Pcr 2

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Pcr 2

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Author : M. J. McPherson
language : en
Publisher:
Release Date : 1995

Pcr 2 written by M. J. McPherson and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with DNA replication categories.

PCR (polymerase chain reaction) is now one of the most widely used of basic molecular biology techniques and is an indispensable research tool for the molecular biologist. The basic PCR technique provides the cornerstone for in vitro DNA amplification - allowing the researcher to producelarge quantities of DNA from minute amounts of starting material.PCR: A Practical Approach Volume 2 is not a revised version of PCR: A Pracical Approach, but sets out to address some of the exciting new applications fo PCR including cDNA cloning, mRNA quantitation and expression of proteins fromPCR products, genomic DNA mapping and fingerprinting, and mutational analysis.

Pcr Methods In Foods

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Author : John Maurer
language : en
Publisher: Springer Science & Business Media
Release Date : 2006-11-22

Pcr Methods In Foods written by John Maurer and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-11-22 with Technology & Engineering categories.

This book will introduce non-molecular biologists to diagnostic PCR-based te- nologies for the detection of pathogens in foods. By the conclusion of this book, the reader should be able to: 1) understand the principles behind PCR including real-time; 2) know the basics involved in the design, optimization, and imp- mentation of PCR in food microbiology lab setting; 3) interpret results; 4) know limitations and strengths of PCR; and 5) understand the basic principles behind a new fledgling technology, microarrays and its potential applications in food microbiology. This book will provide readers with the latest information on PCR and microarray based tests and their application towards the detection of bacterial, protozoal and viral pathogens in foods. Figures, charts, and tables will be used, where appropriate, to help illustrate concepts or provide the reader with useful information or resources as an important starting point in bringing molecular diagnostics into the food microbiology lab. This book is not designed to be a “cookbook”PCR manual with recipes and step-by-step instructions but rather serve as a primer or resource book for students, faculty, and other professionals interested in molecular biology and its integration into food safety. v Table of Contents Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Chapter 1. PCR Basics Amanda Fairchild, M. S. , Margie D. Lee DVM, Ph. D. , and John J. Maurer, Ph. D. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Chapter 2. The Mythology of PCR: A Warning to the Wise John J. Maurer, Ph. D. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 Chapter 3.

Clinical Applications Of Pcr

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Author : Y. M. Dennis Lo
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-01

Clinical Applications Of Pcr written by Y. M. Dennis Lo and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-01 with Science categories.

The polymerase chain reaction (PCR) is one of the most important molecular biological methods ever devised, with numerous applications to cli- cal molecular medicine. Since its description in 1985, PCR has undergone tremendous improvements, and many variations on the basic PCR theme have been published. With such a large volume of PCR-related literature, a clinical scientist wishing to use the technique will have a difficult task loc- ing the relevant information to implement it effectively. There is thus clearly a need for an up-to-date volume with detailed protocols to facilitate the setting up of those techniques most relevant to clinical applications. Unlike some other books on this topic, Clinical Applications of PCR includes only methods that are of direct relevance in clinical settings. The book is organized in three parts: an introductory section, a section on general methodology, and a final section with specific clinical applications. The first section covers the basic principles of PCR and is most useful to those new to molecular diagnosis. The next chapter includes useful tips for setting up a PCR laboratory. Section 2 then outlines some of the most commonly used PCR-based techniques in molecular diagnosis. Section 3 includes carefully chosen examples that represent typical applications of PCR in diverse clinical fields, encompassing hematology, oncology, genetics, and microbiology.

The Polymerase Chain Reaction

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Author : Kary B. Mullis
language : en
Publisher: Springer Science & Business Media
Release Date : 1994

The Polymerase Chain Reaction written by Kary B. Mullis and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Medical categories.

One: Methodology.- I. Basic Methodology.- 1. Manipulation of DNA by PCR.- 2. Cloning PCR Products.- 3. Optimization of Multiplex PCRs.- 4. Preparation of Nucleic Acids for Archival Material.- 5. PCR Amplification of Viral DNA and Viral Host Cell mRNAs in Situ.- II. Quantitation.- 6. Quantitative PCR: An Overview.- 7. Quantification of DNAs by the Polymerase Chain Reaction Using an Internal Control.- 8. RT-PCR and mRNA Quantitation.- 9. Analysis of Human T-Cell Repertoires by PCR.- III. Nonisotopic Detection.- 10. Ultrasensitive Nonradioactive Detection of PCR Reactions: An Overview.- 11. Fluorescent Detection Methods for PCR Analysis.- 12. Enzyme-Labeled Oligonucleotides.- 13. Application of the Hybridization Protection Assay (HPA) to PCR.- IV. Instrumentation.- 14. PCR Instrumentation: Where Do We Stand?.- 15. Rapid Cycle DNA Amplification.- 16. Automating the PCR Process.- V. Sequencing.- 17. PCR and DNA Sequencing.- 18. Phage Promoter-Based Methods for Sequencing and Screening for Mutations.- 19. Capture PCR: An Efficient Method for Walking Along Chromosomal DNA and cDNA.- Two: Applications.- I. General Applications.- 20. In Vitro Evolution of Functional Nucleic Acids: High-Affinity RNA Ligands of the HIV-1 rev Protein.- 21. The Application of PCR to Forensic Science.- 22. Recreating the Past by PCR.- 23. Nonbiological Applications.- II. Genetic Analysis.- 24. RT-PCR and Gene Expression.- 25. Fingerprinting Using Arbitrarily Primed PCR: Application to Genetic Mapping, Population Biology, Epidemiology, and Detection of Differentially Expressed RNAs.- 26. Genetics, Plants, and the Polymerase Chain Reaction.- III. Assessment of Therapy Effectiveness.- 27. PCR Assessment of the Efficacy of Therapy in Philadelphia Chromosome-Positive Leukemias.- 28. The Detection of Minimal Residual Disease (MRD) in Acute Lymphoblastic Leukemia Using Clone-Specific Probes Directed against V(D)J Junctional Sequences.- 29. Assessment of Therapy Effectiveness: Infectious Disease.- 30. Gene Therapy.- IV. Diagnostics.- 31. PCR and Cancer Diagnostics: Detection and Characterization of Single Point Mutations in Oncogenes and Antioncogenes.- 32. Clinical Applications of the Polymerase Chain Reaction.- 33. Infectious Diseases.- Three: PCR and the World of Business.- 34. PCR in the Marketplace.- 35. PCR and Scientific Invention: The Trial of DuPont vs. Cetus.

The Pcr Technique

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Author : Ulf Gyllensten
language : en
Publisher: Eaton Publishing Company/Biotechniques Books
Release Date : 1997

The Pcr Technique written by Ulf Gyllensten and has been published by Eaton Publishing Company/Biotechniques Books this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Medical categories.

Pcr Detection Of Microbial Pathogens

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Author : Mark Wilks
language : en
Publisher: Humana Press
Release Date : 2012-06-30

Pcr Detection Of Microbial Pathogens written by Mark Wilks and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-06-30 with Science categories.

The Polymerase Chain Reaction Pcr For Human Viral Diagnosis

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Author : Jonathan P. Clewley
language : en
Publisher: CRC Press
Release Date : 1994-10-27

The Polymerase Chain Reaction Pcr For Human Viral Diagnosis written by Jonathan P. Clewley and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994-10-27 with Science categories.

The polymerase chain reaction (PCR) is the most sensitive method for revealing the presence of otherwise undetectable quantities of the genome of RNA or DNA of human viruses. The Polymerase Chain Reaction (PCR) for Human Viral Diagnosis addresses the urgent need to use this revolutionary technology in reference and routine diagnostic laboratories. It informs the molecular biologist of the most appropriate clinical uses for PCR and educates the clinician and medical virologist about the subtleties and benefits of gene amplification. The reader is given an understanding and appreciation of the principles of PCR and how, why, and where it should be applied. The book explains the principles behind PCR and its role in the diagnostic and public health laboratory. The application of PCR to the detection and investigation of viral latency and persistence is presented by the originators of in situ amplification. There are individual contributions from experts in their respective fields on the detection, characterization, and analysis by PCR of gastroenteritis viruses, hepatitis viruses, herpesviruses, rhinoviruses, enteroviruses, flaviviruses, polyomaviruses, human immunodeficiency virus (HIV), human T-lymphotropic virus types I and II (HTLV-I and II); and measles, mumps, rubella, influenza, rabies, and B19 viruses.

Development Of Rt Pcr Based Diagnosis Of Sars Cov 2

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Author : Vasudeo Zambare
language : en
Publisher:
Release Date : 2022

Development Of Rt Pcr Based Diagnosis Of Sars Cov 2 written by Vasudeo Zambare and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022 with Electronic books categories.

In the 2020, COVID-19 pandemic disease created an havoc situation world widely and mainly caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). It has been challenging task for researchers, scientists and medico-pharmaceutical organisations to find out rapid and reliable diagnosis methods. Among the all testing services, a Reverse Transcription Polymerase Chain Reaction (RT-PCR) is the more accurate, rapid and authenticated molecular technique used for most of the diagnosis of major diseases. It has been a global priority to fix the rapid diagnosis method to combat against the pandemic COVID-19. Thus, the present chapter mainly focussing on the progress of RT-PCR method development though various processes of data collection on isolation of whole genome sequence, its primer and method designing. In this scenario, India suddenly become the global leader for vaccine development and hence the challenges and RT-PCR kit development in India and rest of the world has been be discussed. World wide many Government and private agencies and industries have taken an initiative for diagnosis of SARS-CoV-2 hence this chapter also summarised the scope of RT-PCR to combat pandemic situation in future.

Pcr Based Diagnostics In Infectious Disease

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Author : Garth D. Ehrlich
language : en
Publisher: Wiley-Blackwell
Release Date : 1994

Pcr Based Diagnostics In Infectious Disease written by Garth D. Ehrlich and has been published by Wiley-Blackwell this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Medical categories.

Designed as both a comprehensive reference and a practical laboratory guide, the book is divided into three major interrelated sections: Section 1, the Clinical PCR Laboratory, is an introduction to PCR technology and the establishment of an effective lab; Section 2, PCR in Clinical Diagnostics, describes the role of PCR in diagnosing specific diseases, from the Epstein-Barr virus through sexually transmitted diseases; and Section 3, PCR-Based Protocols, explains the use of PCR technology in detecting specific organisms.

Rapid Cycle Real Time Pcr

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Author : Carl Wittwer
language : en
Publisher: Springer Science & Business Media
Release Date : 2004

Rapid Cycle Real Time Pcr written by Carl Wittwer and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Medical categories.

Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I allow product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms. The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and analysis in the fields of Genetics and Oncology. Molecular analysis has never been easier! TOC: Methods 1 Onno Bakker, Academic Medical Centre Amsterdam, NL Housekeeping Genes: A Gold Standard? 2 Weisser/ Schnittger, Klinikum Grosshadern München, Germany The choice of house keeping genes in MRD-quantification of t(8;21) positive AML 3 Ronald H. Lekanne-Deprez, Dep of Anatomie & Embryologie, Amsterdam, The Netherlands Quantification of mRNA Using Linear Regression of Log- Linear PCR Data-Points as an Alternative for the Standard Curve Approach 4 Jochen Wilhelm, University Giessen Estimation of Genome Sizes by Quantitative Real-Time PCR Applications Regulation and Development 5 N. Neubauer, University of Copenhagen, Biokemisk Afd., Copenhagen, Denmark Relative Quantification of Insulin Gene Expression on the LightCycler Using SYBR Green I 6 Jürgen Loeffler, Medizinische Klinik, Abt. II, Otfried-Müller-Str. 10, 72076 Tübingen, Germany Quantification of T-Cell Receptor Excision Circle DANN Using Fluorescence Resonance Energy Transfer and the LightCycler System 7 Jim Whelan, Plant Molecular Biology Group, University of Western Australia, Crawley, Australia Investigation of Mitochondrial Biogenesis in Plants using Quantitative Real-Time PCR 8 E. Veistinen, Turku University, Dept. Medical Microbiology, Kiinamyllynkatu 13, FIN 20520 Turku Quantification of Ikaros Family Isoforms by Real-Time PCR 9 P. Stordeur, Dep. Immunologie- Hematologie-Transfusion, Hopital Erasme, Brussels, Belgium Methods to quantify cytokine gene expression by Real-Time PCR Oncology 10 Dr. Bernard, Idahotech, Salt Lake City, USA quantitative profiling for breast cancer using DNA and RNA markers 11 Melanie Königshoff, University Giessen Quantification of HER-2/NEU Gene Copy Number in Breast Cancer Tissue 12 Remedios Castelló Cros, Dpto. Bioquímica. Centro de Investigación, Hospital la Fe, Av. Campanar, 21, 46009 Valencia, Spain Quantitative real-time reverse transcription-PCR assay for urokinase plasminogen activator, plasminogen activator inhibitor type 1, and tissue metalloproteinase inhibitor type 1 gene expressions in primary breast cancer 13 C. H. W. Klaasen, C. Wilhelmina Hospital, Dep. Of Med. Microbiology & Infectious Diseases Nijmegen, NL Relative Quantification of Human DNA in Feaces (stool) 14 Chung-Che (Jeff) Chang, Assistant Professor, Director, Hematopathology Fellowship and Molecular/Pharmacogenetics Lab., Dep of Pathology, Medical College of Wisconsin, 9200 W. Wisconsin Ave., Milwaukee, WI 53226 real- time quatification of tumor load (t(14;18))in follicular lymphoma patients 15 P. Bolufer, Laboratorio de Biologia Molecular, Universitario La Fe, Valencia, Spain Real time quantification of AML rearrangements (AML1/ETO and TEL/AML1 ) in the diagnosis and monitoring of acute leukemia Genetics 16 Francisco Barros, INGO, Santiago de Compostela Gene Dosage Determination by Real Time PCR 17 Elaine Lyon, ARUP Laboratories, Salt Lake City, USA deletions and duplications of the cytochrome p450 2D6 gene using a reference gene and competitor (Alison Millson) 18 Karin Berg, Pathology, John Hopkins Medical Inst, Baltimore, USA Analysis of Bone Marrow Engraftment Following in Utero Bone Marrow Transplantation in a Can