Situ Hybridization Protocols is to provide ample inf- mation for novices planning to set up the in situ hybridization technique and use it in their laboratory for the first time, as well as giving updates of recent developments for those laboratories where in situ hybridization techniques are already in use. Despite its widespread significance, in situ hybridization has retained a re- tation as one of the more difficult and capricious molecular biological te- niques. This may in part be because of the hybrid nature of the technique, which often requires a mixture of molecular biological and histological skills. The two techniques are usually taught and acquired in different streams of biolo- cal science. The step-by-step and detailed protocols provided in In Situ Hybridization Protocols by researchers active in the field should make it p- sible for both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use the technique s- cessfully in their laboratories.