Ensemble And Single Molecule Dynamics Of Intraflagellar Transport In C Elegans
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Ensemble And Single Molecule Dynamics Of Intraflagellar Transport In C Elegans
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Author :
language : en
Publisher:
Release Date : 2018
Ensemble And Single Molecule Dynamics Of Intraflagellar Transport In C Elegans written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with categories.
Handbook Of Dynein Second Edition
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Author : Keiko Hirose
language : en
Publisher: CRC Press
Release Date : 2019-05-28
Handbook Of Dynein Second Edition written by Keiko Hirose and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-05-28 with Science categories.
Dyneins are molecular motors that are involved in various cellular processes, such as cilia and flagella motility, vesicular transport, and mitosis. Since the first edition of this book was published in 2012, there has been a significant breakthrough: the crystal structures of the motor domains of cytoplasmic dynein have been solved and the previously unknown details of this huge and complex molecule have been unveiled. This new edition contains 14 chapters written by researchers in the US, Europe, and Asia, including 3 new chapters that incorporate new fields. The other chapters have also been substantially updated. Compared with the earlier edition, this book focuses more on the motile mechanisms of dynein, especially by biophysical methods such as cryo-EM, X-ray crystallography, and single-molecule nanometry. It is a major handbook for frontline researchers as well as for advanced students studying cell biology, molecular biology, biochemistry, biophysics, and structural biology.
Intraflagellar Transport In Caenorhabditis Elegans
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Author : Peter Nicholas Inglis
language : en
Publisher:
Release Date : 2009
Intraflagellar Transport In Caenorhabditis Elegans written by Peter Nicholas Inglis and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with Caenorhabditis elegans categories.
Intraflagellar transport (IFT) is the dynamic bidirectional process required for the biogenesis and maintenance of eukaryotic cilia. Landmark studies exploiting the model organism Chlamydomonas reinhardtii have provided a basic mechanism for the process, although recent research examining IFT in the nematode Caenorhabditis elegans has revealed a greater complexity to the original model of IFT described in Chlamydomonas, which includes the orthologues of several human proteins involved in cilium-associated diseases. The wealth of genomic, bioinformatic, and molecular tools available to C. elegans researchers is exploited in this thesis to uncover and characterise a number of novel proteins involved in the process of IFT, namely DYF-11, MKS-1, MKSR-1, and MKSR-2. DYF-11 localises throughout nematode ciliary structures and acts as a key component of the core IFT subcomplex B. The latter three proteins are members of a previously uncharacterised family of B9-domain containing polypeptides, all of which appear to localise at the base of cilia (basal body/transition zone), where they are found to regulate a cilium-based insulin signalling pathway. The results of this study contribute to the growing realisation in C. elegans cilia research that a network of transition-zone-specific proteins are participating in ciliary processes ranging from subtle modulation of ciliary signalling pathways to the actual biogenesis and maintenance of the organelle. Next, we examine previously isolated nematode strains that are defective in paraquat resistance for impaired retrograde IFT. We identify a retrograde-IFT deficient strain that likely represents a mutation in an IFT component that has not been previously characterised in C. elegans; the mutation maps closely to a highly conserved protein identified as a core component of IFT subcomplex A. Finally, we examine the role played by the IFT-associated Bardet-Biedl syndrome (BBS) proteins in C. elegans thermosensation. bbs mutant worms appear to be slightly defective in responding to both noxious and physiological temperatures. Furthermore, the nature of the physiological temperature defect correlates with a decreased roaming ability that is not the result of impaired locomotion. Altogether, the studies presented in this thesis offer novel insights into both the molecular makeup and physiological functions of IFT in C. elegans.
Single Molecule Analysis
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Author : Erwin J. G. Peterman
language : en
Publisher: Humana Press
Release Date : 2011-09-15
Single Molecule Analysis written by Erwin J. G. Peterman and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-09-15 with Science categories.
Life scientists believe that life is driven, directed, and shaped by biomolecules working on their own or in concert. It is only in the last few decades that technological breakthroughs in sensitive fluorescence microscopy and single-molecule manipulation techniques have made it possible to observe and manipulate single biomolecules and measure their individual properties. The methodologies presented in Single Molecule Techniques: Methods and Protocols are being applied more and more to the study of biologically relevant molecules, such as DNA, DNA-binding proteins, and motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. The aim of Single Molecule Techniques: Methods and Protocols is to provide a broad overview of single-molecule approaches applied to biomolecules on the basis of clear and concise protocols, including a solid introduction to the most widely used single-molecule techniques, such as optical tweezers, single-molecule fluorescence tools, atomic force microscopy, magnetic tweezers, and tethered particle motion. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Single Molecule Techniques: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and provides a broad and thorough overview of the exciting and still-emerging field of single-molecule biology.
Primary Cilia
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Author :
language : en
Publisher: Academic Press
Release Date : 2009-11-30
Primary Cilia written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-11-30 with Science categories.
In recent years, the role of cilia in the study of health, development and disease has been increasingly clear, and new discoveries have made this an exciting and important field of research. This comprehensive volume, a complement to the new three-volume treatment of cilia and flagella by King and Pazour, presents easy-to-follow protocols and detailed background information for researchers working with cilia and flagella. *Covers protocols for primary cilia across several systems and species * Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time * Relevant to clinicians and scientists working in a wide range of fields
Nature S Gift To Neuroscience
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Author : Chun-Fang Wu
language : en
Publisher: CRC Press
Release Date : 2022-02-27
Nature S Gift To Neuroscience written by Chun-Fang Wu and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-02-27 with Science categories.
In the 1960s, Sydney Brenner proposed to use the nematode worm Caenorhabditis elegans to discover the control mechanisms of animal development and to reveal how a small number of neurons generate different behaviours, giving birth to a vibrant community that uses this animal model for their studies. Brenner was aided in his aim by John Sulston, who mapped the C. elegans cell lineages – from a single cell to the multicellular adult – which transformed the field of developmental biology. As a tribute to these two men, this book captures the perspectives of some of the early pioneers of the worm community, from Martin Chalfie, Robert Waterston and Donald Moerman to Catherine Rankin, Antony Stretton and John White. It also includes contributions from subsequent generations of the community, who explore the development and function of the C. elegans nervous system. This book features how this animal has become one of the best models for elucidating the biology of different sensory modalities and their complex behavioural outputs, or how this animal’s survival strategies have contributed to our understanding of ageing and neurodegeneration. Thus, this volume documents the development of the C. elegans neuroscience field, from infancy to maturity. The chapters in this book were originally published as a special issue of the Journal of Neurogenetics.
Microtubule Dynamics
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Author : Anne Straube
language : en
Publisher: Humana Press
Release Date : 2017-04-30
Microtubule Dynamics written by Anne Straube and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-04-30 with Science categories.
Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and back. In Microtubule Dynamics: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics in vitro and in cells. Beginning with the question of how to analyze microtubule dynamics, the volume continues with detailed descriptions of how to isolate tubulin from different sources and with different posttranslational modifications, methods used to study microtubule dynamics and microtubule interactions in vitro, techniques to investigate the ultrastructure of microtubules and associated proteins, assays to study microtubule nucleation, turnover, and force production in cells, as well as approaches to isolate novel microtubule-associated proteins and their interacting proteins. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Definitive and practical, Microtubule Dynamics: Methods and Protocols provides the key protocols needed by novices and experts on how to perform a broad range of well-established and newly-emerging techniques in this vital field.
E Coli In Motion
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Author : Howard C. Berg
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-01-11
E Coli In Motion written by Howard C. Berg and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-01-11 with Science categories.
Escherichia coli, commonly referred to as E. coli, has been the organism of choice for molecular genetics for decades. Its machinery and mobile behavior is one of the most fascinating topics for cell scientists. Scientists and engineers, not trained in microbiology, and who would like to learn more about living machines, can see it as a unique example. This cross-disciplinary monograph covers more than thirty years of research and is accessible to graduate students and scientists alike.
Stochastic Processes In Cell Biology
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Author : Paul C. Bressloff
language : en
Publisher: Springer Nature
Release Date : 2022-01-04
Stochastic Processes In Cell Biology written by Paul C. Bressloff and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-01-04 with Mathematics categories.
This book develops the theory of continuous and discrete stochastic processes within the context of cell biology. In the second edition the material has been significantly expanded, particularly within the context of nonequilibrium and self-organizing systems. Given the amount of additional material, the book has been divided into two volumes, with volume I mainly covering molecular processes and volume II focusing on cellular processes. A wide range of biological topics are covered in the new edition, including stochastic ion channels and excitable systems, molecular motors, stochastic gene networks, genetic switches and oscillators, epigenetics, normal and anomalous diffusion in complex cellular environments, stochastically-gated diffusion, active intracellular transport, signal transduction, cell sensing, bacterial chemotaxis, intracellular pattern formation, cell polarization, cell mechanics, biological polymers and membranes, nuclear structure and dynamics, biological condensates, molecular aggregation and nucleation, cellular length control, cell mitosis, cell motility, cell adhesion, cytoneme-based morphogenesis, bacterial growth, and quorum sensing. The book also provides a pedagogical introduction to the theory of stochastic and nonequilibrium processes – Fokker Planck equations, stochastic differential equations, stochastic calculus, master equations and jump Markov processes, birth-death processes, Poisson processes, first passage time problems, stochastic hybrid systems, queuing and renewal theory, narrow capture and escape, extreme statistics, search processes and stochastic resetting, exclusion processes, WKB methods, large deviation theory, path integrals, martingales and branching processes, numerical methods, linear response theory, phase separation, fluctuation-dissipation theorems, age-structured models, and statistical field theory. This text is primarily aimed at graduate students and researchers working in mathematical biology, statistical and biological physicists, and applied mathematicians interested in stochastic modeling. Applied probabilists should also find it of interest. It provides significant background material in applied mathematics and statistical physics, and introduces concepts in stochastic and nonequilibrium processes via motivating biological applications. The book is highly illustrated and contains a large number of examples and exercises that further develop the models and ideas in the body of the text. It is based on a course that the author has taught at the University of Utah for many years.
Microinjection And Transgenesis
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Author : Angel Cid-Arregui
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Microinjection And Transgenesis written by Angel Cid-Arregui and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The establishment of microinjection protocols about 20 years ago for cultured cells and shortly thereafter for the generation of transgenic mice by microinjection of DNA into fertilized mouse eggs greatly influ enced many fields of biology. Not only have the data generated using these approaches contributed to a large extent to our present under standing of gene regulation and cellular function of higher eukaryotic cells, but current knowledge and future developments in this area will certainly have a great impact on basic and applied research for many years to come. This laboratory manual describes the current state of the art in this research area and focuses primarily on both the experimental strategies with an extensive bibliography and the detailed procedures. A large number of studies are presently being performed and a great variety of new experimental designs are rapidly being developed. The book con tains protocols on injection of somatic cells as well as on injection of embryos, the use of similar equipment being a common feature. In the articles dedicated to somatic cells, full descriptions of the manual and automatic injection systems are given as well as the methods for the analysis of injected cells by video-microscopy, electron microscopy or in situ hybridizations. In addition, comprehensive protocols are given for injection experiments with very different purposes, such as to study sig nal transduction or microtubule dynamics.