Microscopy Optical Spectroscopy And Macroscopic Techniques

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Microscopy Optical Spectroscopy And Macroscopic Techniques

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Author : Christopher Jones
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02

Microscopy Optical Spectroscopy And Macroscopic Techniques written by Christopher Jones and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.

This is the second of three volumes of Methods in Molecular Biology that deal with Physical Methods of Analysis. The first of these, Spectroscopic Methods and Analyses dealt with NMR spec troscopy, mass spectrometry, and metalloprotein techniques, and the third will cover X-ray crystallographic methods. As with the first volume. Microscopy, Optical Spectroscopy, and Macroscopic Techniques is intended to provide a basic understand ing for the biochemist or biologist who needs to collaborate with spe cialists in applying the techniques of modern physical chemistry to biological macromolecules. The methods treated in this book fall into four groups. Part One covers microscopy, which aims to visualize individual molecules or complexes of several molecules. Electron microscopy is the more familiar of these, while scanning tunneling microscopy is a new and rapidly developing tool. Methods for determining the shapes and sizes of molecules in solution are described in Part Two, which includes chapters on X-ray and neutron scattering, light scattering, and ult- centrifugation. Calorimetry, described in Part Three, provides the means to monitor processes involving thermodynamic changes, whether these are intramolecular, such as conformational transition, or the interactions between solutes or between a solute and its sol vent. Part Four is concerned with optical and infrared spectroscopy and describes applications ranging from the measurement of protein concentration by UV absorbance to the analysis of secondary struc ture using circular dichroism and Fourier-transform infrared spec troscopy.

Optical Spectroscopic And Microscopic Techniques

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Author : Harekrushna Sahoo
language : en
Publisher: Springer Nature
Release Date : 2022-02-25

Optical Spectroscopic And Microscopic Techniques written by Harekrushna Sahoo and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-02-25 with Science categories.

This book illustrates the significance of various optical spectroscopy and microscopy techniques, including absorption spectroscopy, fluorescence spectroscopy, infrared spectroscopy, and Raman spectroscopy for deciphering the nature of biological molecules. The content of this book chiefly focuses on (1) the principle, theory, and instrumentation used in different optical spectroscopy techniques, and (2) the application of these techniques in exploring the nature of different biomolecules (e.g., proteins, nucleic acids, enzymes, and carbohydrates). It emphasizes the structural, conformational and dynamic, and kinetic including the changes in biomolecules under a range of conditions. In closing, the book summarizes recent advances in the field of optical spectroscopic and microscopic techniques.

Optical Microscopic And Spectroscopic Techniques Targeting Biological Applications

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Author : Vicente Micó
language : en
Publisher: Frontiers Media SA
Release Date : 2021-10-22

Optical Microscopic And Spectroscopic Techniques Targeting Biological Applications written by Vicente Micó and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-10-22 with Science categories.

Methods In Plant Electron Microscopy And Cytochemistry

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Author : William V. Dashek
language : en
Publisher: Springer Science & Business Media
Release Date : 2000-06-29

Methods In Plant Electron Microscopy And Cytochemistry written by William V. Dashek and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-06-29 with Science categories.

Hands-on experimentalists describe the cutting-edge microscopical methods needed for the effective study of plant cell biology today. These powerful techniques, all described in great detail to ensure successful experimental results, range from light microscope cytochemistry, autoradiography, and immunocytochemistry, to recent developments in fluorescence, confocal, and dark-field microscopies. Important advances in both conventional and scanning electron microscopies are also fully developed, together with such state-of-the-art ancillary techniques as high-resolution autoradiography, immunoelectron microscopy, X-ray microanalysis, and electron systems imaging. Easy-to-use and up-to-date, Methods in Plant Electron Microscopy and Cytochemistry offers today's plant scientists a first class collection of readily reproducible light and electron microscopical methods that will prove the new standard for all working in the field.

Methods In Molecular Biology Microscopy Optical Spectroscopy And Macroscopic Techniques

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Author : John M. Walker
language : en
Publisher:
Release Date : 1984

Methods In Molecular Biology Microscopy Optical Spectroscopy And Macroscopic Techniques written by John M. Walker and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols categories.

Materials Characterization

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Author : Yang Leng
language : en
Publisher: John Wiley & Sons
Release Date : 2008-06-02

Materials Characterization written by Yang Leng and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-06-02 with Science categories.

Part One - Microstructure Examinations Light microscopy X-ray diffraction Transmission electron microscopy Scanning electron microscopy Scanning probe microscopy Part Two--Chemical and Thermal Analysis X-Ray Spectroscopy for Elemental Analysis Electron Spectroscopy for Surface Analysis Secondary Ion Mass Spectrometry for Surface Analysis Vibrational Spectroscopy for Molecular Analysis Thermal analysis.

Spectroscopic And Microscopic Techniques In Atmospheric Sciences

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Author : Lin Du
language : en
Publisher: John Wiley & Sons
Release Date : 2025-09-29

Spectroscopic And Microscopic Techniques In Atmospheric Sciences written by Lin Du and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-09-29 with Science categories.

A timely and current discussion of instrumental techniques in atmospheric sciences In Spectroscopic and Microscopic Techniques in Atmospheric Sciences, a team of distinguished researchers delivers an authoritative and up-to-date discussion of the latest developments in sophisticated analytical techniques and their applications in atmospheric sciences, including chemical identification and characterization, determination of reaction mechanisms and kinetics, and qualitative and quantitative analysis. Each chapter presents the development of a different analytical method from first principles and highlights the major developments in the technique to date. The book also covers the weaknesses and strengths of each technique and the suitability of its applications in indicated environmental media. Readers will also find: A thorough introduction to relevant atmospheric processes Comprehensive explorations of mass spectrometry and optical, electron, and atomic force microscopy Practical discussions of fluorescence spectroscopy and ultraviolet-visible absorption spectroscopy Complete treatments of infrared spectroscopy and Raman spectroscopy Perfect for graduate students undertaking courses in atmospheric sciences, Spectroscopic and Microscopic Techniques in Atmospheric Sciences will also benefit environmental chemists, climatologists, and analytical chemists.

Crc Handbook Of Viruses

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Author : Horace M. Mazzone
language : en
Publisher: CRC Press
Release Date : 1998-05-13

Crc Handbook Of Viruses written by Horace M. Mazzone and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-05-13 with Science categories.

As a distinct class of macromolecules, viruses are continually being studied in order to determine their properties. Following a knowledge of host-range infectivity, the particle mass-molecular weight of the virus and related properties - including size, shape, sedimentation, and diffusion coefficients - are also important characterizations. In the literature, these values have been determined for many viruses, and a variety of techniques are available by which such properties may be analyzed. Until now, there has been no single source for such information that the interested investigator may consult, and no databases provided this kind of information. CRC Handbook of Viruses: Mass-Molecular Weight Values and Related Properties corrects this deficiency by presenting such data for all classes of viruses; centering on viruses, their molecular weight, and their related properties; and acquainting the investigator to many methods for obtaining the mass-molecular weight value of viruses. This singular study explains the variety of methods available to the researcher as well as provides examples of each method. Molecular weight values are accessed directly from the book, saving the investigator a tedious search through the range of literature. Chapters discuss: Viruses as infectious agents and their role in establishing the relatively new discipline of molecular biology Essential components of viruses, protein, and nucleic acid - considering their discovery, nature, structural organization, and the forming of viruses from nucleoproteins Basic aspects of virus purification, preparative centrifuge, and various purification methods Basic aspects of crystallography, including procedures, x-ray analysis of the viral protein component, the arranging of subunits, and the composition of the intact virus Sedimentation experiments and studies used to obtain molecular weight Sedimentation and diffusion coefficients integral to the basic Svedberg equation Sedimentation equilibrium procedures The new Beckman Optima series of analytical ultracentrifuges Scattering studies, including small angle x-ray, small angle neutron, classical light scattering, and electron microscopy The renaissance of instrumentation in classical light scattering techniques Cold neutron facilities currently being set into operation by government laboratories Sizing and solvation of viruses in solution - their "natural environment" Critical modeling experiments Facilities and instrumentation for molecular weight studies - including the high voltage transmission electron microscope for obtaining mass values of viral inclusion bodies CRC Handbook of Viruses serves the: Researcher seeking values of virus molecular weight and related parameters Investigator getting started in virology and seeking information on physical chemical procedures Student interested in viruses as infectious agents

Protein Targeting Protocols

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Author : Roger A. Clegg
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04

Protein Targeting Protocols written by Roger A. Clegg and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.

It is by no means a revelation that proteins are not uniformly distributed throughout the cell. As a result, the idea that protein molecules, because of the specificity with which they can engage in interactions with other proteins, may be aimed—via these interactions—at a restricted target, is a fundamental one in contemporary molecular life sciences. The target may be variously c- ceived as a specific molecule, a group of molecules, a structure, or a more generic type of intracellular environment. Because the concept of protein targeting is intuitive rather than expl- itly defined, it has been variously used by different groups of researchers in cell biology, biochemistry, and molecular biology. For those working in the field of intracellular signaling, an influential introduction to the topic was the seminal article by Hubbard & Cohen (TIBS [1993] 18, 172–177), which was based on the work of Cohen’s laboratory on protein phosphatases. Sub- quently, the ideas that they discussed have been further developed and extended by many workers to other key intermediaries in intracellular sign- ing, including protein kinases and a great variety of modulator and adaptor proteins.

Methods In Protein Structure And Stability Analysis Vibrational Spectroscopy

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Author : Vladimir N. Uversky
language : en
Publisher: Nova Publishers
Release Date : 2007

Methods In Protein Structure And Stability Analysis Vibrational Spectroscopy written by Vladimir N. Uversky and has been published by Nova Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Science categories.

Protein research is a frontier field in science. Proteins are widely distributed in plants and animals and are the principal constituents of the protoplasm of all cells, and consist essentially of combinations of a-amino acids in peptide linkages. Twenty different amino acids are commonly found in proteins, and serve as enzymes, structural elements, hormones, immunoglobulins, etc., and are involved throughout the body, and in photosynthesis. This book gathers new leading-edge research from throughout the world in this exciting and exploding field of research.