Understanding The Light Microscope
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Understanding Light Microscopy
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Author : Jeremy Sanderson
language : en
Publisher: John Wiley & Sons
Release Date : 2019-03-04
Understanding Light Microscopy written by Jeremy Sanderson and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-03-04 with Science categories.
Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.
Understanding The Light Microscope
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Author : Dan J. Goldstein
language : en
Publisher: Academic Press
Release Date : 1999-09-03
Understanding The Light Microscope written by Dan J. Goldstein and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-09-03 with Computers categories.
Histology, immunology, histochemistry and microscopy. Since retiring in 1989 as Reader in Anatomy at Sheffield University, he has been an independent research worker in biomedical science. Key Features * Aids insight into microscope operation and imitations * The approach is non-mathematical, yet in-depth * Enables lecture time to be replaced by learning assignments * Includes a help function for all four programs * The programs have been tried and tested by 2nd and 3rd year biomedical undergraduates.
Understanding Light Microscopy
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Author : Jeremy Sanderson
language : en
Publisher: John Wiley & Sons
Release Date : 2019-03-28
Understanding Light Microscopy written by Jeremy Sanderson and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-03-28 with Science categories.
Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.
Introduction To Optical Microscopy
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Author : Jerome Mertz
language : en
Publisher: Cambridge University Press
Release Date : 2019-08
Introduction To Optical Microscopy written by Jerome Mertz and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08 with Medical categories.
Presents a fully updated, self-contained textbook covering the core theory and practice of both classical and modern optical microscopy techniques.
Confocal Microscopy For Biologists
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Author : Alan R. Hibbs
language : en
Publisher: Springer Science & Business Media
Release Date : 2004-04-30
Confocal Microscopy For Biologists written by Alan R. Hibbs and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-04-30 with Science categories.
There has been a great upsurge in interest in light microscopy in recent years due to the advent of a number of significant advances in microscopy, one of the most important of which is confocal microscopy. Confocal microscopy has now become an important research tool, with a large number of new fluorescent dyes becoming available in the past few years, for probing your pet structure or molecule within fixed or living cell or tissue sampies. Many of the people interested in using confocal microscopy to further their research do not have a background in microscopy or even cell biology and so not only do they find considerable difficulty in obtaining satisfactory results with a confocal microscope, but they may be mislead by how data is being presented. This book is intended to teach you the basic concepts ofmicroscopy, fluorescence, digital imaging and the principles of confocal microscopy so that you may take full advantage ofthe excellent confocal microscopes now available. This book is also an excellent reference source for information related to confocal microscopy for both beginners and the more advanced users. For example, do you need to know the optimal pinhole size for a 63x 1. 4 NA lens? Do you need to know the fluorescence emission spectrum of Alexa 568? Access to the wealth of practical information in this book is made easier by using both the detailed index and the extensive glossary.
Introduction To Light Microscopy
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Author : Dee Lawlor
language : en
Publisher: Springer
Release Date : 2019-05-31
Introduction To Light Microscopy written by Dee Lawlor and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-05-31 with Science categories.
This book offers a beginner’s guide to using light microscopes. It begins with a brief introduction to the physics of optics, which will give the reader a basic grasp of the behaviors of light. In turn, each part of the microscope is explained using clear and simple English, together withdetailed photographs and diagrams. The reader will learn the function, care and correct use of each part. A troubleshooting section also helps resolve some of the most common issues encountered in light microscopy. Most people have a general idea of how to use a microscope, but many never get the full benefit, because they receive no training. With easy-to-follow steps and detailed images, this guide will help everyone achieve the best results, and be confident using their microscope. This book is intended for anyone using a light microscope, such as university students, people in lab environments, hobbyists, educators who teach science to young children, and anyonewith a general interest in these valuable tools.
A Practical Guide To Optical Microscopy
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Author : John Girkin
language : en
Publisher: CRC Press
Release Date : 2019-06-14
A Practical Guide To Optical Microscopy written by John Girkin and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-06-14 with Science categories.
Choice Recommended Title, March 2020 Optical microscopy is used in a vast range of applications ranging from materials engineering to in vivo observations and clinical diagnosis, and thanks to the latest advances in technology, there has been a rapid growth in the number of methods available. This book is aimed at providing users with a practical guide to help them select, and then use, the most suitable method for their application. It explores the principles behind the different forms of optical microscopy, without the use of complex maths, to provide an understanding to help the reader utilise a specific method and then interpret the results. Detailed physics is provided in boxed sections, which can be bypassed by the non-specialist. It is an invaluable tool for use within research groups and laboratories in the life and physical sciences, acting as a first source for practical information to guide less experienced users (or those new to a particular methodology) on the range of techniques available. Features: The first book to cover all current optical microscopy methods for practical applications Written to be understood by a non-optical expert with inserts to provide the physical science background Brings together conventional widefield and confocal microscopy, with advanced non-linear and super resolution methods, in one book To learn more about the author please visit here.
Materials Characterization
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Author : Yang Leng
language : en
Publisher: John Wiley & Sons
Release Date : 2008-06-02
Materials Characterization written by Yang Leng and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-06-02 with Science categories.
Part One - Microstructure Examinations Light microscopy X-ray diffraction Transmission electron microscopy Scanning electron microscopy Scanning probe microscopy Part Two--Chemical and Thermal Analysis X-Ray Spectroscopy for Elemental Analysis Electron Spectroscopy for Surface Analysis Secondary Ion Mass Spectrometry for Surface Analysis Vibrational Spectroscopy for Molecular Analysis Thermal analysis.
Applied Polymer Light Microscopy
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Author : Derek A. Hemsley
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Applied Polymer Light Microscopy written by Derek A. Hemsley and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Social Science categories.
Synthetic polymers make excellent specimens for light microscopy. Despite this, the use of the technique, at least in its advanced forms, is not so widespread as might be expected. Although reliable and relevant data are difficult to find and quantify, it seems that in other fields of materials science and technology there is a greater readiness to tum to the microscope in research, in industrial problem solving, or for quality assessment and control. It also seems that the reasons for the present situation are partly historical, partly the result of the structure of the plastics and rubber industries, and partly the education and training background of senior staff who tend to be chemistry or engineering based. In neither field does light microscopy feature strongly in the basic training. The primary aim of this book is to provide some insight into the range oflight microscopy techniques applicable to polymeric specimens, and to highlight typical applications to commercial polymers and polymer products. Where appropriate, the optical techniques involved are discussed in some detail. However, it has not been the intention to produce a light microscopy textbook dealing with the principles and design of the basic instrument. Many such texts are available, and selected examples are cited in the reference list at the end of most chapters.
Light And Electron Microscopy
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Author : Elizabeth M. Slayter
language : en
Publisher: Cambridge University Press
Release Date : 1992-10-30
Light And Electron Microscopy written by Elizabeth M. Slayter and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992-10-30 with Science categories.
The compound optical microscope, in its various modern forms, is probably the most familiar of all laboratory instruments and the electron microscope, once an exotic rarity, has now become a standard tool in biological and materials research. Both instruments are often used effectively with little knowledge of the relevant theory, or even of how a particular type of microscope functions. Eventually however, proper use, interpretation of images and choices of specific applications demand an understanding of fundamental principles. This book describes the principles of operation of each type of microscope currently available and of use to biomedical and materials scientists. It explains the mechanisms of image formation, contrast and its enhancement, accounts for ultimate limits on the size of observable details (resolving power and resolution) and finally provides an account of Fourier optical theory. Principles behind the photographic methods used in microscopy are also described and there is some discussion of image processing methods. The book will appeal to graduate students and researchers in the biomedical sciences, and it will be helpful to students taking a course involving the principles of microscopy.