Rna Amplification And Analysis

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Rna Amplification And Analysis
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Author : Mekbib Astatke
language : en
Publisher: Springer Nature
Release Date : 2024-06-22
Rna Amplification And Analysis written by Mekbib Astatke and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-06-22 with Science categories.
This volume provides methods to analyze RNA and gain insight to a number of critical cellular functions. Chapters detail RNA isolation and enrichment, RNA amplification and detection, RNA sequencing, analysis of RNA modification, RNA delivery approaches, and analysis of RNA binding and catalytic activity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA Amplifications and Analysis: Methods and Protocols aims to present researchers with cutting-edge technologies with detailed explanations of critical steps, while providing a clear understanding of the overall protocol.
Analysis Of Growth Factor Signaling In Embryos
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Author : Malcolm Whitman
language : en
Publisher: CRC Press
Release Date : 2006-08-15
Analysis Of Growth Factor Signaling In Embryos written by Malcolm Whitman and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-08-15 with Science categories.
Developmental biologists have been driven to investigate growth factor signaling in embryos in order to understand the regulatory mechanisms underlying a given developmental process. Thus, it is critical to explore the technical methods and experimental designs for growth factor signaling in embryos. Focusing on specific pathways or pathway comp
Trends In Rna Research
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Author : Peter A. McNamara
language : en
Publisher: Nova Publishers
Release Date : 2006
Trends In Rna Research written by Peter A. McNamara and has been published by Nova Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with Medical categories.
RNA is a chemical found in the nucleus and cytoplasm of cells. RNA plays an important role in protein synthesis and other chemical activities of the cell. The structure of RNA is similar to that of DNA, although RNA is single stranded whereas DNA has a characteristic "double helix". Genetic information is stored by DNA in the nucleus of cells, and RNA carries that information to other parts of the cell where it is converted into protein. Three types of RNA are; mRNA (messenger RNA, which contains the specific sequence of nucleotides necessary to dictate amino acid sequence in proteins), tRNA (transfer RNA, which serves as the "adapter" to position the appropriate amino acid next to a growing polypeptide chain during protein synthesis), and rRNA (ribosomal RNA, which is the RNA component of ribosomes). This book presents state-of-the-research from throughout the world.
Encyclopedia Of Astrobiology
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Author : Ricardo Amils
language : en
Publisher: Springer
Release Date : 2021-01-14
Encyclopedia Of Astrobiology written by Ricardo Amils and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-01-14 with Science categories.
The interdisciplinary field of Astrobiology constitutes a joint arena where provocative discoveries are coalescing concerning, e.g. the prevalence of exoplanets, the diversity and hardiness of life, and its increasingly likely chances for its emergence. Biologists, astrophysicists, biochemists, geoscientists and space scientists share this exciting mission of revealing the origin and commonality of life in the Universe. The members of the different disciplines are used to their own terminology and technical language. In the interdisciplinary environment many terms either have redundant meanings or are completely unfamiliar to members of other disciplines. The Encyclopedia of Astrobiology serves as the key to a common understanding. Each new or experienced researcher and graduate student in adjacent fields of astrobiology will appreciate this reference work in the quest to understand the big picture. The carefully selected group of active researchers contributing to this work and the expert field editors intend for their contributions, from an internationally comprehensive perspective, to accelerate the interdisciplinary advance of astrobiology.
Single Cell Analysis In Biotechnology And Systems Biology
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Author : Fan-Gang Tseng
language : en
Publisher: MDPI
Release Date : 2018-10-01
Single Cell Analysis In Biotechnology And Systems Biology written by Fan-Gang Tseng and has been published by MDPI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-10-01 with Science categories.
This book is a printed edition of the Special Issue "Single Cell Analysis in Biotechnology and Systems Biology" that was published in IJMS
The Individual Microbe Single Cell Analysis And Agent Based Modelling
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Author : Johan H. J. Leveau
language : en
Publisher: Frontiers Media SA
Release Date : 2019-02-19
The Individual Microbe Single Cell Analysis And Agent Based Modelling written by Johan H. J. Leveau and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-02-19 with categories.
Recent technological advances in single-cell microbiology, using flow cytometry, microfluidics, x-ray fluorescence microprobes, and single-cell -omics, allow for the observation of individuals within populations. Simultaneously, individual-based models (or more generally agent-based models) allow for individual microbes to be simulated. Bridging these techniques forms the foundation of individual-based ecology of microbes (µIBE). µIBE has elucidated genetic and phenotypic heterogeneity that has important consequences for a number of human interests, including antibiotic or biocide resistance, the productivity and stability of industrial fermentations, the efficacy of food preservatives, and the potential of pathogens to cause disease. Individual-based models can help us to understand how these sets of traits of individual microbes influence the above. This eBook compiles all publications from a recent Research Topic in Frontiers in Microbiology. It features recent research where individual observational and/or modelling techniques are applied to gain unique insights into the ecology of microorganisms. The Research Topic “The Individual Microbe: Single-Cell Analysis and Agent-Based Modelling” arose from the 2016 @ASM conference of the same name hosted by the American Society for Microbiology at its headquarters in Washington, D.C. We are grateful to ASM for funding and hosting this conference.
Pcr Protocols
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Author : Michael A. Innis
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Pcr Protocols written by Michael A. Innis and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. - Avoid contamination--with specific instructions on setting up your lab - Avoid cumbersome molecular biological techniques - Discover new applications
Rna Methodologies
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Author : Robert E. Farrell Jr.
language : en
Publisher: Elsevier
Release Date : 2022-11-22
Rna Methodologies written by Robert E. Farrell Jr. and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-11-22 with Science categories.
RNA Methodologies: A Laboratory Guide for Isolation and Characterization, Sixth Edition provides the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on RNA sequencing, RNA in Situ Hybridization, non-coding RNAs, computational RNA biology, transcriptomes and bioinformatics, along with the latest advances in methods and protocols across the field of RNA investigation. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA biology while also giving readers helpful hints and troubleshooting techniques from his own personal experience in this subject area. This book presents the essential knowledge and techniques to use when working with RNA for the experienced practitioner, while also aiding the beginner in fully understanding this important branch of molecular biology. - Presents the latest information covering all aspects of working with RNA, delivering a holistic understanding of this leading field in molecular biology - Builds from basic information on RNA techniques to in-depth protocols for specific applications - Features new chapters on RNA sequencing and RNA in situ hybridization - Includes new material on RNA clinical applications and innovations, including RNA therapeutics and RNA vaccines, with particular relevance to coronavirus - Comprises the latest developments in transcriptomes and bioinformatics, with new material on computational RNA biology, RNA CHiP analysis, aptamer biology and RNA epigenetics
Dna Microarrays
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Author : David Bowtell
language : en
Publisher:
Release Date : 2003
Dna Microarrays written by David Bowtell and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003 with Medical categories.
DNA microarray technology is a new and powerful means to analyze genomes and characterize patterns of gene expression. Its applications are widespread across the many fields of plant and animal biological and biomedical research. This manual, designed to extend and to complement the information in the best–selling Molecular Cloning,is a synthesis of the expertise and experience of more than 30 contributors—all innovators in a fast–moving field. DNA Microarraysprovides authoritative, detailed instruction on the design, construction, and applications of microarrays, as well as comprehensive descriptions of the software tools and strategies required for analysis of images and data.
Serial Analysis Of Gene Expression Sage
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Author : Kåre Lehmann Nielsen
language : en
Publisher: Springer Science & Business Media
Release Date : 2008
Serial Analysis Of Gene Expression Sage written by Kåre Lehmann Nielsen and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Medical categories.
Serial Analysis of Gene Expression (SAGE): Digital Gene Expression Profiling facilitates the introduction of SAGE into the laboratory, and provides a framework for interpreting and comparing data derived from SAGE experiments. Of the several methods of genetic profiling available, only SAGE measures the expression of both known and unknown genes. SAGE studies encompass 50,000 tags and can provide detailed knowledge of the 2000 most highly expressed genes in the tissue sample. The SAGE protocols presented are detailed, fully annotated, and tested, and are all written by experienced SAGE researchers from around the world. Part 1 is dedicated to experimental procedures of SAGE and related methods including aRNA LongSAGE, SuperSAGE, DeepSAGE, and GMAT. Part 2 provides methods for extraction and filtration of tags, analysis of ditag populations, and completing statistically correct comparisons of gene expression profiles. Comparative transcriptomics enables scientists to understand the underlying genetics of biological changes such as development, disease, crop yield, and resistance. SAGE analysis is also used to obtain unknown tags, which can be used as gene-specific primers in Rapid Amplification of cDNA Ends (RACE) reactions to generate full-length transcripts for cloning and sequencing. This book will be an indispensable tool for any lab engaged in genetic profiling and comparative transcriptomics, and will help many laboratories to successfully implement tag-based sequencing methods and procedures and obtain comprehensive, useful, and interpretative data.