Cytoskeleton Methods And Protocols
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Cytoskeleton Methods And Protocols
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Author : Ray H. Gavin
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Cytoskeleton Methods And Protocols written by Ray H. Gavin and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
Over the past two decades experimental studies have solidified the int- pretation of the cytoskeleton as a highly dynamic network of microtubules, actin microfilaments, intermediate filaments, and myosin filaments. Rather than a network of disparate fibers, these polymers are often interconnected and display synergy, which is the combined action of two or more cytoskeletal polymers to achieve a specific cellular structure or function. Cross-commu- cation among cytoskeletal polymers is thought to be achieved through cytoskeletal polymer accessory proteins and molecular motors that bind two or more cytoskeletal polymers. Development of the modern concept of the cytoskeleton is a direct o- growth of advances in experimental tools and reagents that are available to cell and molecular biologists. Technological advances and refinements in cell imaging have made it possible to selectively image a single cytoskeletal po- mer and monitor its dynamics through the use of fluorescence probes in vitro and in vivo. Two decades ago, cytoskeletal research was limited to a few perturbation reagents that included colchicine and cytochalasin. Today, the perturbation arsenal has expanded to a highly selective group of reagents that includes Taxol, nocodazole, benomyl, latrunculin, jasplakinolide, and such endogenous proteins as gelsolin. These reagents enable the investigator to selectively perturb or destroy a cytoskeletal polymer while leaving other cytoskeletal polymers intact. Site-specific monoclonal antibodies that target a specific cytoskeletal polymer have proven to be highly selective affinity tools for cytoskeletal research.
Cytoskeleton
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Author : Ray H. Gavin
language : en
Publisher:
Release Date : 2016
Cytoskeleton written by Ray H. Gavin and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with Anatomy categories.
The third edition of this volume focuses on experimental models that are useful for investigating various aspects of cytoskeleton structure and function. Animal, plant, protist, and fungal models highlight twenty-four chapters that provide detailed protocols for live and fixed-cell imaging, dynamics of cytoskeleton components, cell and organelle motility, and genetics and proteomics. Written in the highly successful Methods in Molecular Biology series format, protocols in each chapter are up-to-date menus organized in a useful step-by-step format appropriate for novice and established investigators. Each chapter is equipped with a valuable notes section that provides a troubleshooting guide and helpful, and often unpublished, technical information aimed at ensuring success with implementation of the protocols. Authoritative and thorough, Cytoskeleton Methods and Protocols, Third Edition helps researchers expand their understanding of cytoskeleton structure and function.
The Plant Cytoskeleton
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Author : Patrick J. Hussey
language : en
Publisher: Springer Nature
Release Date : 2023-02-11
The Plant Cytoskeleton written by Patrick J. Hussey and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-02-11 with Science categories.
This detailed volume explores the development of technologies and protocols that are currently being used to understand the nature and activities of the plant cytoskeleton. A focus for many of the chapters is on sample preparation, as the quality of plant organ/tissue preparation, from single to multicellular samples, determines the quality of the data. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The Plant Cytoskeleton: Methods and Protocols serves as an ideal guide for researchers interested in or starting to be interested in plant cell and molecular biology research.
Methods In Molecular Biology Cytoskeleton Methods And Protocols
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Author : John M. Walker
language : en
Publisher:
Release Date : 1984
Methods In Molecular Biology Cytoskeleton Methods And Protocols written by John M. Walker and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols categories.
Cytoskeleton Dynamics
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Author : Helder Maiato
language : en
Publisher: Humana
Release Date : 2019-12-27
Cytoskeleton Dynamics written by Helder Maiato and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-12-27 with Science categories.
This volume details comprehensive state-of-the-art methods on actin microfilaments and microtubules and how they work to achieve different cellular functions in different cellular contexts. Chapters guide readers through protein purification, in vitro reconstitution of several cytoskeleton properties, analyses of microtubule- and actin-based structures, functional dissection of post-translational modifications, and roles in several biological processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cytoskeleton Dynamics: Methods and Protocols aims to provide a wide range of experimental approaches and be an invaluable resource for present and future generations of cytoskeleton researchers. The chapter “Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Plant Cell Division
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Author : Marie-Cécile Caillaud
language : en
Publisher: Humana
Release Date : 2022-11-11
Plant Cell Division written by Marie-Cécile Caillaud and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-11-11 with Science categories.
This new edition explores innovative approaches and keystone methodologies reflecting the recent advances in the field of plant cell division that have enabled us to study this fascinating process in a quantitative manner, at high resolution both in space and time using cell biology, biochemistry, and molecular biology. After a review of the methods used to visualize the actin cytoskeleton during plant cell division, the book focuses first on methodology to address mitosis progression as a part of the cell cycle. It continues with sections on manipulation of cell division, quantification of cell division patterning, as well as imaging and quantifying plant cytokinesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Plant Cell Division: Methods and Protocols, Second Edition serves as an ideal guide for researchers attempting to visualize, quantify, and modify cell division during cell cycle progression.
Neuronal Cell Death
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Author : Arezu Jahani-Asl
language : en
Publisher: Springer Nature
Release Date : 2022-07-01
Neuronal Cell Death written by Arezu Jahani-Asl and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-07-01 with Medical categories.
This volume covers comprehensive methods on ways to assess structural and ultrastructural changes in the mitochondria, cytoskeleton, and microglia using state-of-the-art microscopy techniques including super-resolution imaging, electron microscopy, and ultra-high field MRI. The chapters in this book cover topics such as analysis of neurodegeneration in the post-mortem characterization of preclinical animal models, in vivo modeling in cell death in different model systems and brain organoids, single cell clonal analysis using Mosaic Analysis with Double Markers in genetic mouse models, and genome and proteomic methods for analysis of mRNA dynamics and quantitation of targeted peptides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Neuronal Cell Death: Methods and Protocols is a valuable resource for any scientist and researcher interested in learning more about this developing field.
The Linc Complex
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Author : Gregg G. Gundersen
language : en
Publisher: Humana
Release Date : 2019-09-07
The Linc Complex written by Gregg G. Gundersen and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-09-07 with Science categories.
This volume describes research methodologies and approaches used to study the Linker of Nucleoskeleton and Cytoskeleton (LINC) complex and its cellular functions. Chapters detail structural and biochemical analysis of LINC complexes, mechanical aspects of the LINC complex, analysis of the LINC complex in model systems and development, and LINC complex in mammalian tissue, organs, and disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The LINC Complex: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Cytoskeleton Signalling And Cell Regulation
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Author : Kermit Carraway
language : en
Publisher: OUP Oxford
Release Date : 1999-12-16
Cytoskeleton Signalling And Cell Regulation written by Kermit Carraway and has been published by OUP Oxford this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-12-16 with Science categories.
This book provides descriptions of experimental methods in research on the cytoskeleton and its relationships to signaling and cell regulation. Thus, it bridges two active and fertile areas of research. The focus is directed particularly towards methods which take advantage of recent advances in molecular biology, microscopy and immunological assays. A second emphasis is on methods for understanding dynamic changes in cells. A third emphasis is on the formation and turnover of macromolecular and supramolecular complexes, which are so important in driving cell regulation and the behaviour of cytoskeletal elements. A combination of practical advice and detailed protocols should make this book valuable for both novice and experienced workers in these burgeoning fields.
Microtubule Dynamics
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Author : Anne Straube
language : en
Publisher: Humana Press
Release Date : 2017-04-30
Microtubule Dynamics written by Anne Straube and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-04-30 with Science categories.
Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and back. In Microtubule Dynamics: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics in vitro and in cells. Beginning with the question of how to analyze microtubule dynamics, the volume continues with detailed descriptions of how to isolate tubulin from different sources and with different posttranslational modifications, methods used to study microtubule dynamics and microtubule interactions in vitro, techniques to investigate the ultrastructure of microtubules and associated proteins, assays to study microtubule nucleation, turnover, and force production in cells, as well as approaches to isolate novel microtubule-associated proteins and their interacting proteins. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Definitive and practical, Microtubule Dynamics: Methods and Protocols provides the key protocols needed by novices and experts on how to perform a broad range of well-established and newly-emerging techniques in this vital field.