Si Tu Puede
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In Situ Hybridization Protocols
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Author : Ian A. Darby
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
In Situ Hybridization Protocols written by Ian A. Darby and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
The technique of in situ hybridization, in its various forms, has been used routinely in many laboratories for a number of years. In the post-genome era, gene arrays and proteomics have allowed us to identify hitherto unknown unrecognized pathways and mechanisms. However, rather than diminish the importance of in situ hybridization, the now widespread use of screening te- nologies has increased the need to temporally and spatially localize the dist- bution of mRNA expression. Our intention, in In Situ Hybridization Protocols is to provide ample inf- mation for novices planning to set up the in situ hybridization technique and use it in their laboratory for the first time, as well as giving updates of recent developments for those laboratories where in situ hybridization techniques are already in use. Despite its widespread significance, in situ hybridization has retained a re- tation as one of the more difficult and capricious molecular biological te- niques. This may in part be because of the hybrid nature of the technique, which often requires a mixture of molecular biological and histological skills. The two techniques are usually taught and acquired in different streams of biolo- cal science. The step-by-step and detailed protocols provided in In Situ Hybridization Protocols by researchers active in the field should make it p- sible for both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use the technique s- cessfully in their laboratories.
Lasik Laser In Situ Keratomileusis
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Author : Dimitri T. Azar
language : en
Publisher: CRC Press
Release Date : 2002-11-26
Lasik Laser In Situ Keratomileusis written by Dimitri T. Azar and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-11-26 with Medical categories.
LASIK is a timely and authoritative source for refractive, corneal, and cataract surgeons; ophthalmologists; optometrists; ophthalmic surgeons; and upper-level undergraduate, graduate, and medical students in these disciplines. This guide emphasizes the latest tools and step-by-step procedures to increase the safety, predictability, and long-term s
In Situ Rock Stress
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Author : Michael Tucker
language : en
Publisher: CRC Press
Release Date : 2015-10-12
In Situ Rock Stress written by Michael Tucker and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-10-12 with Medical categories.
With the new classification of chronic myeloproliferative disorders, and the rise of interest in molecularly targeted therapies, this timely text brings together international experts on the topic to discuss the current technologies and their implications for the treatment of patients. This title comprehensively covers chronic myeloid leukemia and Ph-negative chronic myeloproliferative disorders and is an essential resource for all practitioners in Hematologic Oncology.
In Situ Mechanics Of Materials
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Author : Pranjal Nautiyal
language : en
Publisher: Springer Nature
Release Date : 2020-07-18
In Situ Mechanics Of Materials written by Pranjal Nautiyal and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-07-18 with Technology & Engineering categories.
This is the first comprehensive book to address in-situ mechanics approach, which relies on real-time imaging during mechanical measurements of materials. The book presents tools, techniques and methods to interrogate the deformation characteristics of a wide array of material classes, and how the mechanics and the material microstructures are correlated. In-situ approach provides unprecedented ability to decipher the mechanical behavior of materials from atomic length scales all the way up to bulk-scale, which is not possible using conventional means. The book also addresses how to capture the deformation behavior of materials under different stress-states and extreme environments. The book will be useful to the new generation of students, scientists and researchers working on the frontiers of material design and innovation as they aim to develop new materials with predictable mechanical properties and technological applications. This book can also serve as a textbook aimed at upper-level undergraduates and graduate-level students who are beginning to delve into the mechanics of materials. Catering to a generation of students that appreciates videos as a didactic tool, this book contains numerous videos to supplement problems, solutions, and case studies.
In Situ Hybridization In Neurobiology
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Author : James H. Eberwine
language : en
Publisher:
Release Date : 1994
In Situ Hybridization In Neurobiology written by James H. Eberwine and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with In situ hybridization categories.
In situ hybridization has become an important and widely used research tool for neurobiologists since the authors' first book on the subject, In Situ Hybridization: Applications to Neurobiology (Oxford, 1987). This new volume examines the methodological advances that have been made in in situhybridization techniques and their impact on the study of the nervous system. These include methods for improving sensitivity, ways of examining multiple mRNAs within the same section, ways of examining both proteins and mRNAs within the same section, and methods for using in situ hybridizationtechnology to examine areas of cellular biological significance, such as translational control. The development of alternative labels to radioactivity for the detection of hybridized probes, and the combined use of in situ hybridization and immunocytochemistry is also covered in this book. Itdiscusses the quantification of in situ hybridization signal and presents an overview of information generated by using in situ hybridization in diagnostic medicine. This volume will be essential to researchers in the neurosciences as well as those in other disciplines.
In Situ Molecular Pathology And Co Expression Analyses
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Author : Gerard J. Nuovo
language : en
Publisher: Academic Press
Release Date : 2020-09-03
In Situ Molecular Pathology And Co Expression Analyses written by Gerard J. Nuovo and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-09-03 with Science categories.
Major advancements in the field of in situ molecular pathology have occurred since publication of the first edition. In Situ Molecular Pathology and Co-expression Analyses, Second Edition, continues to teach both the molecular basis for the improvements and the actual protocols. This is the unique feature that separates it from the pack of other "cook-book" type approaches. The fields of in situ hybridization and immunohistochemistry have expanded rapidly where computer-based analyses systems have greatly expanded the power of these methods. Further, knowledge of the marked improvements in the reagents themselves since the first edition can make the difference of excellent versus misleading data. The automated platforms require that researchers and diagnostic biomedical investigators have a good understanding of the basics of in situ based tests, protocols, and biochemistry for troubleshooting in order to maximize the use of these platforms. This second edition focuses attention on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses. Practicing molecular pathologists, diagnostic pathologists, laboratory directors, and toxicologists, as well as clinicians and researchers in training, will benefit from this clear presentation of protocols and theoretical framework. Data derived from in situ hybridization and immunohistochemistry. Explains the theory and foundation of immunohistochemistry and in situ hybridization and presents easy-to-follow experimental protocols with tricks of the trade Includes two new chapters: Recent improvements in immunohistochemistry and in situ hybridization, Quality control for immunohistochemistry and in situ hybridization: How to know if the color change is signal or background The second edition also includes a detailed test to help one learn the basics of histologic interpretation of tissues and a separate detailed test in how to differentiate signal from background Includes chapter-ending summaries of Key Points to Remember, bringing beginners up to speed with any seasoned veteran in the field Thoughtfully tackles the molecular basis if IHC and ISH, along with application of that knowledge to improving the techniques is significant
In Situ Visualization For Computational Science
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Author : Hank Childs
language : en
Publisher: Springer Nature
Release Date : 2022-05-04
In Situ Visualization For Computational Science written by Hank Childs and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-05-04 with Mathematics categories.
This book provides an overview of the emerging field of in situ visualization, i.e. visualizing simulation data as it is generated. In situ visualization is a processing paradigm in response to recent trends in the development of high-performance computers. It has great promise in its ability to access increased temporal resolution and leverage extensive computational power. However, the paradigm also is widely viewed as limiting when it comes to exploration-oriented use cases. Furthermore, it will require visualization systems to become increasingly complex and constrained in usage. As research efforts on in situ visualization are growing, the state of the art and best practices are rapidly maturing. Specifically, this book contains chapters that reflect state-of-the-art research results and best practices in the area of in situ visualization. Our target audience are researchers and practitioners from the areas of mathematics computational science, high-performance computing, and computer science that work on or with in situ techniques, or desire to do so in future.
In Situ Characterization Techniques For Nanomaterials
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Author : Challa S.S.R. Kumar
language : en
Publisher: Springer
Release Date : 2018-04-17
In Situ Characterization Techniques For Nanomaterials written by Challa S.S.R. Kumar and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-04-17 with Technology & Engineering categories.
Seventh volume of a 40 volume series on nanoscience and nanotechnology, edited by the renowned scientist Challa S.S.R. Kumar. This handbook gives a comprehensive overview about In-situ Characterization Techniques for Nanomaterials. Modern applications and state-of-the-art techniques are covered and make this volume an essential reading for research scientists in academia and industry.
Pcr Rt Pcr In Situ
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Author : Gerard Morel
language : en
Publisher: CRC Press
Release Date : 2002-09-27
Pcr Rt Pcr In Situ written by Gerard Morel and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-09-27 with Science categories.
Although the polymerase chain reaction has revolutionized genetic analysis by amplifying rare nucleic acid sequences, the in situ application is the only method that allows the localization of amplified signal within tissue structure. The applications of in situ polymerase chain reaction have greatly enhanced the field of investigation in many disc
In Situ Burning For Oil Spill Countermeasures
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Author : Merv Fingas
language : en
Publisher: CRC Press
Release Date : 2018-06-13
In Situ Burning For Oil Spill Countermeasures written by Merv Fingas and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-06-13 with Science categories.
Shelving Guide: Environmental Engineering In-situ burning is recognized as a viable alternative for cleaning up oil spills on land and water. It can rapidly reduce the volume of spilled oil and eliminate the need to collect, store, transport, and dispose of recovered oil, and can also shorten the response time to a spill, thus reducing the chances that the spill will spread on the water surface or further into land. This book will serve as a comprehensive reference for all aspects of in-situ burning of oil spills and include the scientific aspects of the burning process and the related effects, as well as practical information about the procedures to be followed and equipment required for carrying out an in-situ burn. Features Serves as a complete source of information on in-situ burning as well as practical guide on how to implement the procedures. Explains procedures for burning in different situations, including on water, land, and ice. Provides information on worker health and safety precautions during burning. Covers several different types of emissions, their environmental fate, and how to monitor them. Includes numerous illustrative case studies.