Methods In Molecular Biology Gene Isolation And Mapping Protocols

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Gene Isolation And Mapping Protocols

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Author : Jacqueline Boultwood
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02

Gene Isolation And Mapping Protocols written by Jacqueline Boultwood and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.

An unprecedented collection of all the most up-to-date techniques for gene isolation and mapping, including the latest methods for gene characterization using database analyses. This collection of thoroughly tested recipes also includes chapters for the computational analysis of novel cDNA sequences with up-to-the-minute information on basic sequence analysis, sequence similarity searches, exon detection and similarity searches, and the prediction of gene function. Its state-of-the-art methods constitute indispensable tools for all scientists engaged in the search for specific disease genes, or in the general advancement of the human genome project.

Gene Isolation And Mapping Protocols Methods In Molecular Biology

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Author : Jacqueline Boultwood
language : en
Publisher:
Release Date : 1997

Gene Isolation And Mapping Protocols Methods In Molecular Biology written by Jacqueline Boultwood and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with categories.

An unprecedented collection of all the most up-to-date techniques for gene isolation and mapping, including the latest methods for gene characterization using database analyses. This collection of thoroughly tested recipes also includes chapters for the computational analysis of novel cDNA sequences with up-to-the-minute information on basic sequence analysis, sequence similarity searches, exon detection and similarity searches, and the prediction of gene function. Its state-of-the-art methods constitute indispensable tools for all scientists engaged in the search for specific disease genes, or in the general advancement of the human genome project.

Methods In Molecular Biology Gene Isolation And Mapping Protocols

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Author : John M. Walker
language : en
Publisher:
Release Date : 1984

Methods In Molecular Biology Gene Isolation And Mapping Protocols written by John M. Walker and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols categories.

Integrin Protocols

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Author : Anthony R. Howlett
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03

Integrin Protocols written by Anthony R. Howlett and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.

Glycoanalysis Protocols

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Author : Elizabeth F. Hounsell
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02

Glycoanalysis Protocols written by Elizabeth F. Hounsell and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.

Glycoanalysis Protocols, Second Edition, makes available to all protein scientists, and particularly those working in today's pharmaceuticals industry, the most advanced and reproducible glycoanalysis techniques. These detailed, up-to-date, and proven analytical methods cover the areas of glycoprotein macromolecular structural analysis, oligosaccharide profiling, lipid conjugate characterization, microorganism structure determination, and proteoglycan function. They also include advanced analytical techniques in biotechnology during the production of recombinant glycoproteins and other therapeutics. These protocols will well serve anyone starting work on the analysis of glycoproteins, as well as experienced investigators seeking to carry their expertise to higher levels of accomplishment.

Protein Targeting Protocols

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Author : Roger A. Clegg
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04

Protein Targeting Protocols written by Roger A. Clegg and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.

It is by no means a revelation that proteins are not uniformly distributed throughout the cell. As a result, the idea that protein molecules, because of the specificity with which they can engage in interactions with other proteins, may be aimed—via these interactions—at a restricted target, is a fundamental one in contemporary molecular life sciences. The target may be variously c- ceived as a specific molecule, a group of molecules, a structure, or a more generic type of intracellular environment. Because the concept of protein targeting is intuitive rather than expl- itly defined, it has been variously used by different groups of researchers in cell biology, biochemistry, and molecular biology. For those working in the field of intracellular signaling, an influential introduction to the topic was the seminal article by Hubbard & Cohen (TIBS [1993] 18, 172–177), which was based on the work of Cohen’s laboratory on protein phosphatases. Sub- quently, the ideas that they discussed have been further developed and extended by many workers to other key intermediaries in intracellular sign- ing, including protein kinases and a great variety of modulator and adaptor proteins.

Plant Virology Protocols

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Author : Gary D. Foster
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03

Plant Virology Protocols written by Gary D. Foster and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.

The aim of Plant Virology Protocols is to provide a source of infor- tion to guide the reader through the wide range of methods involved in gen- ating transgenic plants that are resistant to plant viruses. To this end, we have commissioned a wide-ranging list of chapters that will cover the methods required for: plant virus isolation; RNA extraction; cloning coat p- tein genes; introduction of the coat protein gene into the plant genome; and testing transgenic plants for resistance. The book then moves on to treatments of the mechanisms of resistance, the problems encountered with field testing, and key ethical issues surrounding transgenic technology. Although Plant Virology Protocols deals with the cloning and expression of the coat protein gene, the techniques described can be equally applied to other viral genes and nucleotide sequences, many of which have also been shown to afford protection when introduced into plants. The coat protein has, however, been the most widely applied, and as such has been selected to illustrate the techniques involved. Plant Virology Protocols has been divided into six major sections, c- taining 55 chapters in total.

Electron Microscopy Methods And Protocols

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Author : M. A. Nasser Hajibagheri
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02

Electron Microscopy Methods And Protocols written by M. A. Nasser Hajibagheri and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.

Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).

Receptor Binding Techniques

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Author : Mary Keen
language : en
Publisher: Springer Science & Business Media
Release Date : 1999

Receptor Binding Techniques written by Mary Keen and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with Medical categories.

This cutting-edge collection of step-by-step experimental protocols demonstrates

Using The Biological Literature

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Author : Diane Schmidt
language : en
Publisher: CRC Press
Release Date : 2001-12-06

Using The Biological Literature written by Diane Schmidt and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-12-06 with Language Arts & Disciplines categories.

"Provides an in-depth review of current print and electronic tools for research in numerous disciplines of biology, including dictionaries and encyclopedias, method guides, handbooks, on-line directories, and periodicals. Directs readers to an associated Web page that maintains the URLs and annotations of all major Inernet resources discussed in th