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Coherent Light Microscopy

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Coherent Light Microscopy

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Author : Pietro Ferraro
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-02-09

Coherent Light Microscopy written by Pietro Ferraro and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-02-09 with Science categories.

This book deals with the latest achievements in the field of optical coherent microscopy. While many other books exist on microscopy and imaging, this book provides a unique resource dedicated solely to this subject. Similarly, many books describe applications of holography, interferometry and speckle to metrology but do not focus on their use for microscopy. The coherent light microscopy reference provided here does not focus on the experimental mechanics of such techniques but instead is meant to provide a users manual to illustrate the strengths and capabilities of developing techniques. The areas of application of this technique are in biomedicine, medicine, life sciences, nanotechnology and materials sciences.

Coherent Light Microscopy

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Author : Pietro Ferraro
language : en
Publisher: Springer
Release Date : 2011-07-20

Coherent Light Microscopy written by Pietro Ferraro and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-07-20 with Technology & Engineering categories.

Fundamentals Of Light Microscopy And Electronic Imaging

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Author : Douglas B. Murphy
language : en
Publisher: John Wiley & Sons
Release Date : 2002-06-18

Fundamentals Of Light Microscopy And Electronic Imaging written by Douglas B. Murphy and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-06-18 with Science categories.

Over the last decade, advances in science and technology have profoundly changed the face of light microscopy. Research scientists need to learn new skills in order to use a modern research microscope-skills such as how to align microscope optics and perform image processing. Fundamentals of Light Microscopy and Electronic Imaging explores the basics of microscope design and use. The comprehensive material discusses the optical principles involved in diffraction and image formation in the light microscope, the basic modes of light microscopy, the components of modern electronic imaging systems, and the image processing operations necessary to acquire and prepare an image. Written in a practical, accessible style, Fundamentals of Light Microscopy and Electronic Imaging reviews such topics as: * Illuminators, filters, and isolation of specific wavelengths * Phase contrast and differential interference contrast * Properties of polarized light and polarization microscopy * Fluorescence and confocal laser scanning microscopy * Digital CCD microscopy and image processing Each chapter includes practical demonstrations and exercises along with a discussion of the relevant material. In addition, a thorough glossary assists with complex terminology and an appendix contains lists of materials, procedures for specimen preparation, and answers to questions. An essential resource for both, experienced and novice microscopists.

Introduction To Optical Microscopy

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Author : Jerome Mertz
language : en
Publisher: Cambridge University Press
Release Date : 2019-08

Introduction To Optical Microscopy written by Jerome Mertz and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08 with Medical categories.

Presents a fully updated, self-contained textbook covering the core theory and practice of both classical and modern optical microscopy techniques.

Understanding Light Microscopy

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Author : Jeremy Sanderson
language : en
Publisher: John Wiley & Sons
Release Date : 2019-03-04

Understanding Light Microscopy written by Jeremy Sanderson and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-03-04 with Science categories.

Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.

Handbook Of Biological Confocal Microscopy

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Author : James Pawley
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Handbook Of Biological Confocal Microscopy written by James Pawley and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.

In 1987 the Electron Microscopy Society of America (EMSA) going to drive important scientific discoveries across wide areas under the leadership of J. P. Revel (Cal Tech) initiated a major of physiology, cellular biology and neurobiology. They had been program to present a discussion of recent advances in light looking for a forum in which they could advance the state of microscopy as part of the annual meeting. The result was three the art of confocal microscopy, alert manufacturers to the lim special LM sessions at the Milwaukee meeting in August 1988: itations of current instruments, and catalyze progress toward The LM Forum, organized by me, and Symposia on Confocal new directions in confocal instrument development. LM, organized by G. Schatten (Madison), and on Integrated These goals were so close to those of the EMSA project that Acoustic/LM/EM organized by C. Rieder (Albany). In addition, the two groups decided to join forces with EMSA to provide there was an optical micro-analysis session emphasizing Raman the organization and the venue for a Confocal Workshop and techniques, organized by the Microbeam Analysis Society, for NSF to provide the financial support for the speakers expenses a total of 40 invited and 30 contributed papers on optical tech and for the publication of extended abstracts.

Superresolution Optical Microscopy

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Author : Barry R. Masters
language : en
Publisher: Springer Nature
Release Date : 2020-03-21

Superresolution Optical Microscopy written by Barry R. Masters and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-03-21 with Science categories.

This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes. It takes a critical look at the body of knowledge that comprises optical microscopy, compares and contrasts the various instruments, provides a clear discussion of the physical principles that underpin these techniques, and describes advances in science and medicine for which superresolution microscopes are required and are making major contributions. The text fills significant gaps that exist in other works on superresolution imaging, firstly by placing a new emphasis on the specimen, a critical component of the microscope setup, giving equal importance to the enhancement of both resolution and contrast. Secondly, it covers several topics not typically discussed in depth, such as Bessel and Airy beams, the physics of the spiral phase plate, vortex beams and singular optics, photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM), and light-sheet fluorescence microscopy (LSFM). Several variants of these techniques are critically discussed. Noise, optical aberrations, specimen damage, and artifacts in microscopy are also covered. The importance of validation of superresolution images with electron microscopy is stressed. Additionally, the book includes translations and discussion of seminal papers by Abbe and Helmholtz that proved to be pedagogically relevant as well as historically significant. This book is written for students, researchers, and engineers in the life sciences, medicine, biological engineering, and materials science who plan to work with or already are working with superresolution light microscopes. The volume can serve as a reference for these areas while a selected set of individual chapters can be used as a textbook for a one-semester undergraduate or first-year graduate course on superresolution microscopy. Moreover, the text provides a captivating account of curiosity, skepticism, risk-taking, innovation, and creativity in science and technology. Good scientific practice is emphasized throughout, and the author’s lecture slides on responsible conduct of research are included as an online resource which will be of interest to students, course instructors, and scientists alike.

Micro And Nano Flow Systems For Bioanalysis

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Author : Michael W. Collins
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-13

Micro And Nano Flow Systems For Bioanalysis written by Michael W. Collins and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-13 with Technology & Engineering categories.

Micro and Nano Flow Systems for Bioanalysis addresses the latest developments in biomedical engineering at very small scales. It shows how organic systems require multi-scale understanding in the broadest sensewhether the approach is experimental or mathematical, and whether the physiological state is healthy or diseased. Micro-and nano-fluidics represent key areas of translational research in which state-of-the-art engineering processes and devices are applied to bedside monitoring and treatment. By applying conventional micro- and nano-engineering to complex organic solids, fluids, and their interactions, leading researchers from throughout the world describe methods and techniques with great potential for use in medicine and clinical practice. Coverage includes the seeming plethora of new, fine-scale optical methods for measuring blood flow as well as endothelial activation and interaction with tissue. Generic areas of modeling and bioelectronics are also considered. In keeping with the recurring theme of medicine and clinical practice, approximately half of the chapters focus on the specific application of micro- and nano- flow systems to the understanding and treatment of cancer and cardiovascular diseases. This book developed from an Expert Overview Session on "Micro & Nano Flows in Medicine: the way ahead" at the 3rd Micro and Nano Flows Conference (MNF2011) held in Thessaloniki, Greece. Additional chapters were included to enhance the international, state-of-the-art coverage.

Confocal Scanning Optical Microscopy And Related Imaging Systems

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Author : Gordon S. Kino
language : en
Publisher: Academic Press
Release Date : 1996-09-18

Confocal Scanning Optical Microscopy And Related Imaging Systems written by Gordon S. Kino and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-09-18 with Science categories.

This book provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineers. The book concentrates mainly on two instruments: the Confocal Scanning Optical Microscope (CSOM), and the Optical Interference Microscope (OIM). A comprehensive discussion of the theory and design of the Near-Field Scanning Optical Microscope (NSOM) is also given. The text discusses the practical aspects of building a confocal scanning optical microscope or optical interference microscope, and the applications of these microscopes to phase imaging, biological imaging, and semiconductor inspection and metrology.A comprehensive theoretical discussion of the depth and transverse resolution is given with emphasis placed on the practical results of the theoretical calculations and how these can be used to help understand the operation of these microscopes. - Provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineers - Explains many practical applications of scanning optical and interference microscopy in such diverse fields as biology and semiconductor metrology - Discusses in theoretical terms the origin of the improved depth and transverse resolution of scanning optical and interference microscopes with emphasis on the practical results of the theoretical calculations - Considers the practical aspects of building a confocal scanning or interference microscope and explores some of the design tradeoffs made for microscopes used in various applications - Discusses the theory and design of near-field optical microscopes - Explains phase imaging in the scanning optical and interference microscopes

Optical Microscopy Of Materials

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Author : Raymond Haynes
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29

Optical Microscopy Of Materials written by Raymond Haynes and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Social Science categories.

Since Sorby published his observations on the structures of steels in 1863, the optical microscope has become one of the most widely used and versatile instruments for examining the structures of engineering materials. Moreover, to examine the diverse range of materials encountered, it must be used in both the reflected-light and transmitted-light forms, and with polarized light. It is complementary to, but not superseded by, the wide range of electron-optical instruments that are now used. Despite its extensive use, it has been described as the most misused, abused, and misunderstood of scientific instruments, for it will produce an image of a sort no matter how badly it is used. To use it effectively, even in its simplest applications, a knowledge of the simple theory of the microscope is necessary, for the theory shows and explains how it should be used. Thus my aim has been to give a simple and, where possible, quantitative account of both the theory and the use of the microscope, including the various special techniques for which it can be used. But, no matter how effectively the microscope is used, if the specimen is inadequately prepared the results of examination will be of doubtful value.

Coherent Light Microscopy

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