Immunochemical Protocols Methods In Molecular Biology
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Immunochemical Protocols
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Author : John Pound
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Immunochemical Protocols written by John Pound and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
This much anticipated second edition provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for molecular biologists. It covers the breadth of relevant established methods from protein blotting and immunoassays through to visualization of cellular antigens and in situ hybridization, each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including "conventional" and recombinant antibodies, fusion proteins and their various conjugates. This book will open the door to a new generation of immunochemical reagents with exciting possibilities.
Immunochemical Protocols Methods In Molecular Biology
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Author : John Pound
language : en
Publisher:
Release Date : 1998
Immunochemical Protocols Methods In Molecular Biology written by John Pound and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with categories.
This much anticipated second edition of the best selling Immunological Protocols provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for ready use by molecular biologists. It covers the breadth of relevant established methods-from protein blotting and immunoassays through the visualization of cellular antigens and in situ hybridization-each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including conventional" and recombinant antibodies, fusion proteins, and their various conjugates. The second edition of Immunochemical Protocols brings many previously specialized techniques within the grasp of all laboratory workers. It remains, like its predecessor, "an excellent laboratory manual and reference" and opens the door to the productive use of a new generation of immunochemical reagents with exciting possibilities."
Immunochemical Methods In Cell And Molecular Biology
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Author :
language : en
Publisher: Academic Press
Release Date : 1987
Immunochemical Methods In Cell And Molecular Biology written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987 with Science categories.
This book presents up-to-date immunochemical techniques for isolating and analyzing a wide variety of biological products. It collates existing information in one source, presenting important basic and applied advances and provides a stimulating forum for the discussion of new ideas and observations on genetically manipulated microbial and viral agents.
Proteomic Profiling
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Author : Anton Posch
language : en
Publisher:
Release Date : 2021
Proteomic Profiling written by Anton Posch and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021 with Cytology categories.
Antibody Methods And Protocols
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Author : Gabriele Proetzel
language : en
Publisher: Humana Press
Release Date : 2012-06-24
Antibody Methods And Protocols written by Gabriele Proetzel and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-06-24 with Medical categories.
The rapidly growing field of antibody research is the result of many advancing technologies allowing current developments to take advantage of molecular engineering to create tailor-made antibodies. Antibody Methods and Protocols attempts to provide insight into the generation of antibodies using in vitro and in vivo approaches, as well as technical aspects for screening, analysis, and modification of antibodies and antibody fragments. The detailed volume is focused on basic protocols for isolating antibodies and, at the same time, it selects a range of specific areas with the aim of providing guides for the overall process of antibody isolation and characterization as well as protocols for enhancing classical antibodies and antibody fragments. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Antibody Methods and Protocols provides a broad and useful background to support ongoing efforts by novices and experts alike and encourages the development of new imaginative approaches to this vital area of study.
Immunohistochemistry And Immunocytochemistry
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Author : Luis Del Valle
language : en
Publisher:
Release Date : 2022
Immunohistochemistry And Immunocytochemistry written by Luis Del Valle and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022 with Electronic books categories.
This volume provides a comprehensive reference guide for researchers to study the applications of labeled antibodies. Chapters guide reader through the the and practice of immunohistochemistry, immunocytochemistry and immunofluorescence techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and useful tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Immunohistochemistry and Immunofluorescence: Methods and Protocols aims to be a useful practical guide to scientists to help further their study in this field.
Immunocytochemical Methods And Protocols
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Author : Lorette C. Javois
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Immunocytochemical Methods And Protocols written by Lorette C. Javois and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Medical categories.
The principle that antibodies can be used as cytochemical agents provided they are tagged with suitable markers has been evident for over 50 years. During this time the use of immunocytochemical meth ods has spread to a wide array of biological disciplines. Early applica tions focused on the detection of microbial antigens in tissues, while more recent applications have used monoclonal antibodies to study cell differentiation during embryonic development. For a select few disci plines, volumes have been published focusing on the specific applica tion of immunocytochemical techniques to that discipline. What distinguishes the present book, Immunocytochemical Meth ods and Protocols, from earlier books is its broad appeal to researchers in all disciplines, including those in both research and clinical settings. The methods and protocols presented here are designed to be general in their application and the accompanying "Notes" provide invaluable assistance in adapting or troubleshooting the protocols. Interspersed throughout the book are chapters providing overviews of select topics related to immunocytochemistry.
Immunophenotyping
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Author : J Philip McCoy Jr
language : en
Publisher: Humana
Release Date : 2019-09-29
Immunophenotyping written by J Philip McCoy Jr and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-09-29 with Medical categories.
This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolving field.
B Cell Protocols
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Author : Hua Gu
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
B Cell Protocols written by Hua Gu and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
B-lymphocyte development and function remains an exciting area of research for those interested in the physiology and pathology of the immune system in higher animals. While recent advances in genetics and cellular and molecular biology have provided a large spectrum of powerful new experimental tools in this field, it is both time consuming and often very difficult for a student or just any bench-side worker to identify a reliable experimental protocol in the ocean of the literature. The aim of B Cell Protocols is to provide a collection of diverse protocols ranging from the latest inventions and applications to some classic, but still frequently used methods in B-cell biology. The authors of the various chapters are all highly qualified scientists who are either the inventors or expert users of these methods. Their extensive experience in mastering a particular method provides not only the step-by-step details of a reproducible protocol, but also useful troubleshooting tips that readers will appreciate in their daily work. We hope that this book will be helpful for both beginning and experienced researchers in the field in designing or modifying an experimental approach, and exploring a biological question from multiple angles.
Monoclonal Antibodies
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Author : Vincent Ossipow
language : en
Publisher: Humana
Release Date : 2016-08-23
Monoclonal Antibodies written by Vincent Ossipow and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Medical categories.
Monoclonal Antibodies: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed modern approaches to isolate and characterize monoclonal antibodies against carefully selected epitopes. This edition includes new chapters covering the key steps to generate high quality monoclonals via different methods, from antigen generation to epitope mapping and quality control of the purified IgG. Chapters are divided into four parts corresponding to four distinct objectives. Part I covers monoclonal antibody generation, Part II deals with monoclonal antibody expression and purification, Part III presents methods for monoclonal antibody characterization and modification, and Part IV describes selected applications of monoclonal antibodies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Monoclonal Antibodies: Methods and Protocols, Second Edition provides crucial initial steps of monoclonal antibody generation and characterization with state-of-the art protocols.