Single Molecule And Single Cell Sequencing

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Single Molecule And Single Cell Sequencing

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Author : Yutaka Suzuki
language : en
Publisher: Springer
Release Date : 2019-04-09

Single Molecule And Single Cell Sequencing written by Yutaka Suzuki and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-04-09 with Medical categories.

This book presents an overview of the recent technologies in single molecule and single cell sequencing. These sequencing technologies are revolutionizing the way of the genomic studies and the understanding of complex biological systems. The PacBio sequencer has enabled extremely long-read sequencing and the MinION sequencer has made the sequencing possible in developing countries. New developments and technologies are constantly emerging, which will further expand sequencing applications. In parallel, single cell sequencing technologies are rapidly becoming a popular platform. This volume presents not only an updated overview of these technologies, but also of the related developments in bioinformatics. Without powerful bioinformatics software, where rapid progress is taking place, these new technologies will not realize their full potential. All the contributors to this volume have been involved in the development of these technologies and software and have also made significant progress on their applications. This book is intended to be of interest to a wide audience ranging from genome researchers to basic molecular biologists and clinicians.

Transcriptome And Single Cell Sequencing Analyses To Classify Immune Subtypes Uncover Novel Biomarkers And Assess Immunotherapeutic Responses In Cancer

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Author : Hongda Liu
language : en
Publisher: Frontiers Media SA
Release Date : 2024-07-24

Transcriptome And Single Cell Sequencing Analyses To Classify Immune Subtypes Uncover Novel Biomarkers And Assess Immunotherapeutic Responses In Cancer written by Hongda Liu and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-07-24 with Medical categories.

According to the most recent projections of the International Agency for Research on Cancer (IARC), there would be around 19.3 million new cases of cancer and 10 million cancer-related deaths globally in 2022. Cancer research has never halted. In particular, research into the cancer immunological microenvironment is gaining popularity.

Single Molecule Analysis

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Author : Iddo Heller
language : en
Publisher: Springer Nature
Release Date : 2023-10-12

Single Molecule Analysis written by Iddo Heller and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-10-12 with Science categories.

This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who want to learn more about this exciting and still expanding field. Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Single Molecule Sequencing Of Rna

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Author : Dina Grohmann
language : en
Publisher: American Chemical Society
Release Date : 2025-04-24

Single Molecule Sequencing Of Rna written by Dina Grohmann and has been published by American Chemical Society this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-04-24 with Science categories.

What biological questions can single-molecule RNA sequencing help address that traditional methods cannot? How can these technologies be applied in your area of research, and what limitations might you encounter? Are specific bioinformatics tools or experimental protocols particularly suited to your needs? Single-Molecule Sequencing of RNA provides the reader with answers to these questions. Recent advances in RNA sequencing technologies have revolutionized the field of transcriptomics, shedding new light on the complexities of RNA biology. Single-molecule RNA sequencing, in particular, has opened unprecedented avenues for exploring gene expression, isoform diversity, RNA modifications, and dynamic transcript behaviors at single-molecule resolution. This transition from bulk RNA analyses to single-molecule studies represents a paradigm shift, enabling researchers to study the transcriptome with unparalleled accuracy and detail one molecule at a time. With this primer, the authors provide an understandable and comprehensive introduction to single-molecule RNA sequencing, beginning with a historical perspective and progressing to the latest technological developments. Key topics include principles of the currently most relevant third-generation single-molecule platforms from PacBio and Oxford Nanopore Technologies, developments on RNA library preparation, sequencing workflows, data analysis pipelines, and examples of real-world applications. Throughout the chapters, the authors emphasize both the opportunities and challenges presented by single-molecule RNA-seq, from its technical limitations to its unique capabilities in resolving full-length transcripts, detecting rare isoforms, and mapping epitranscriptomic modifications.

Advances In Single Molecule Real Time Smrt Sequencing

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Author : Adam Ameur
language : en
Publisher: MDPI
Release Date : 2019-11-18

Advances In Single Molecule Real Time Smrt Sequencing written by Adam Ameur and has been published by MDPI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-11-18 with Science categories.

PacBio’s single-molecule real-time (SMRT) sequencing technology offers important advantages over the short-read DNA sequencing technologies that currently dominate the market. This includes exceptionally long read lengths (20 kb or more), unparalleled consensus accuracy, and the ability to sequence native, non-amplified DNA molecules. From fungi to insects to humans, long reads are now used to create highly accurate reference genomes by de novo assembly of genomic DNA and to obtain a comprehensive view of transcriptomes through the sequencing of full-length cDNAs. Besides reducing biases, sequencing native DNA also permits the direct measurement of DNA base modifications. Therefore, SMRT sequencing has become an attractive technology in many fields, such as agriculture, basic science, and medical research. The boundaries of SMRT sequencing are continuously being pushed by developments in bioinformatics and sample preparation. This book contains a collection of articles showcasing the latest developments and the breadth of applications enabled by SMRT sequencing technology.

Single Cell Biomedicine

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Author : Jianqin Gu
language : en
Publisher: Springer
Release Date : 2018-06-25

Single Cell Biomedicine written by Jianqin Gu and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-06-25 with Science categories.

The book focuses on various detection targets applied in single cell studies, including tumor tissue cells, circulating tumor cells (CTCs), disseminated tumor cells (DTCs), circulating tumor DNA (ctDNA), cell-free DNA (cfDNA) and cancer stem cells (CSCs). It also discusses and compares detection methods using these detection targets in different fields to reveal single cell biomedical functions. The volume focuses not only on the methods already been established and validated, and also the methods newly developed. The book also highlights the importance and potential of single cell biomedicine in the development and validation of precision medicine strategies. It is useful for researchers and students in the field of cell biology, molecular medicine and precision medicine etc.

Recent Advances In Parasitomics

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Author : Juan David Ramírez González
language : en
Publisher: Springer Nature
Release Date : 2025-01-25

Recent Advances In Parasitomics written by Juan David Ramírez González and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-01-25 with Science categories.

This book presents a new era in parasitology research and involves several contributions on translational results. Readers are equipped with the recent advances of parasitomics (the OMICS study of parasites). World-renowned experts in the field of molecular biology and omics provide a glance of how omics have revolutionized our understanding of parasites and vectors, including the implications of these advances. A particular focus is on comprehending the biology, diagnostics, surveillance, response to treatment, prevention, and control of parasites. Final, cutting-edge studies on disease vectors round out this volume. Overall, five thematic book sections provide a clear overview and smooth findability of contents. Parasitic diseases represent an important burden for healthcare systems, particularly in endemic countries. In recent decades, there have been several advances in dissecting the genome, transcriptome and proteome of parasites allowing researchers worldwide to exploit this information and ameliorate our understanding of parasitoses and infections. For the first time, this collection will permit scientists, students, and all medical professionals around the world to update their knowledge and uncover the impact of going from basic science to implementation in order to mitigate parasitic diseases. In this way, the present work makes an indispensable contribution to practical progress regarding research on Neglected Tropical Diseases (NTDs) and the Sustainable Development Goal (SDG) 2, Good Health and Well-Being.

Information And Communication Theory In Molecular Biology

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Author : Martin Bossert
language : en
Publisher: Springer
Release Date : 2017-08-01

Information And Communication Theory In Molecular Biology written by Martin Bossert and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-08-01 with Technology & Engineering categories.

This edited monograph presents the collected interdisciplinary research results of the priority program “Information- and Communication Theory in Molecular Biology (InKoMBio, SPP 1395)”, funded by the German Research Foundation DFG, 2010 until 2016. The topical spectrum is very broad and comprises, but is not limited to, aspects such as microRNA as part of cell communication, information flow in mammalian signal transduction pathway, cell-cell communication, semiotic structures in biological systems, as well as application of methods from information theory in protein interaction analysis. The target audience primarily comprises research experts in the field of biological signal processing, but the book is also beneficial for graduate students alike.

Phylogenomics

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Author : Christoph Bleidorn
language : en
Publisher: Springer
Release Date : 2017-06-01

Phylogenomics written by Christoph Bleidorn and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-06-01 with Science categories.

This unique textbook provides a clear and concise overview of the key principles of the complex field of phylogenomics, with a particular focus on sequencing technologies that are crucial to studying and understanding interrelations in evolutionary genomics. It includes chapters dedicated to the analysis of nucleotide sequences using assembling and alignment methods and also discusses the main strategies for phylogenetic studies, systematic errors and their correction. This highly readable textbook is intended for graduate students and young researchers with an interest in phylogenetics and evolutionary developmental biology.

Advances In Soil Microbiology Recent Trends And Future Prospects

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Author : Tapan Kumar Adhya
language : en
Publisher: Springer
Release Date : 2018-02-28

Advances In Soil Microbiology Recent Trends And Future Prospects written by Tapan Kumar Adhya and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-02-28 with Science categories.

This book presents a comprehensive collection of articles illustrating the importance of microbial community structure and function for ecosystem sustainability and environmental reclamation. It addresses a diverse range of topics, including microbial diversity, physiology, genomics, ecosystem function, interaction, metabolism, and the fruitful use of microbial communities for crop productivity and environmental remediation. In addition, the book explores issues ranging from general concepts on the diversity of microorganisms in soil, and ecosystem function to the evolution and taxonomy of soil microbiota, with future prospects. It covers cutting-edge methods in soil microbial ecological studies, rhizosphere microflora, the role of organic matter in plant productivity, biological nitrogen fixation and its genetics, microbial transformation of plant nutrients in soil, plant-growth-promoting rhizobacteria, and organic matter transformation. The book also discusses the application of microbes in biodegradation of xenobiotic contaminants. It covers bio-fertilizers and their role in sustainable agriculture and soil health, biological control of insect pests and plant pathogens, and the latest tools of omics in soil microbiology, i.e. genomics, proteomics, transcriptomics and metabolomics, which offer pioneering approaches to the exploration of microbial structure and function.