Sted

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New Concepts For Sted Microscopy

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Author : Julia Kratz
language : en
Publisher:
Release Date : 2022

New Concepts For Sted Microscopy written by Julia Kratz and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022 with categories.

Fluorescence nanoscopy allows to non-invasively resolve three-dimensional cellular structures beyond the diffraction limit. One of these high resolution imaging techniques is stimulated emission depletion (STED) microscopy. However, the practically achievable resolution of a STED microscope is often limited by photobleaching. One method to overcome this limitation is tomographic STED (tomoSTED) microscopy. In tomoSTED microscopy, excited fluorophores in the sample plane are depleted by 1D STED patterns, which lead to an effective narrowing of the fluorescence-allowed area in a single direct...

Sted

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Author : Anne-Marie Mai
language : da
Publisher: Aarhus Universitetsforlag
Release Date : 2010-10-20

Sted written by Anne-Marie Mai and has been published by Aarhus Universitetsforlag this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-10-20 with Literary Criticism categories.

Redigeret af Dan Ringgaard og Anne-Marie MaiFornemmelsen af at leve i harmoni med et historisk forankret sted er med globaliseringen blevet udfordret, og stedet som sadan er kommet i fokus. Denne bog giver svar pa, hvad stedet kan betyde for os, og hvordan man analyserer stedets funktion og betydning i litteraturen.Sted introducerer til stedets teori og beskriver dens udvikling fra 1970'erne og frem. Studiet af stedet er udprAeget tvAerfagligt og sAetter begreber som landskab, rum og topografi i spil med fx kosmopolitisme, okologi, identitet, urbanisering og hjemstavn.Perspektivet spAender fra fAenomenologi og sociologi til litterAer kartografi og litteraturhistorie, og antologien forener centrale stedsteoretiske tekster med litterAere tematiseringer af stedet.

Biological Fluorescence Imaging On The Nanoscale

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Author : Lana Lau
language : en
Publisher:
Release Date : 2013

Biological Fluorescence Imaging On The Nanoscale written by Lana Lau and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with categories.

A majority of biological microscopy investigations involve the focusing of visible light with conventional lenses. Fluorescence microscopy is one of the most widely used tools in biology but its resolution has historically suffered from the diffraction limit to about 200 nm laterally and 800 nm axially. In the past decade, this resolution problem has been overcome by the rapidly emerging field of super-resolution microscopy. The first demonstrated super-resolution technique, STimulated Emission Depletion (STED) Microscopy, is the topic of this Dissertation. This Dissertation has two primary areas of focus: the design optimization of a STED microscope, covered in Chapters 2-4, and its application to super-resolution imaging in cells and tissues, covered in Chapters 4-6. Chapter 2 describes the STED apparatus and experimental methods used. This chapter covers the guiding principles behind the design of a STED microscope, which forms a basis for understanding the logic underlying the homebuilt STED microscope which was constructed for this research. This STED microscope has a typical resolution of approximately 60 nm (full-width-at-half-maximum) or 25 nm (sigma) and has the sensitivity to image single fluorophores. In Chapter 3, a framework for evaluating and optimizing STED performance in the presence of several key tradeoffs is presented. Chapter 4 describes both developments in STED Microscopy required to utilize far-red-emitting dyes and the challenges associated with performing super-resolution imaging in intact Drosophila tissue. In Chapter 5, the optimization of labeling density revealed the 9-fold symmetry of a centriole protein structure, an important organelle in cell development. In Chapter 6, Huntingtin protein aggregates are resolved beyond the diffraction limit in a cell model of the neurodegenerative Huntington's disease.

Automated Aberration Correction For Sted Microscopy

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Author : Debadrita Ghosh
language : en
Publisher:
Release Date : 2023

Automated Aberration Correction For Sted Microscopy written by Debadrita Ghosh and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023 with categories.

Stimulated emission depletion (STED) microscopy is a powerful super resolution fluorescence microscopy technique that surpasses the diffraction limit. However, the performance of a STED microscope can be adversely influenced by aberrations. In thick biological specimens, aberrations are primarily induced by wavefront distortion caused by variations in the refractive index within the specimen. These aberrations have a substantial impact on the quality of the acquired images and impose limitations on the achievable resolution. This problem can be resolved by using adaptive optics (AO) in a fe...

Sted Fluorescence Correlation Spectroscopy For Dynamic Observations In Cell Biology

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Author : Ruixing Wang
language : en
Publisher:
Release Date : 2018

Sted Fluorescence Correlation Spectroscopy For Dynamic Observations In Cell Biology written by Ruixing Wang and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with categories.

Super-resolution techniques offer new insight into the description of the dynamic molecular organization at the plasma membrane. Among these techniques, the stimulated emission depletion (STED) microscopy breaks the optical diffraction limit and reaches the resolution of tens of nanometer. It is a versatile setup that can be combined with other techniques such as fluorescence correlation spectroscopy (FCS), providing both high spatial and temporal resolutions to explore dynamic processes occurring in live cells. This PhD project aims at implementing a STED microscope, and then at combining this STED module with FCS technique for biological applications. Detailed theoretical studies on STED and the combined STED-FCS technique in spatio-temporal aspects were performed. An analytical solution for FCS autocorrelation function was derived in the condition of incomplete STED depletion and a new FCS fitting model was proposed to overcome this problem. The spot variation FCS (svFCS) method has demonstrated its capability to identify the presence of nanodomains constraining the lateral diffusion of molecules at the plasma membrane. The STED-FCS can extend the svFCS approach to the nanoscale evaluating the long-lasting existence of such nanodomains. Within this frame, preliminary Monte Carlo simulations were conducted mimicking molecules diffusing in the presence of dynamic self-assembling/disassembling nanodomains.

Nanoscale Photonic Imaging

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Author : Tim Salditt
language : en
Publisher: Springer Nature
Release Date : 2020-06-09

Nanoscale Photonic Imaging written by Tim Salditt and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-06-09 with Science categories.

This open access book, edited and authored by a team of world-leading researchers, provides a broad overview of advanced photonic methods for nanoscale visualization, as well as describing a range of fascinating in-depth studies. Introductory chapters cover the most relevant physics and basic methods that young researchers need to master in order to work effectively in the field of nanoscale photonic imaging, from physical first principles, to instrumentation, to mathematical foundations of imaging and data analysis. Subsequent chapters demonstrate how these cutting edge methods are applied to a variety of systems, including complex fluids and biomolecular systems, for visualizing their structure and dynamics, in space and on timescales extending over many orders of magnitude down to the femtosecond range. Progress in nanoscale photonic imaging in Göttingen has been the sum total of more than a decade of work by a wide range of scientists and mathematicians across disciplines, working together in a vibrant collaboration of a kind rarely matched. This volume presents the highlights of their research achievements and serves as a record of the unique and remarkable constellation of contributors, as well as looking ahead at the future prospects in this field. It will serve not only as a useful reference for experienced researchers but also as a valuable point of entry for newcomers.

Sted Microscopy With Two Photon Photoactivation In The Visible Range Application Of Generative Adversarial Networks For Image Reconstruction In Sted Microscopy

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Author : Jan-Erik Bredfeldt
language : en
Publisher:
Release Date : 2023

Sted Microscopy With Two Photon Photoactivation In The Visible Range Application Of Generative Adversarial Networks For Image Reconstruction In Sted Microscopy written by Jan-Erik Bredfeldt and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023 with categories.

The resolution limit of classical optical far-field microscopes was broken with the invention of super-resolution microscopy. Today, a variety of these techniques achieving different degrees of resolution exist. Image formation with most of these methods can be easily affected by either photobleaching of fluorescent dyes due to the use of high-intensity lasers or out-of-focus fluorescence signal from dyes in different imaging planes. The resulting loss in contrast reduces the possibility to distinguish individual structures or to localize individual fluorophores, ultimately limiting the gain in re...

Super Resolution Microscopy

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Author : Udo J. Birk
language : en
Publisher: John Wiley & Sons
Release Date : 2017-12-04

Super Resolution Microscopy written by Udo J. Birk and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-04 with Science categories.

This unique book on super-resolution microscopy techniques presents comparative, in-depth analyses of the strengths and weaknesses of the individual approaches. It was written for non-experts who need to understand the principles of super-resolution or who wish to use recently commercialized instruments as well as for professionals who plan to realize novel microscopic devices. Explaining the practical requirements in terms of hardware, software and sample preparation, the book offers a wealth of hands-on tips and practical tricks to get a setup running, provides invaluable help and support for successful data acquisition and specific advice in the context of data analysis and visualization. Furthermore, it addresses a wide array of transdisciplinary fields of applications. The author begins by outlining the joint efforts that have led to achieving super-resolution microscopy combining advances in single-molecule photo-physics, fluorophore design and fluorescent labeling, instrument design and software development. The following chapters depict and compare current main standard techniques such as structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy and multi-scale imaging including light-sheet and expansion microscopy. For each individual approach the experimental setups are introduced, the imaging protocols are provided and the various applications illustrated. The book concludes with a discussion of future challenges addressing issues of routine applications and further commercialization of the available methods. Guiding users in how to make choices for the design of their own experiments from scratch to promising application, this one-stop resource is intended for researchers in the applied sciences, from chemistry to biology and medicine to physics and engineering.

Fluorescence Microscopy

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Author : Ulrich Kubitscheck
language : en
Publisher: John Wiley & Sons
Release Date : 2017-03-27

Fluorescence Microscopy written by Ulrich Kubitscheck and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-03-27 with Medical categories.

While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.

Multiphoton Microscopy And Fluorescence Lifetime Imaging

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Author : Karsten König
language : en
Publisher: Walter de Gruyter GmbH & Co KG
Release Date : 2018-01-22

Multiphoton Microscopy And Fluorescence Lifetime Imaging written by Karsten König and has been published by Walter de Gruyter GmbH & Co KG this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-01-22 with Science categories.

This monograph demonstrates the latest developments in two-photon fluorescence microscopy and second-harmonic generation (SHG) microscopy, including coverage of high-resolution microscopy methods, such as STED microscopy. A special focus lies on clinical applications of these methods, e.g. in dermatology, ophtalmology, neuro sciences and cell biology.