Advanced Light Microscopy Specialized Methods
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Advanced Light Microscopy Principles And Basic Properties
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Author : Maksymilian Pluta
language : en
Publisher: Pwn
Release Date : 1988
Advanced Light Microscopy Principles And Basic Properties written by Maksymilian Pluta and has been published by Pwn this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with Science categories.
V. 1. Principles and basic properties.
Techniques In Light Microscopy
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Author : Steven E. Ruzin
language : en
Publisher: Oxford University Press
Release Date : 2024-09-27
Techniques In Light Microscopy written by Steven E. Ruzin and has been published by Oxford University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-09-27 with Science categories.
This textbook provides an accessible and pedagogical explanation of the way microscopes magnify images and covers all techniques to date in transmitted and fluorescent light microscopy. The first section covers basic optics as it relates to microscopy. The second section describes all the major optical techniques of transmitted light microscopy, starting with brightfield, through darkfield, polarized light, phase contrast, differential interference contrast, and Hoffman modulation contrast microscopy. The final third of the book covers all the techniques of fluorescence microscopy. It begins with a simple explanation of fluorescence and is followed by Widefield epifluorescence, confocal, and 2-photon microscopy. This is followed by computational imaging including restoration ('deconvolution') microscopy, and all the present super-resolution techniques. The book concludes by discussing attainable resolution using transmitted light microscopy, including a thorough discussion of the Rayleigh, Sparrow, and other criteria of resolution, ending with a short discussion of the common pitfalls that result in decreased microscope resolution. The final chapter in the book is a short history of the microscope, beginning with the ancients, then discussing three seminal natural philosophers: Leeuwenhoek, Swammerdam, and Hooke. The remaining sections of the history chapter cover mechanical and optical advancements in the history of transmitted light microscopy. Finally, it includes a short history of fluorescence microscopy starting with Köhler and Rohr's first use of fluorescence microscopy in 1905, and ending with a description of the Sarastro Phoibos 1000, the first commercial confocal microscope.
Advanced Light Microscopy Specialized Methods
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Author : Maksymilian Pluta
language : en
Publisher:
Release Date : 1988
Advanced Light Microscopy Specialized Methods written by Maksymilian Pluta and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with Microscopy categories.
V. 1. Principles and basic properties.
Fundamentals Of Light Microscopy And Electronic Imaging
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Author : Douglas B. Murphy
language : en
Publisher: John Wiley & Sons
Release Date : 2012-11-05
Fundamentals Of Light Microscopy And Electronic Imaging written by Douglas B. Murphy and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-11-05 with Medical categories.
Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags. The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: www.wiley.com/go/murphy/lightmicroscopy
Advanced Light Microscopy
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Author : Maksymilian Pluta
language : en
Publisher:
Release Date : 1988
Advanced Light Microscopy written by Maksymilian Pluta and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with categories.
Inorganic And Organometallic Polymers With Special Properties
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Author : R.M. Laine
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Inorganic And Organometallic Polymers With Special Properties written by R.M. Laine and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Proceedings of the NATO Advanced Research Workshop, Cap d'Agde, France, September 9-14, 1990
Light And Video Microscopy
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Author : Randy O. Wayne
language : en
Publisher: Academic Press
Release Date : 2019-06-11
Light And Video Microscopy written by Randy O. Wayne and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-06-11 with Technology & Engineering categories.
Light and Video Microscopy, Third Edition provides a step-by-step journey through philosophy, psychology and the geometrical and physical optics involved in interpreting images formed by light microscopes. The book addresses the intricacies necessary to set up light microscopes that allow one to visualize transparent specimens and, in the process, quantitatively determine various physico-chemical properties of specimens. This updated edition includes the most recent developments in microscopy, ensuring that it continues to be the most comprehensive, easy-to-use, and informative guide on light microscopy. With its presentation of geometrical optics, it assists the reader in understanding image formation and light movement within the microscope. - Provides a fully-revised, updated resource on three-dimensional (3D) structures - Contains a new appendices on Diffraction Theory and Advanced Image Processing - Provides practical applications, lab exercises and case studies on the mathematics, physics and biology used in microscopy - Discusses bright field, dark field, phase-contrast, fluorescence, interference, differential interference and modulation contrast microscopes, oblique illumination and photomicrography
Advanced Light Microscopy Measuring Techniques
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Author : Maksymilian Pluta
language : en
Publisher:
Release Date : 1988
Advanced Light Microscopy Measuring Techniques written by Maksymilian Pluta and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with Microscopy categories.
V. 1. Principles and basic properties.
Superresolution Optical Microscopy
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Author : Barry R. Masters
language : en
Publisher: Springer Nature
Release Date : 2020-03-21
Superresolution Optical Microscopy written by Barry R. Masters and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-03-21 with Science categories.
This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes. It takes a critical look at the body of knowledge that comprises optical microscopy, compares and contrasts the various instruments, provides a clear discussion of the physical principles that underpin these techniques, and describes advances in science and medicine for which superresolution microscopes are required and are making major contributions. The text fills significant gaps that exist in other works on superresolution imaging, firstly by placing a new emphasis on the specimen, a critical component of the microscope setup, giving equal importance to the enhancement of both resolution and contrast. Secondly, it covers several topics not typically discussed in depth, such as Bessel and Airy beams, the physics of the spiral phase plate, vortex beams and singular optics, photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM), and light-sheet fluorescence microscopy (LSFM). Several variants of these techniques are critically discussed. Noise, optical aberrations, specimen damage, and artifacts in microscopy are also covered. The importance of validation of superresolution images with electron microscopy is stressed. Additionally, the book includes translations and discussion of seminal papers by Abbe and Helmholtz that proved to be pedagogically relevant as well as historically significant. This book is written for students, researchers, and engineers in the life sciences, medicine, biological engineering, and materials science who plan to work with or already are working with superresolution light microscopes. The volume can serve as a reference for these areas while a selected set of individual chapters can be used as a textbook for a one-semester undergraduate or first-year graduate course on superresolution microscopy. Moreover, the text provides a captivating account of curiosity, skepticism, risk-taking, innovation, and creativity in science and technology. Good scientific practice is emphasized throughout, and the author’s lecture slides on responsible conduct of research are included as an online resource which will be of interest to students, course instructors, and scientists alike.
Coherent Light Microscopy
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Author : Pietro Ferraro
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-02-09
Coherent Light Microscopy written by Pietro Ferraro and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-02-09 with Science categories.
This book deals with the latest achievements in the field of optical coherent microscopy. While many other books exist on microscopy and imaging, this book provides a unique resource dedicated solely to this subject. Similarly, many books describe applications of holography, interferometry and speckle to metrology but do not focus on their use for microscopy. The coherent light microscopy reference provided here does not focus on the experimental mechanics of such techniques but instead is meant to provide a users manual to illustrate the strengths and capabilities of developing techniques. The areas of application of this technique are in biomedicine, medicine, life sciences, nanotechnology and materials sciences.