Real Time Nucleic Acid Sensor For Detection Of Salmonella In Sprout Irrigation Water
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Real Time Nucleic Acid Sensor For Detection Of Salmonella In Sprout Irrigation Water
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Author : Andrew Theodore Csordás
language : en
Publisher:
Release Date : 2007
Real Time Nucleic Acid Sensor For Detection Of Salmonella In Sprout Irrigation Water written by Andrew Theodore Csordás and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with categories.
Dna Sensor For Detection Of Salmonella In Alfalfa Sprout Production
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Author : Eric Stefan Jackson
language : en
Publisher:
Release Date : 2002
Dna Sensor For Detection Of Salmonella In Alfalfa Sprout Production written by Eric Stefan Jackson and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with categories.
Rapid Molecular Detection Of Salmonella From Produce Using Real Time Pcr
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Author :
language : en
Publisher:
Release Date : 2009
Rapid Molecular Detection Of Salmonella From Produce Using Real Time Pcr written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with categories.
Recent outbreaks of Salmonella linked to fresh produce emphasize the need for rapid and sensitive assays to help control outbreaks. Reverse-transcriptase PCR (RT-PCR) detects the presence of mRNA (shorter half-life than DNA), with greater potential of detecting viable cells. Rapid real-time methods using fluorescent dyes and probes simultaneously detect and confirm the presence of target nucleic acid, eliminating the need for gel electrophoresis. The objective of this research was to rapidly detect Salmonella Typhimurium from spiked lettuce, tomatoes, and peppers using real-time RT-PCR. Washed and ultraviolet light treated lettuce (~25gram), tomato (~100g), and peppers (~130g) samples were inoculated with high (108 to 106 CFU) and low (103 to 101CFU) Salmonella Typhimurium overnight cultures. Samples were then rinsed or hand massaged with 225 ml 0.05 M glycine-saline buffer containing 0.05% Tween and 3% beef extract. Un-inoculated washed produce and sterile buffer were used as negative controls; with S. Typhimurium as a positive control. RNA was extracted from each sample using the Qiagen RNeasy Mini Kit. RT-PCR was carried out using a SYBR Green I RT-PCR kit with previously described Salmonella specific invA gene primers and an internal amplification control (IAC) to eliminate false negatives. Reaction conditions were RT at 50 degree Celsius/40min; PCR at 95 degree Celsius /45s, 58 degree Celsius /45s, 72 degree Celsius /45s for 40 cycles followed by melt temperature(Tm) analysis in a BioRad iCycler to determine specific invA product (~Tm=87.5 degree Celsius) and IAC (Tm=82 degree Celsius). To improve detection sensitivity of low inocula, spiked lettuce, tomatoes, and peppers were pre-enriched in buffered peptone water for 6 hours at 37 degree Celsius, followed by RNA extraction and RT-PCR detection. Each experiment was repeated twice. Real-time RT-PCR after 6-h pre-enrichment, Qiagen RNA extraction and the SYBR Green I kit gave Salmonella detection up to 103 CFU/25g from lettuce, 104 CFU/~130g from tomatoes, and 104 CFU/25g from peppers. Without enrichment, detection limits were 106 CFU/25g for lettuce, 107 CFU/25g for tomatoes, and 107 CFU/25g for peppers. Sensitive and rapid detection of Salmonella from spiked lettuce, tomatoes, and peppers could still be obtained within one day (~2 working shifts).
Functional Nucleic Acid Based Biosensors For Food Safety Detection
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Author : Yunbo Luo
language : en
Publisher: Springer
Release Date : 2018-07-13
Functional Nucleic Acid Based Biosensors For Food Safety Detection written by Yunbo Luo and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-07-13 with Science categories.
This book highlights the development of a functional nucleic acid based biosensor detection method in the context of food safety. Although there have been major advances in food processing technology in both developed and developing countries, food safety assurance systems are generally becoming more stringent, in response to growing (both real and perceived) food safety problems. These problems are due in part to foodborne microorganisms, heavy metals, and small chemical molecules (biological toxins, pesticide residues, and veterinary drug residues), etc. In addition, the nucleic acid biomarkers (DNA methylation, microRNA, and circRNA) induced by these risk factors are also closely related to food safety. Accordingly, this book offers a brief guide to targets and strategies in functional nucleic acid based biosensors for food safety detection. Divided into several chapters that focus on various respective targets, it will be a valuable resource for students and researchers in the fields of biosensor detection, food science etc.
Sensor Technology For Water Quality Monitoring
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Author : Andrew C. Cannons
language : en
Publisher:
Release Date : 2004-01-01
Sensor Technology For Water Quality Monitoring written by Andrew C. Cannons and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-01-01 with Biosensors categories.
Detection Of Salmonella In Environmental Waters Using Real Time Pcr
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Author : Keerthy Chandrasekar
language : en
Publisher:
Release Date : 2008
Detection Of Salmonella In Environmental Waters Using Real Time Pcr written by Keerthy Chandrasekar and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Enterobacteriaceae categories.
A Solid State Ph Sensor For Rna Detection
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Author :
language : en
Publisher:
Release Date : 2011
A Solid State Ph Sensor For Rna Detection written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with categories.
A Real Time Bacterial Cell Detection System
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Author : Trevor Thomas Pled Barkham
language : en
Publisher:
Release Date : 2009
A Real Time Bacterial Cell Detection System written by Trevor Thomas Pled Barkham and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with Pathogenic bacteria categories.
Acoustic Sensor For Real Time Detection And Quantification Of Pathogens In Water
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Author : Danny Bavli
language : en
Publisher:
Release Date : 2010
Acoustic Sensor For Real Time Detection And Quantification Of Pathogens In Water written by Danny Bavli and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with Pathogenic microorganisms categories.
Development Of Advanced Electrochemical Sensors For Dna Detection At The Point Of Care
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Author : Kuangwen Hsieh
language : en
Publisher:
Release Date : 2012
Development Of Advanced Electrochemical Sensors For Dna Detection At The Point Of Care written by Kuangwen Hsieh and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with categories.
DNA amplification is electrochemically monitored in real time within a monolithic microfluidic device, enabling the detection of as few as 16 copies of Salmonella genomic DNA via a single-step process in under an hour.