Sectioning And Cryosectioning For Electron Microscopy
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Electron Microscopy
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Author : John J. Bozzola
language : en
Publisher: Jones & Bartlett Learning
Release Date : 1999
Electron Microscopy written by John J. Bozzola and has been published by Jones & Bartlett Learning this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with Medical categories.
New edition of an introductory reference that covers all of the important aspects of electron microscopy from a biological perspective, including theory of scanning and transmission; specimen preparation; darkroom, digital imaging, and image analysis; laboratory safety; interpretation of images; and an atlas of ultrastructure. Generously illustrated with bandw line drawings and photographs. Annotation copyrighted by Book News, Inc., Portland, OR
Resin Microscopy And On Section Immunocytochemistry
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Author : Geoffrey R. Newman
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Resin Microscopy And On Section Immunocytochemistry written by Geoffrey R. Newman and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Since antibodies tagged with markers have been developed, immunocytochemistry has become the method of choice for identifying tissue substances or for the localisation of nucleic acid in tissue by in situ hybridisation. Resin-embedded tissue is routinely used and new techniques are constantly introduced. Thus, the novice entering these fields has a breathtaking variety of methods open to him. This labmanual covers the embedding of tissue using epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities and results are discussed so that an understanding of the techniques can be acquired and appropriate choices made. The various resins available and all steps involved in tissue processing, beginning with fixation, as well as the great variety of labelling methods and markers that are commonly used for "on-section" cytochemistry and immunocytochemistry are described, including detailed protocols for the application.
Biomedical Electron Microscopy
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Author : Arvid B. Maunsbach
language : en
Publisher: Elsevier
Release Date : 1998-11-03
Biomedical Electron Microscopy written by Arvid B. Maunsbach and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-11-03 with Science categories.
This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis. - Authored by the key leaders in the biological electron microscopy field - Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines - Introduces students on how to read and interpret electron micrographs
Biomedical Technology And Devices Handbook
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Author : George Zouridakis
language : en
Publisher: CRC Press
Release Date : 2003-08-14
Biomedical Technology And Devices Handbook written by George Zouridakis and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-08-14 with Medical categories.
Concise yet comprehensive, the Biomedical Technology and Devices Handbook illuminates the equipment, devices, and techniques used in modern medicine to diagnose, treat, and monitor human illnesses. With topics ranging from the basic procedures like blood pressure measurement to cutting-edge imaging equipment, biological tests, and genetic engineering, this book is organized to navigate smoothly from simple procedures and concepts to the more sophisticated and complex ones. Each section contains a description of the technique, its technical considerations, and its use according to its applications and relevant body systems. The book includes references to relevant Web sites, protocols, problems, and solutions.
Practical Methods In Electron Microscopy Reid Norma And Beesley Julian E Sectioning And Cryosectioning For Electron Microscopy
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Author : Audry M. Glauert
language : en
Publisher:
Release Date : 1972
Practical Methods In Electron Microscopy Reid Norma And Beesley Julian E Sectioning And Cryosectioning For Electron Microscopy written by Audry M. Glauert and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1972 with Electron microscopy categories.
Low Temperature Microscopy And Analysis
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Author : Patrick Echlin
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Low Temperature Microscopy And Analysis written by Patrick Echlin and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.
The frozen-hydrated specimen is the principal element that unifies the subject of low temperature microscopy, and frozen-hydrated specimens are what this book is all about. Freezing the sample as quickly as possible and then further preparing the specimen for microscopy or microanalysis, whether still embedded in ice or not: there seem to be as many variations on this theme as there are creative scientists with problems of structure and composition to investigate. Yet all share a body of com mon fact and theory upon which their work must be based. Low-Temperature Micros copy and Analysis provides, for the first time, a comprehensive treatment of all the elements to which one needs access. What is the appeal behind the use of frozen-hydrated specimens for biological electron microscopy, and why is it so important that such a book should now have been written? If one cannot observe dynamic events as they are in progress, rapid specimen freezing at least offers the possibility to trap structures, organelles, macro molecules, or ions and other solutes in a form that is identical to what the native structure was like at the moment of trapping. The pursuit of this ideal becomes all the more necessary in electron microscopy because of the enormous increase in resolution that is available with electron-optical instruments, compared to light optical microscopes.
Electron Microscopy Of Model Systems
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Author :
language : en
Publisher: Academic Press
Release Date : 2010-09-24
Electron Microscopy Of Model Systems written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-09-24 with Science categories.
The volume covers the preparation and analysis of model systems for biological electron microscopy. The volume has chapters about prokaryotic as well as eukaryotic systems that are used as so-called model organisms in modern cell biology. These systems include the most popular systems, such as budding and fission yeast, the roundworm C. elegans, the fly Drosophila, zebrafish, mouse, and Arabidopsis, but also organisms that are less frequently used in cell biology, such as Chlamydomonas, Dictyostelium, Trypanosoma, faltworms, Axolotl and others. In addition, tissues and tissue culture systems are also covered. These systems are used for very diverse areas of cell biology, such as cell division, abscission, intracellular transport, cytoskeletal organization, tissue regeneration and others. Moreover, this issue presents the currently most important methods for the preparation of biological specimens. This volume, however, is not a classic EM methods book. The methods are not the main focus of this issue. The main goal here is to cover the methods in the context of the specific requirements of specimen preparation for each model organism or systems. This will be the first compendium covering the various aspects of sample preparation of very diverse biological systems. - Covers the preparation and analysis of model systems for biological electron microscopy - Includes the most popular systems but also organisms that are less frequently used in cell biology - Presents the currently most important methods for the preparation of biological specimens - First compendium covering the various aspects of sample preparation of very diverse biological systems
Cryotechniques In Biological Electron Microscopy
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Author : Rudolf A. Steinbrecht
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Cryotechniques In Biological Electron Microscopy written by Rudolf A. Steinbrecht and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro duced a wealth of successful applications.
Sectioning And Cryosectioning For Electron Microscopy
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Author : Norma Reid
language : en
Publisher:
Release Date : 1991
Sectioning And Cryosectioning For Electron Microscopy written by Norma Reid and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991 with categories.
Electron Microscopy
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Author : John Kuo
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
Electron Microscopy written by John Kuo and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.
In this revised and expanded second edition, Electron Microscopy: Methods and Protocols presents the newest technology in electron microscopy, while maintaining the practicality and accessibility of the acclaimed first edition. Like the first edition, this volume provides clear, concise instructions on processing biological specimens and includes discussion on the underlying principles of the majority of the processes presented. Electron Microscopy comprises two major areas of electron microscopy-transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The TEM area covers several key techniques, including: conventional specimen preparation methods for cultured cells and biomedical and plant tissues; cryospecimen preparation by high-pressure freezing and cryoultramicrotomy negative staining and immunogold labeling techniques; and TEM crystallography and cryo-TEM tomography. The SEM area similarly attends to conventional-, variable pressure-, environmental-, and cryoscanning microscopy techniques, as well as the application of X-ray microanalysis. Protocols for the application of X-ray microanalysis to SEM and mass spectrometry conclude the volume.