Fluorescence Techniques In Cell Biology

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Fluorescence Techniques In Cell Biology

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Author : Andreas A. Thaer
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Fluorescence Techniques In Cell Biology written by Andreas A. Thaer and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.

.For there to be progress in science, there must first be communication between experts of different disciplines. This is particularly true of modern biology which is becoming more and more of an interdisciplinary field. The present situation in cell biology clearly reflects this devel opment and demonstrates that the application of physical techniques was necessary before this field of biological research could be developed on an objective and quantitative basis. The utilization of optical phenomena as measuring parameters at the microscopic level has provided the basis for the development of quantitative cytochemistry. This rapidly growing extension of conventional cytochemistry and histochemistry is based on the visual oberservation of qualitative chemical criteria in correlation with the microscopically resolved structure of cells and tissues. Furthermore, the introduction into cytochemistry of such optical measuring techniques as ab sorption photometry, interferometry, and fluorometry, as well as the measurement of optical anisotropy, diffrac:tion and scattered light, has provided the methodological bridge for the ex change of knowledge between cell biology on the one hand and biochemistry, or molecular biology, on the other.

Fluorescence Techniques In Cell Biology

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Author : Andreas A. Thaer a.o.
language : en
Publisher:
Release Date : 1973

Fluorescence Techniques In Cell Biology written by Andreas A. Thaer a.o. and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1973 with categories.

Noninvasive Techniques In Cell Biology

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Author : J. Kevin Foskett
language : en
Publisher: New York ; Toronto : Wiley-Liss
Release Date : 1990-10-25

Noninvasive Techniques In Cell Biology written by J. Kevin Foskett and has been published by New York ; Toronto : Wiley-Liss this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990-10-25 with Science categories.

An intriguing and informative guide for cell biologists, cytopathologists, physiologists, cellular biochemists and molecular biologists, this volume provides an up-to-date, representative sample of the latest developments in the area of noninvasive measurements of biological processes at the cellular level. Reviews the latest approaches and techniques for probing a variety of dynamic cellular processes with a minimum of structural or chemical invasion. Topics discussed include: design of novel optical probes, emerging technologies for imaging subcellular processes with high temporal and spatial resolution, the application of noninvasive optical techniques to manipulate cells and subcellular organelles within intact cells.

Fluorescence Techniques In Cell Biology

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Author : Manfred Sernetz
language : en
Publisher:
Release Date : 1973

Fluorescence Techniques In Cell Biology written by Manfred Sernetz and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1973 with categories.

Microscopy Techniques

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Author : Jens Rietdorf
language : en
Publisher: Springer Science & Business Media
Release Date : 2005-06-23

Microscopy Techniques written by Jens Rietdorf and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-06-23 with Science categories.

With contributions by numerous experts

Fluorescence Microscopy

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Author : Anda Cornea
language : en
Publisher: Elsevier
Release Date : 2014-02-24

Fluorescence Microscopy written by Anda Cornea and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-02-24 with Science categories.

Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research Offers insight into common challenges in implementing techniques, as well as effective solutions

Biophysical And Biochemical Aspects Of Fluorescence Spectroscopy

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Author : T.G. Dewey
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29

Biophysical And Biochemical Aspects Of Fluorescence Spectroscopy written by T.G. Dewey and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Science categories.

Fluorescence spectroscopy has traditionally found wide application in bio chemistry and cell biology. Since there are relatively few naturally occurring fluorescent biomolecules, fluorescence spectroscopy offers a combination of great specificity and sensitivity. Historically, these features have been ex ploited with great success utilizing both intrinsic and extrinsic probes. Re cent applications have built upon these traditional strengths and have re sulted in the development of new instrumental techniques, novel and convenient fluorescent probes, and a deeper, theoretical understanding of fundamental processes. Frequently, fluorescence techniques are tailored to attack a specific biological problem. These new methods in turn produce new physical situations and phenomena which are often of interest to the physical chemist. Thus, progress in one area stimulates renewed interest in other areas. The goal of this book is to provide detailed monographs on the use of fluorescence to investigate problems at the forefront of biochemistry and cell biology. This book is not meant to be a comprehensive survey but rather to highlight areas of recent developments. It is designed to be readable to the novice and yet provide sufficient detail for the expert to keep abreast of recent developments. The book is organized so that it proceeds from simple biochemical sys tems to more complex cell biological ones. Chapter I on fluorescence quenching of biological structures is a good introductory chapter. It intro duces a number of elementary concepts and discusses applications to pro teins and biomembranes.

Quantitative Imaging In Cell Biology

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Author :
language : en
Publisher: Academic Press
Release Date : 2014-06-25

Quantitative Imaging In Cell Biology written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-06-25 with Science categories.

This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Fluorescence Spectroscopy In Biology

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Author : Martin Hof
language : en
Publisher: Springer Science & Business Media
Release Date : 2004-12-08

Fluorescence Spectroscopy In Biology written by Martin Hof and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-12-08 with Science categories.

Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.

Fluorescence Microscopy And Fluorescent Probes

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Author : J. Slavík
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11

Fluorescence Microscopy And Fluorescent Probes written by J. Slavík and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.

Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.