Primer Design
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Primer Design
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Author :
language : en
Publisher: Elsevier
Release Date : 2025-07-01
Primer Design written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-07-01 with Science categories.
Primer Design, Volume 57 in the Methods in Microbiology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Methods on discriminating between bacterial strains, Primers to PCR and qPCR techniques, and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in Methods in Microbiology serials - Updated release includes the latest information on Primer Design
Pcr Primer Design
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Author : Anton Yuryev
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Pcr Primer Design written by Anton Yuryev and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
In the past decade, molecular biology has been transformed from the art of cloning a single gene to a statistical science measuring and calculating properties of entire genomes. New high-throughput methods have been developed for genome sequencing and studying the cell at different systematic levels such as transcriptome, proteome, metabolome and other -omes. At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). PCR Primer Design focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, this volume is an exceptional reference for all those involved in studying the genome. In PCR Primer Design, authors describe basic approaches for PCR primer design in addition to specialized methods. These state-of-the-art methods can be used for both genome-scale experiments and for small-scale individual PCR amplifications. This volume will be useful for organizations performing whole genome studies, companies designing instruments that utilize PCR, and individual scientists – geneticists, molecular biologists, molecular geneticists, and more – who routinely use PCR in their research.
Pcr Cloning Protocols
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Author : Bing-Yuan Chen
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
Pcr Cloning Protocols written by Bing-Yuan Chen and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.
PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.
Principles And Technical Aspects Of Pcr Amplification
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Author : Elizabeth van Pelt-Verkuil
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-03-14
Principles And Technical Aspects Of Pcr Amplification written by Elizabeth van Pelt-Verkuil and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-03-14 with Science categories.
Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).
Pyrosequencing Protocols
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Author : Sharon Marsh
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Pyrosequencing Protocols written by Sharon Marsh and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
Pyrosequencing® Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing® technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each protocol with troubleshooting tips specific to that method. Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing® methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing® technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing® comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing®.
Ancient Dna Typing
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Author : Susanne Hummel
language : en
Publisher: Springer Science & Business Media
Release Date : 2003
Ancient Dna Typing written by Susanne Hummel and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003 with Medical categories.
This is the definitive source of information on techniques for the identification and sequencing of old DNA (pieces) and their use in biological and medical research and application. Application of aDNA techniques are useful tools for investigations reaching from evolutionary studies to law enforcement approaches. What brings them together is the interest in specific methods of handling aDNA, i.e. elaborated PCR and sequencing techniques and the interpretation of the results. This books serves as an ideal guideline for it demonstrates how problem-solving strategies can be applied in various areas.
Temperate Horticulture
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Author : Dr. Satish K. Sharma
language : en
Publisher: New India Publishing
Release Date : 2006
Temperate Horticulture written by Dr. Satish K. Sharma and has been published by New India Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with Gardening categories.
Temperate horticulture is a very important component of horticulture as it is only confined to the hilly regions of a country. For fruit crops, it represents a group, which is physiologically diverse from the sub-tropical and tropical fruit crops grown in other regions. For vegetables and floriculture it has immense potential for the keeping the nation well supplied with off-season and exotic vegetables and flowers all the year round. This book, being published to mark the centenary year of the Indian Agricultural Research Institute (1905 - 2005), strives to highlight the status of temperate horticulture in India. Articles on biotechnological and bioinformatics, genetic resources and manipulation, cropping patterns, drip irrigation, hill horticulture and economics of temperate horticulture contributed by scientists from IARI, Dr. YS Parmar University of Horticulture and Forestry, NBPGR, CIPHET, CPRI and various RRS's of IARI, NBPGR and ICAR Research Complex for Eastern and NEH Regions. The book will prove as a complete handbook of temperate horticulture as it will not only serve as a reference material for research workers in horticulture, but will also play a vital role in combating problems and planning future strategies in the field of horticulture.
Phylogenomic Data Acquisition
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Author : W. Bryan Jennings
language : en
Publisher: CRC Press
Release Date : 2016-12-12
Phylogenomic Data Acquisition written by W. Bryan Jennings and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-12-12 with Science categories.
Phylogenomics is a rapidly growing field of study concerned with using genome-wide data—usually in the form of DNA sequence loci—to infer the evolution of genes, genomes, and the Tree of Life. Accordingly, this discipline connects many areas in biology including molecular and genomic evolution, systems biology, molecular systematics, phylogeography, conservation genetics, DNA barcoding, and others. With the advent of Next Generation Sequencing in addition to advances in computer hardware and software over the past decade, researchers can now generate unparalleled phylogenomic datasets that are helping to illuminate many areas in the life sciences. This book is an introduction to the principles and practices of gathering these data. Phylogenomic Data Acquisition: Principles and Practice is intended for a broad cross-section of biologists and anyone else interested in learning how to obtain phylogenomic data using the latest methods.
E Coli Plasmid Vectors
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Author : Nicola Casali
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
E Coli Plasmid Vectors written by Nicola Casali and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.
Handbook Of Neurochemistry And Molecular Neurobiology
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Author : Glen Baker
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-03-26
Handbook Of Neurochemistry And Molecular Neurobiology written by Glen Baker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-03-26 with Medical categories.
The Handbook is intended to be a service to the neuroscience community, to help in finding available and useful information, to point out gaps in our knowledge, and to encourage continued studies. It represents the valuable contributions of the many authors of the chapters and the guidance of the editors and most important, it represents support for research in this discipline. Based on the rapid advances in the years since the second edition