Rapid Molecular Detection Of Salmonella From Produce Using Real Time Pcr
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Rapid Molecular Detection Of Salmonella From Produce Using Real Time Pcr
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Author :
language : en
Publisher:
Release Date : 2009
Rapid Molecular Detection Of Salmonella From Produce Using Real Time Pcr written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with categories.
Recent outbreaks of Salmonella linked to fresh produce emphasize the need for rapid and sensitive assays to help control outbreaks. Reverse-transcriptase PCR (RT-PCR) detects the presence of mRNA (shorter half-life than DNA), with greater potential of detecting viable cells. Rapid real-time methods using fluorescent dyes and probes simultaneously detect and confirm the presence of target nucleic acid, eliminating the need for gel electrophoresis. The objective of this research was to rapidly detect Salmonella Typhimurium from spiked lettuce, tomatoes, and peppers using real-time RT-PCR. Washed and ultraviolet light treated lettuce (~25gram), tomato (~100g), and peppers (~130g) samples were inoculated with high (108 to 106 CFU) and low (103 to 101CFU) Salmonella Typhimurium overnight cultures. Samples were then rinsed or hand massaged with 225 ml 0.05 M glycine-saline buffer containing 0.05% Tween and 3% beef extract. Un-inoculated washed produce and sterile buffer were used as negative controls; with S. Typhimurium as a positive control. RNA was extracted from each sample using the Qiagen RNeasy Mini Kit. RT-PCR was carried out using a SYBR Green I RT-PCR kit with previously described Salmonella specific invA gene primers and an internal amplification control (IAC) to eliminate false negatives. Reaction conditions were RT at 50 degree Celsius/40min; PCR at 95 degree Celsius /45s, 58 degree Celsius /45s, 72 degree Celsius /45s for 40 cycles followed by melt temperature(Tm) analysis in a BioRad iCycler to determine specific invA product (~Tm=87.5 degree Celsius) and IAC (Tm=82 degree Celsius). To improve detection sensitivity of low inocula, spiked lettuce, tomatoes, and peppers were pre-enriched in buffered peptone water for 6 hours at 37 degree Celsius, followed by RNA extraction and RT-PCR detection. Each experiment was repeated twice. Real-time RT-PCR after 6-h pre-enrichment, Qiagen RNA extraction and the SYBR Green I kit gave Salmonella detection up to 103 CFU/25g from lettuce, 104 CFU/~130g from tomatoes, and 104 CFU/25g from peppers. Without enrichment, detection limits were 106 CFU/25g for lettuce, 107 CFU/25g for tomatoes, and 107 CFU/25g for peppers. Sensitive and rapid detection of Salmonella from spiked lettuce, tomatoes, and peppers could still be obtained within one day (~2 working shifts).
Detection Of Non Amplified Genomic Dna
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Author : Giuseppe Spoto
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-07-06
Detection Of Non Amplified Genomic Dna written by Giuseppe Spoto and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-07-06 with Science categories.
This book offers an overview of state-of-the-art in non amplified DNA detection methods and provides chemists, biochemists, biotechnologists and material scientists with an introduction to these methods. In fact all these fields have dedicated resources to the problem of nucleic acid detection, each contributing with their own specific methods and concepts. This book will explain the basic principles of the different non amplified DNA detection methods available, highlighting their respective advantages and limitations. Non-amplified DNA detection can be achieved by adopting different techniques. Such techniques have allowed the commercialization of innovative platforms for DNA detection that are expected to break into the DNA diagnostics market. The enhanced sensitivity required for the detection of non amplified genomic DNA has prompted new strategies that can achieve ultrasensitivity by combining specific materials with specific detection tools. Advanced materials play multiple roles in ultrasensitive detection. Optical and electrochemical detection tools are among the most widely investigated to analyze non amplified nucleic acids. Biosensors based on piezoelectric crystal have been also used to detect unamplified genomic DNA. The main scientific topics related to DNA diagnostics are discussed by an outstanding set of authors with proven experience in this field.
Molecular Detection Of Human Bacterial Pathogens
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Author : Dongyou Liu
language : en
Publisher: CRC Press
Release Date : 2011-04-18
Molecular Detection Of Human Bacterial Pathogens written by Dongyou Liu and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-04-18 with Medical categories.
As more original molecular protocols and subsequent modifications are described in the literature, it has become difficult for those not directly involved in the development of these protocols to know which are most appropriate to adopt for accurate identification of bacterial pathogens. Molecular Detection of Human Bacterial Pathogens addresses th
Molecular Detection Of Foodborne Pathogens
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Author : Dongyou Liu
language : en
Publisher: CRC Press
Release Date : 2009-07-28
Molecular Detection Of Foodborne Pathogens written by Dongyou Liu and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-07-28 with Science categories.
While the vast majority of our food supplies are nutritious and safe, foodborne pathogen-related illness still affects millions of people each year. Large outbreaks of foodborne diseases- such as the recent salmonella outbreak linked to various peanut butter products- continue to be reported with alarming frequency.All-Encompassing Guide to Detecti
Principles Of Bacterial Detection Biosensors Recognition Receptors And Microsystems
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Author : Mohammed Zourob
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-09-03
Principles Of Bacterial Detection Biosensors Recognition Receptors And Microsystems written by Mohammed Zourob and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-09-03 with Technology & Engineering categories.
Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems will cover the up-to-date biosensor technologies used for the detection of bacteria. Written by the world's most renowned and learned scientists each in their own area of expertise, Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems is the first title to cover this expanding research field.
Present Knowledge In Food Safety
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Author : Michael E. Knowles
language : en
Publisher: Academic Press
Release Date : 2022-10-08
Present Knowledge In Food Safety written by Michael E. Knowles and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-10-08 with Medical categories.
Present Knowledge in Food Safety: A Risk-Based Approach Through the Food Chain presents approaches for exposure-led risk assessment and the management of changes in the chemical, pathogenic microbiological and physical (radioactivity) contamination of 'food' at all key stages of production, from farm to consumption. This single volume resource introduces scientific advances at all stages of the production to improve reliability, predictability and relevance of food safety assessments for the protection of public health. This book is aimed at a diverse audience, including graduate and post-graduate students in food science, toxicology, microbiology, medicine, public health, and related fields. The book's reach also includes government agencies, industrial scientists, and policymakers involved in food risk analysis. - Includes new technologies such as nanotechnology, genetic modification, and cloning - Provides information on advances in pathogen risk assessment through novel and real-time molecular biological techniques, biomarkers, resistance measurement, and cell-to-cell communication in the gut - Covers the role of the microbiome and the use of surrogates (especially for viruses)
Food Borne Pathogens And Antibiotic Resistance
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Author : Om V. Singh
language : en
Publisher: John Wiley & Sons
Release Date : 2017-01-30
Food Borne Pathogens And Antibiotic Resistance written by Om V. Singh and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-01-30 with Science categories.
Food is an essential means for humans and other animals to acquire the necessary elements needed for survival. However, it is also a transport vehicle for foodborne pathogens, which can pose great threats to human health. Use of antibiotics has been enhanced in the human health system; however, selective pressure among bacteria allows the development for antibiotic resistance. Foodborne Pathogens and Antibiotic Resistance bridges technological gaps, focusing on critical aspects of foodborne pathogen detection and mechanisms regulating antibiotic resistance that are relevant to human health and foodborne illnesses This groundbreaking guide: • Introduces the microbial presence on variety of food items for human and animal consumption. • Provides the detection strategies to screen and identify the variety of food pathogens in addition to reviews the literature. • Provides microbial molecular mechanism of food spoilage along with molecular mechanism of microorganisms acquiring antibiotic resistance in food. • Discusses systems biology of food borne pathogens in terms of detection and food spoilage. • Discusses FDA’s regulations and Hazard Analysis and Critical Control Point (HACCP) towards challenges and possibilities of developing global food safety. Foodborne Pathogens and Antibiotic Resistance is an immensely useful resource for graduate students and researchers in the food science, food microbiology, microbiology, and industrial biotechnology.
Producing Safe Eggs
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Author : Steven Ricke
language : en
Publisher: Academic Press
Release Date : 2016-09-13
Producing Safe Eggs written by Steven Ricke and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-09-13 with Technology & Engineering categories.
Producing Safe Eggs: Microbial Ecology of Salmonella takes the unique approach of interfacing problems of Salmonella and microbial contamination with commercial egg production. It presents in-depth information on microbial contamination, safety and control, physiology, immunology, neurophysiology, and animal welfare, which makes this book a complete reference for anyone involved in the safe production of eggs and egg products in the food industry. This book discusses management and risk factors across the entire egg production process, including practical applications to decrease disease and contaminated food products in poultry houses, processing plants and retail businesses. It is an integral reference for food scientists, food safety and quality professionals, food processors, food production managers, and food business owners, as well as students in food science, safety, microbiology, and animal science. - Includes pre- and post-harvest control measures to reduce microbial contamination and salmonella risks - Presents hot topics regarding vaccination, egg-in-shell pasteurization, and other new technologies currently under development - Provides risk assessment strategies for implementation in business operations - Discusses management and risk factors across the entire egg production process, including practical applications to decrease disease and contaminated food products in poultry houses, processing plants, and retail businesses - Offers a complete reference for anyone involved in the safe production of eggs and egg products in the food industry
New Advances In Identification And Quantification Of Foodborne Pathogens
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Author : Dario de Medici
language : en
Publisher: Frontiers Media SA
Release Date : 2022-01-03
New Advances In Identification And Quantification Of Foodborne Pathogens written by Dario de Medici and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-01-03 with Science categories.
Molecular Diagnostics
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Author : George P. Patrinos
language : en
Publisher: Elsevier
Release Date : 2005-06-06
Molecular Diagnostics written by George P. Patrinos and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-06-06 with Science categories.
Molecular Diagnostics covers current molecular biological techniques used to identify the underlying molecular defects in inherited disease. Although an increasing number of laboratories, both academic and private are moving in that direction, there are only a few books in the existing literature, and they deal only partly with diagnosis at the molecular level. Each chapter includes the principle and a brief description of the technique, followed by exmples from the authors' own epertise. Contributors are well-known experts in their field, and derive from a variety of disciplines, to ensure breadth and depth of coverage. - Examines widely used molecular biology techniques to screen for genetic defects causing inherited disorders - Includes state-of-the-art techniques for the detection of the underlying genetic heterogeneity leading to inherited disorders - Identification of genetically modified organisms (GMO's) - Forensic analysis and every-day issues in a diagnostic laboratory - Discusses ethics, genetic counselling and quality management