Sectioning And Cryosectioning For Electron Microscopy
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Sectioning And Cryosectioning For Electron Microscopy
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Author : Norma Reid
language : en
Publisher:
Release Date : 1991
Sectioning And Cryosectioning For Electron Microscopy written by Norma Reid and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991 with categories.
Practical Methods In Electron Microscopy Sectioning And Cryosectioning For Electron Microscopy
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Author : Audrey M. Glauert
language : en
Publisher:
Release Date : 1972
Practical Methods In Electron Microscopy Sectioning And Cryosectioning For Electron Microscopy written by Audrey M. Glauert and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1972 with Electron microscopy categories.
Practical Methods In Electron Microscopy
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Author : Audrey M. Glauert
language : en
Publisher:
Release Date : 1991
Practical Methods In Electron Microscopy written by Audrey M. Glauert and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991 with Cryobiology categories.
Practical Methods In Electron Microscopy Reid Norma And Beesley Julian E Sectioning And Cryosectioning For Electron Microscopy
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Author : Audry M. Glauert
language : en
Publisher:
Release Date : 1972
Practical Methods In Electron Microscopy Reid Norma And Beesley Julian E Sectioning And Cryosectioning For Electron Microscopy written by Audry M. Glauert and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1972 with Electron microscopy categories.
Practical Methods In Electron Microscopy Sectioning And Cryosectioning For Elctron Microscopy
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Author : Audrey M. Glauert
language : en
Publisher:
Release Date : 1972
Practical Methods In Electron Microscopy Sectioning And Cryosectioning For Elctron Microscopy written by Audrey M. Glauert and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1972 with Electron microscopy categories.
Electron Microscopy Methods And Protocols
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Author : M. A. Nasser Hajibagheri
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Electron Microscopy Methods And Protocols written by M. A. Nasser Hajibagheri and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).
Thin Sectioning And Associated Technics For Electron Microscopy
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Author : Ivan Sorvall Inc
language : en
Publisher:
Release Date : 1959
Thin Sectioning And Associated Technics For Electron Microscopy written by Ivan Sorvall Inc and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1959 with Electron microscopes categories.
Cryotechniques In Biological Electron Microscopy
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Author : Rudolf A. Steinbrecht
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Cryotechniques In Biological Electron Microscopy written by Rudolf A. Steinbrecht and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro duced a wealth of successful applications.
Low Temperature Microscopy And Analysis
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Author : Patrick Echlin
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Low Temperature Microscopy And Analysis written by Patrick Echlin and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.
The frozen-hydrated specimen is the principal element that unifies the subject of low temperature microscopy, and frozen-hydrated specimens are what this book is all about. Freezing the sample as quickly as possible and then further preparing the specimen for microscopy or microanalysis, whether still embedded in ice or not: there seem to be as many variations on this theme as there are creative scientists with problems of structure and composition to investigate. Yet all share a body of com mon fact and theory upon which their work must be based. Low-Temperature Micros copy and Analysis provides, for the first time, a comprehensive treatment of all the elements to which one needs access. What is the appeal behind the use of frozen-hydrated specimens for biological electron microscopy, and why is it so important that such a book should now have been written? If one cannot observe dynamic events as they are in progress, rapid specimen freezing at least offers the possibility to trap structures, organelles, macro molecules, or ions and other solutes in a form that is identical to what the native structure was like at the moment of trapping. The pursuit of this ideal becomes all the more necessary in electron microscopy because of the enormous increase in resolution that is available with electron-optical instruments, compared to light optical microscopes.
Cryopreparation Of Thin Biological Specimens For Electron Microscopy
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Author : Norbert Roos
language : en
Publisher: Oxford University Press, USA
Release Date : 1990
Cryopreparation Of Thin Biological Specimens For Electron Microscopy written by Norbert Roos and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990 with Science categories.
Cryotechniques offer a number of advantages over conventional electron microscopy methods in biology, particularly with regard to preserving the morphology and composition of specimens. This handbook provides a practical guide for researchers, students, and technicians who wish to employ cryotechniques in their work. It covers those methods where the cryofixed specimen is sectioned or otherwise presented as a thin film for examination, giving special attention to topics of current interest. This guide will be of use to workers in a variety of biomedical disciplines, including cell biology, molecular biology, bacteriology, and pathology.