Gene Targeting And Insertional Mutagenesis In Embryonic Stem Cells
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Gene Targeting And Insertional Mutagenesis In Embryonic Stem Cells
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Author : Helen Fiona Sutherland
language : en
Publisher:
Release Date : 1992
Gene Targeting And Insertional Mutagenesis In Embryonic Stem Cells written by Helen Fiona Sutherland and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with Genes, Homeo box categories.
Gene Targeting And Insertional Mutagenesis In Embryonic Stem Cells
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Author : Helen Fiona Sutherland
language : en
Publisher:
Release Date : 1991
Gene Targeting And Insertional Mutagenesis In Embryonic Stem Cells written by Helen Fiona Sutherland and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991 with Genes, Homeo box categories.
Gene Targeting And Embryonic Stem Cells
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Author : Jim Mcwhir
language : en
Publisher: Garland Science
Release Date : 2004-03-01
Gene Targeting And Embryonic Stem Cells written by Jim Mcwhir and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-03-01 with Science categories.
Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference. The recent isolation of human embryonic stem cells and the potential of these cells for therapeutic applications has generated an entirely new methodology. Similarly, gene targeting methodology has recently been extended to nuclear donor cells in ungulate species. This volume will be invaluable for both new and established researchers in the field of human embryonic stem cells, and to biotech companies engaged in the production of transgenic proteins in livestock, xenotransplantation and the development of animal models.
Gene Knockout Protocols
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Author : Martin J. Tymms
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Gene Knockout Protocols written by Martin J. Tymms and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Medical categories.
As the major task of sequencing the human genome is near completion and full complement of human genes are catalogued, attention will be focused on the ultimate goal: to understand the normal biological functions of these genes, and how alterations lead to disease states. In this task there is a severe limitation in working with human material, but the mouse has been adopted as the favored animal model because of the available genetic resources and the highly conserved gene conservation linkage organization. In just of ten years since the first gene-targeting experiments were p- formed in embryonic stem (ES) cells and mutations transmitted through the mouse germline, more than a thousand mouse strains have been created. These achievements have been made possible by pioneering work that showed that ES cells derived from preimplantation mouse embryos could be cultured for prolonged periods without differentiation in culture, and that homologous rec- bination between targeting constructs and endogenous DNA occurred at a f- quency sufficient for recombinants to be isolated. In the next few years the mouse genome will be systematically altered, and the techniques for achi- ing manipulations are constantly being streamlined and improved.
Insertional Mutagenesis Strategies In Cancer Genetics
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Author : Adam J. Dupuy
language : en
Publisher: Springer Science & Business Media
Release Date : 2010-11-18
Insertional Mutagenesis Strategies In Cancer Genetics written by Adam J. Dupuy and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-11-18 with Medical categories.
The goal of this work is summarize the contribution that insertional mutagenesis has made to our understanding of cancer. A variety of insertional mutagens are presented that have been used to study a variety of tumor types in several model organisms. In addition, the impact of insertional mutagenesis in several gene therapy trials is discussed along with strategies to avoid such complications in future clinical trials.
Gene Targeting
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Author : Alexandra L. Joyner
language : en
Publisher: Oxford University Press, USA
Release Date : 1993
Gene Targeting written by Alexandra L. Joyner and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with Language Arts & Disciplines categories.
An introduction for beginners and a bench reference generally to the techniques now available by which scientists can create essentially any mutation in the germline of mice by using recombination and embryonic stem cells (ESC). Genetic researchers, most from the US but also representing Britain, Canada, and Germany, cover principles and practice in mammalian cells, site-specific recombination in cells and mice, producing targeted ESC clones, producing chimeras by blastocyst and morula injection of targeted ESC, producing and analyzing ESC aggregation, gene trap strategies, and classical genetics and gene targeting. The appendix lists suppliers and contact information but not products. Annotation copyrighted by Book News, Inc., Portland, OR
Site Directed Insertion Of Transgenes
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Author : Sylvaine Renault
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-07-30
Site Directed Insertion Of Transgenes written by Sylvaine Renault and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-07-30 with Science categories.
The post-genomic era has brought new challenges and opportunities in all fields of the biology. In this context, several genome engineering technologies have emerged that will help deciphering genes function by as well as improve gene therapy strategies. Genomic modifications such as knock-in, knock-out, knock-down, sequence replacement or modification can today be routinely performed. However, in front of this large palette of methodologies scientists may experience difficulties to gather useful information’s scattered within the literature. This book aims to present the state of this field from basic mechanisms of site-directed modifications to their applications in a wide range of organisms such as bacteria, yeast, plants, insects, mammals. It will discuss the problems encountered when using the random integration strategy and present the recent advances made in targeted genome modification. Technologies based on Zinc Finger nucleases, Meganucleases, TALEN, CRE and FLP recombinase, C31 integrase, transposases and resolvases are fully detailed with their strengths and weaknesses. All these information’s will help students and experienced researchers to understand and choose the best technology for their own purposes.
Targeted Genome Editing Using Site Specific Nucleases
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Author : Takashi Yamamoto
language : en
Publisher: Springer
Release Date : 2015-01-05
Targeted Genome Editing Using Site Specific Nucleases written by Takashi Yamamoto and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-01-05 with Science categories.
This book serves as an introduction to targeted genome editing, beginning with the background of this rapidly developing field and methods for generation of engineered nucleases. Applications of genome editing tools are then described in detail, in iPS cells and diverse organisms such as mice, rats, marine invertebrates, fish, frogs, and plants. Tools that are mentioned include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, all of which have received much attention in recent years as breakthrough technologies. Genome editing with engineered nucleases allows us to precisely change the target genome of living cells and is a powerful way to control functional genes. It is feasible in almost all organisms ranging from bacteria to plants and animals, as well as in cultured cells such as ES and iPS cells. Various genome modifications have proven successful, including gene knockout and knock-in experiments with targeting vectors and chromosomal editing. Genome editing technologies hold great promise for the future, for example in biomedical research, clinical medicine, and generation of crops and livestock with desirable traits. A wide range of readers will find this book interesting, and with its focus on applications in a variety of organisms and cells, the book will be valuable for life scientists in all fields.
Gene Targeting In Embryonic Stem Cells And The Manipulation Of Milk Composition
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Author : Satish Kumar
language : en
Publisher:
Release Date : 1994
Gene Targeting In Embryonic Stem Cells And The Manipulation Of Milk Composition written by Satish Kumar and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Genetic engineering categories.
Genome Editing
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Author : Kursad Turksen
language : en
Publisher: Springer
Release Date : 2016-08-09
Genome Editing written by Kursad Turksen and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-09 with Science categories.
This timely volume explores the use of CRISPR-Cas9 for genome editing, presenting cutting-edge techniques and their applications in treatment of disease. The chapters describe latest methods such as use of targetable nucleases, investigation of the non-coding genome, mouse genome editing, increasing of knock-in efficiency in mouse zygotes, and generation of reporter stem cells; the text contextualizes these methods in treatment of cardiovascular disease, diabetes mellitus, retinitis pigmentosa, and others. The final chapters round out the book with a discussion of controversies and future directions. Genome Editing is an essential, of-the-moment contribution to this rapidly growing field. Drawing from a wealth of international perspectives, it presents novel techniques and applications for the engineering of the human genome. This book is essential reading for all clinicians and researchers in stem cells, regenerative medicine, genomics, biochemical and biomedical engineering- especially those interested in learning more about genome editing and applying it in a targeted, specific way.