Gene Isolation And Mapping Protocols Methods In Molecular Biology
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Gene Isolation And Mapping Protocols
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Author : Jacqueline Boultwood
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Gene Isolation And Mapping Protocols written by Jacqueline Boultwood and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
An unprecedented collection of all the most up-to-date techniques for gene isolation and mapping, including the latest methods for gene characterization using database analyses. This collection of thoroughly tested recipes also includes chapters for the computational analysis of novel cDNA sequences with up-to-the-minute information on basic sequence analysis, sequence similarity searches, exon detection and similarity searches, and the prediction of gene function. Its state-of-the-art methods constitute indispensable tools for all scientists engaged in the search for specific disease genes, or in the general advancement of the human genome project.
Gene Isolation And Mapping Protocols Methods In Molecular Biology
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Author : Jacqueline Boultwood
language : en
Publisher:
Release Date : 1997
Gene Isolation And Mapping Protocols Methods In Molecular Biology written by Jacqueline Boultwood and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with categories.
An unprecedented collection of all the most up-to-date techniques for gene isolation and mapping, including the latest methods for gene characterization using database analyses. This collection of thoroughly tested recipes also includes chapters for the computational analysis of novel cDNA sequences with up-to-the-minute information on basic sequence analysis, sequence similarity searches, exon detection and similarity searches, and the prediction of gene function. Its state-of-the-art methods constitute indispensable tools for all scientists engaged in the search for specific disease genes, or in the general advancement of the human genome project.
Methods In Molecular Biology Gene Isolation And Mapping Protocols
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Author : John M. Walker
language : en
Publisher:
Release Date : 1984
Methods In Molecular Biology Gene Isolation And Mapping Protocols written by John M. Walker and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols categories.
Forensic Dna Profiling Protocols
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Author : Patrick J. Lincoln
language : en
Publisher: Springer Science & Business Media
Release Date : 1998-01-22
Forensic Dna Profiling Protocols written by Patrick J. Lincoln and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-01-22 with Law categories.
This state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The methods include the recovery of DNA from a large range of sample types, analysis of DNA as single and multi-locus VNTR probes, PCR amplification of STR and other loci, and mitochondrial sequencing. The expert scientists writing here -- many from laboratories around the world -- also discuss how to interpret the results in cases of unknown identity and disputed parentage.-- Covers all steps from extraction of human DNA through to analysis and interpretation-- Takes advantage of new methodologies such as capillary electrophoresis-- Clear step-by-step instructions ensure unfailing reproducibility.
Integrin Protocols
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Author : Anthony R. Howlett
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Integrin Protocols written by Anthony R. Howlett and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
Immunochemical Protocols
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Author : John Pound
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Immunochemical Protocols written by John Pound and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
This much anticipated second edition provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for molecular biologists. It covers the breadth of relevant established methods from protein blotting and immunoassays through to visualization of cellular antigens and in situ hybridization, each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including "conventional" and recombinant antibodies, fusion proteins and their various conjugates. This book will open the door to a new generation of immunochemical reagents with exciting possibilities.
Glycoanalysis Protocols
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Author : Elizabeth F. Hounsell
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Glycoanalysis Protocols written by Elizabeth F. Hounsell and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
Glycoanalysis Protocols, Second Edition, makes available to all protein scientists, and particularly those working in today's pharmaceuticals industry, the most advanced and reproducible glycoanalysis techniques. These detailed, up-to-date, and proven analytical methods cover the areas of glycoprotein macromolecular structural analysis, oligosaccharide profiling, lipid conjugate characterization, microorganism structure determination, and proteoglycan function. They also include advanced analytical techniques in biotechnology during the production of recombinant glycoproteins and other therapeutics. These protocols will well serve anyone starting work on the analysis of glycoproteins, as well as experienced investigators seeking to carry their expertise to higher levels of accomplishment.
Basic Cell Culture Protocols
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Author : Jeffrey W. Pollard
language : en
Publisher: Springer Science & Business Media
Release Date : 1997
Basic Cell Culture Protocols written by Jeffrey W. Pollard and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Medical categories.
Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, 2nd ed. offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. Like the first edition, this book should be in every cell culture laboratory and be of use to all who use cell cultures in research.
Protein Targeting Protocols
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Author : Roger A. Clegg
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Protein Targeting Protocols written by Roger A. Clegg and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
It is by no means a revelation that proteins are not uniformly distributed throughout the cell. As a result, the idea that protein molecules, because of the specificity with which they can engage in interactions with other proteins, may be aimed—via these interactions—at a restricted target, is a fundamental one in contemporary molecular life sciences. The target may be variously c- ceived as a specific molecule, a group of molecules, a structure, or a more generic type of intracellular environment. Because the concept of protein targeting is intuitive rather than expl- itly defined, it has been variously used by different groups of researchers in cell biology, biochemistry, and molecular biology. For those working in the field of intracellular signaling, an influential introduction to the topic was the seminal article by Hubbard & Cohen (TIBS [1993] 18, 172–177), which was based on the work of Cohen’s laboratory on protein phosphatases. Sub- quently, the ideas that they discussed have been further developed and extended by many workers to other key intermediaries in intracellular sign- ing, including protein kinases and a great variety of modulator and adaptor proteins.
Plant Virology Protocols
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Author : Gary D. Foster
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Plant Virology Protocols written by Gary D. Foster and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
The aim of Plant Virology Protocols is to provide a source of infor- tion to guide the reader through the wide range of methods involved in gen- ating transgenic plants that are resistant to plant viruses. To this end, we have commissioned a wide-ranging list of chapters that will cover the methods required for: plant virus isolation; RNA extraction; cloning coat p- tein genes; introduction of the coat protein gene into the plant genome; and testing transgenic plants for resistance. The book then moves on to treatments of the mechanisms of resistance, the problems encountered with field testing, and key ethical issues surrounding transgenic technology. Although Plant Virology Protocols deals with the cloning and expression of the coat protein gene, the techniques described can be equally applied to other viral genes and nucleotide sequences, many of which have also been shown to afford protection when introduced into plants. The coat protein has, however, been the most widely applied, and as such has been selected to illustrate the techniques involved. Plant Virology Protocols has been divided into six major sections, c- taining 55 chapters in total.