Self Assembly And Fibre Formation Of Elastin Llke Polypeptides
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Self Assembly And Fibre Formation Of Elastin Like Polypeptides
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Author : Judith Tamara Cirulis
language : en
Publisher:
Release Date : 2009
Self Assembly And Fibre Formation Of Elastin Like Polypeptides written by Judith Tamara Cirulis and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with categories.
Elastin is a polymeric protein of the extracellular matrix that imparts the characteristics of extensibility and elastic recoil to tissues. Recombinant polypeptides based on the domain structures and sequences of human elastin self-assemble into organized fibrous structures, with physical properties similar to those of native polymeric elastin. Elastin self-assembly is initiated by a temperature-induced phase separation, called coacervation. Previous to this work, coacervation temperature had been the only parameter available to measure propensity for self-assembly. A variety of techniques were developed using spectrophotometry, microscopy, and rheometry to differentiate the stages of self-assembly, thereby enabling independent observation and quantitation of each stage, and allowing investigations into properties of polypeptides and solution conditions affecting these stages.Self-assembly in the presence of non-elastin, matrix-associated proteins showed that these proteins maintained the coacervate as small droplets, which sometimes flocculated into fibre-like structures. Rheometry demonstrated a second temperature-induced transition above the coacervation temperature, which resulted in gelation and viscoelastic characteristics similar to microgels.Kinetic analysis of self-assembly yielded two additional parameters: coacervation velocity and maturation velocity. Examining the effects of agitation, salt concentration, temperature, polypeptide concentration, size of a polypeptide, hydrophobic domain sequence, and cross-linking domain structure on the kinetics demonstrated that coacervation and maturation are independent stages of self-assembly involving distinct mechanisms. Microscopic observations showed that protein-rich droplets of coacervate grew by coalescence to a stable droplet size, which correlated to differences in maturation velocities between polypeptides. Coacervate droplet growth appeared limited by the formation of organized polypeptide at the surface of the droplets, decreasing surface fluidity. Many of the general principles of the physical chemistry of colloids and emulsions appeared to apply to the formation, growth and stabilization of coacervates of the elastin-like polypeptides.Together, these observations have resulted in a greater level of understanding of the entire self-assembly process, and provided a comprehensive model of elastin-like polypeptide self-assembly that relates to in vivo assembly of elastic fibres.
Self Assembly And Fibre Formation Of Elastin Llke Polypeptides
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Author : Judith Cirulis
language : en
Publisher:
Release Date :
Self Assembly And Fibre Formation Of Elastin Llke Polypeptides written by Judith Cirulis and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.
Self Assembly Of Recombinant Human Elastin Polypeptides With Potential For Use In Biomaterials Applications
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Author : Catherine M. Bellingham
language : en
Publisher:
Release Date : 2001
Self Assembly Of Recombinant Human Elastin Polypeptides With Potential For Use In Biomaterials Applications written by Catherine M. Bellingham and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with categories.
Elastin is an extracellular matrix protein found in a number of tissues, including the large arteries such as the aorta, imparting the characteristics of extensibility and elastic recoil. Once laid down in tissues, polymeric elastin is not subject to turnover but is able to sustain its mechanical resilience through billions of cycles of extension and recoil. The process of ordered assembly of elastin into its extracellular, polymeric form remains one of the least well-understood steps in the biosynthesis of elastin. During this step, side chains of lysine residues in elastin monomers must be oxidatively deaminated and brought into juxtaposition in preparation for crosslinking. ' In vivo', several factors have been proposed to contribute to the alignment of elastin monomers in the formation of polymeric elastin, including a microfibrillar scaffold and a cell surface elastin binding protein. We have used a series of small, recombinant polypeptides based on sequences of human elastin to investigate the roles of various hydrophobic domains in promoting self-aggregation, and to determine whether this self-aggregation facilitates specific alignment of elastin polypeptides allowing crosslink formation at lysine residues. Our results demonstrate that polypeptides with as few as three hydrophobic and two crosslinking domains are able to self-aggregate into fibrillar structures essentially identical in appearance to those formed by the full-length elastin monomer, tropoelastin. Moreover, oxidation of lysine residues following aggregation, using a simple oxidizing agent (pyrroloquinoline quinone), results in spontaneous formation of lysine-derived covalent crosslinks between polypeptides, including desmosine and isodesmosine. Fabrication of these covalently crosslinked elastin polypeptides into membrane structures has also allowed assessment of their physical properties. Such membranes possess an elastic modulus, and extensibility and recoil properties similar to those of native insoluble elastin. These results strongly support the view that, independent of the influences of other factors, monomers of elastin possess an intrinsic ability to organize themselves into polymeric structures, aligning lysine residues for covalent crosslinking and forming matrices with elastomeric properties. Understanding the basis of the self-organizational ability of elastin-based polypeptides may provide important clues for the general design of self-assembling biomaterials.
Peptide Based Materials
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Author : Timothy Deming
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-01-13
Peptide Based Materials written by Timothy Deming and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-01-13 with Technology & Engineering categories.
Synthesis of Polypeptides by Ring-Opening Polymerization of α-Amino Acid N-Carboxyanhydrides, by Jianjun Cheng and Timothy J. Deming.- Peptide Synthesis and Self-Assembly, by S. Maude, L. R. Tai, R. P. W. Davies, B. Liu, S. A. Harris, P. J. Kocienski and A. Aggeli.- Elastomeric Polypeptides, by Mark B. van Eldijk, Christopher L. McGann, Kristi L. Kiick andJan C. M. van Hest.- Self-Assembled Polypeptide and Polypeptide Hybrid Vesicles: From Synthesis to Application, by Uh-Joo Choe, Victor Z. Sun, James-Kevin Y. Tan and Daniel T. Kamei.- Peptide-Based and Polypeptide-Based Hydrogels for Drug Delivery and Tissue Engineering, by Aysegul Altunbas and Darrin J. Pochan.-
Self Assembled Peptide Nanostructures
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Author : Jaime Castillo
language : en
Publisher: CRC Press
Release Date : 2012-11-21
Self Assembled Peptide Nanostructures written by Jaime Castillo and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-11-21 with Medical categories.
The self-organization of bionanostructures into well-defined functional machineries found in nature has been a priceless source of ideas for researchers. The molecules of life, proteins, DNA, RNA, etc., as well as the structures and forms that these molecules assume serve as rich sources of ideas for scientists or engineers who are interested in developing bio-inspired materials for innovations in biomedical fields. In nature, molecular self-assembly is a process by which complex three-dimensional structures with well-defined functions are constructed, starting from simple building blocks such as proteins and peptides. This book introduces readers to the theory and mechanisms of peptide self-assembly processes. The authors present the more common peptide self-assembled building blocks and discuss how researchers from different fields can apply self-assembling principles to bionanotechnology applications. The advantages and challenges are mentioned together with examples that reflect the state of the art of the use of self-assembled peptide building blocks in nanotechnology.
Fuzziness
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Author : Monika Fuxreiter
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-03-07
Fuzziness written by Monika Fuxreiter and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-03-07 with Medical categories.
Detailed characterization of fuzzy interactions will be of central importance for understanding the diverse biological functions of intrinsically disordered proteins in complex eukaryotic signaling networks. In this volume, Peter Tompa and Monika Fuxreiter have assembled a series of papers that address the issue of fuzziness in molecular interactions. These papers provide a broad overview of the phenomenon of fuzziness and provide compelling examples of the central role played by fuzzy interactions in regulation of cellular signaling processes and in viral infectivity. These contributions summarize the current state of knowledge in this new field and will undoubtedly stimulate future research that will further advance our understanding of fuzziness and its role in biomolecular interactions.
Self Assembling Biomaterials
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Author : Helena S. Azevedo
language : en
Publisher: Woodhead Publishing
Release Date : 2018-04-17
Self Assembling Biomaterials written by Helena S. Azevedo and has been published by Woodhead Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-04-17 with Technology & Engineering categories.
Self-assembling biomaterials: molecular design, characterization and application in biology and medicine provides a comprehensive coverage on an emerging area of biomaterials science, spanning from conceptual designs to advanced characterization tools and applications of self-assembling biomaterials, and compiling the recent developments in the field. Molecular self-assembly, the autonomous organization of molecules, is ubiquitous in living organisms and intrinsic to biological structures and function. Not surprisingly, the exciting field of engineering artificial self-assembling biomaterials often finds inspiration in Biology. More important, materials that self-assemble speak the language of life and can be designed to seamlessly integrate with the biological environment, offering unique engineering opportunities in bionanotechnology. The book is divided in five parts, comprising design of molecular building blocks for self-assembly; exclusive features of self-assembling biomaterials; specific methods and techniques to predict, investigate and characterize self-assembly and formed assemblies; different approaches for controlling self-assembly across multiple length scales and the nano/micro/macroscopic properties of biomaterials; diverse range of applications in biomedicine, including drug delivery, theranostics, cell culture and tissue regeneration. Written by researchers working in self-assembling biomaterials, it addresses a specific need within the Biomaterials scientific community. Explores both theoretical and practical aspects of self-assembly in biomaterials Includes a dedicated section on characterization techniques, specific for self-assembling biomaterials Examines the use of dynamic self-assembling biomaterials
Design Principle And Application Of Self Assembled Nanobiomaterials In Biology And Medicine
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Author : Alok Pandya
language : en
Publisher: Academic Press
Release Date : 2022-08-04
Design Principle And Application Of Self Assembled Nanobiomaterials In Biology And Medicine written by Alok Pandya and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-08-04 with Medical categories.
Design, Principle and Application of Self-Assembled Nanobiomaterials in Biology and Medicine discusses recent advances in science and technology using nanoscale units that show the novel concept of combining nanotechnology with various research disciplines within both the biomedical and medicine fields. Self-assembly of molecules, macromolecules, and polymers is a fascinating strategy for the construction of various desired nanofabrication in chemistry, biology, and medicine for advanced applications. It has a number of advantages: (1) It is involving atomic-level modification of molecular structure using bond formation advanced techniques of synthetic chemistry. (2) It draws from the enormous wealth of examples in biology for the development of complex, functional structures. (3) It can incorporate biological structures directly as components in the final systems. (4) It requires that the target self-assembled structures be thermodynamically most stable with relatively defect-free and self-healing. In this book, we cover the various emerging self-assembled nanostructured objects including molecular machines, nano-cars molecular rotors, nanoparticles, nanosheets, nanotubes, nanowires, nano-flakes, nano-cubes, nano-disks, nanorings, DNA origami, transmembrane channels, and vesicles. These self-assembled materials are used for sensing, drug delivery, molecular recognition, tissue engineering energy generation, and molecular tuning. Provides a basic understanding of how to design, and implement various self-assembled nanobiomaterials Covers principles implemented in the constructions of novel nanostructured materials Offers many applications of self-assemblies in fluorescent biological labels, drug and gene delivery, bio-detection of pathogens, detection of proteins, probing of DNA structure, tissue engineering, and many more
Elastomeric Proteins
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Author : Peter R. Shewry
language : en
Publisher: Cambridge University Press
Release Date : 2010-02-04
Elastomeric Proteins written by Peter R. Shewry and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-02-04 with Science categories.
This book was originally published in 2002. Elastic proteins occur in a wide range of biological systems where they have evolved to fulfil precise biological roles. The best known include proteins in vertebrate muscles and connective tissues, such as titin, elastin and fibrillin, and spider silks. However, other examples include byssus and abductin from bivalve molluscs, resilin from arthropods and gluten from wheat. Interest in elastomeric proteins has been high for several reasons. Firstly, their biological and medical significance, particularly in human disease. Secondly, the unusual properties of proteins such as spider silks provide opportunities to develop materials. Thirdly, the development of scanning probe microscopy makes it possible to study structures and biomechanical properties of these proteins at the single molecule level. This book will be of value to anyone with an interest in the various aspects of elastomeric proteins.
Fibrous Assemblies From Synthesis And Nanostructure Characterization To Materials Development And Application
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Author : Cinzia Giannini
language : en
Publisher: Frontiers Media SA
Release Date : 2021-12-01
Fibrous Assemblies From Synthesis And Nanostructure Characterization To Materials Development And Application written by Cinzia Giannini and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-12-01 with Science categories.
