Single Molecule Tools


Single Molecule Tools
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Single Molecule Tools Part A Fluorescence Based Approaches


Single Molecule Tools Part A Fluorescence Based Approaches
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Author :
language : en
Publisher: Academic Press
Release Date : 2010-08-17

Single Molecule Tools Part A Fluorescence Based Approaches written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-08-17 with Science categories.


Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part A: Fluorescence Based Approaches captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools Methods included are useful to both established researchers and newcomers to the field Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines



Single Molecule Tools Part B Super Resolution Particle Tracking Multiparameter And Force Based Methods


Single Molecule Tools Part B Super Resolution Particle Tracking Multiparameter And Force Based Methods
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Author :
language : en
Publisher: Academic Press
Release Date : 2010-07-09

Single Molecule Tools Part B Super Resolution Particle Tracking Multiparameter And Force Based Methods written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-07-09 with Science categories.


Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools Methods included are useful to both established researchers and newcomers to the field Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines



Methods In Enzymology


Methods In Enzymology
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Author : Nils G. Walter
language : en
Publisher:
Release Date : 2010

Methods In Enzymology written by Nils G. Walter and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with Enzymology categories.




Single Molecule Analysis


Single Molecule Analysis
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Author : Iddo Heller
language : en
Publisher: Springer Nature
Release Date : 2023-11-13

Single Molecule Analysis written by Iddo Heller and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-11-13 with Science categories.


This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who want to learn more about this exciting and still expanding field. Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.



Single Molecule Tools


Single Molecule Tools
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Author :
language : en
Publisher:
Release Date : 2010

Single Molecule Tools written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with categories.




Single Molecule Tools For Bioanalysis


Single Molecule Tools For Bioanalysis
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Author : Shuo Huang
language : en
Publisher: CRC Press
Release Date : 2022-05-26

Single Molecule Tools For Bioanalysis written by Shuo Huang and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-05-26 with Medical categories.


In the last three decades, the fast development of single-molecule techniques has revolutionized the way we observe and understand biological processes. Some of these techniques have been further adapted as tools for bioanalysis. This book summarizes and details the frontiers of the development of these tools as well as their applications. The contributors are young and established researchers in their respective fields. The main content originates from the lecture notes of a chemistry graduate course taught by the book editor at Nanjing University. This book is suitable to be used as a textbook for a high-level undergraduate or an entry-level graduate course. The systematically written content provides a thorough illustration of the mechanisms of each methodology presented.



Single Molecule Tools


Single Molecule Tools
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Author :
language : en
Publisher:
Release Date : 2010

Single Molecule Tools written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with Enzymology categories.




Single Molecule Tools


Single Molecule Tools
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Author : Nils G. Walter
language : en
Publisher:
Release Date : 2010

Single Molecule Tools written by Nils G. Walter and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with Biosensors categories.




Single Molecule Detection In Solution


Single Molecule Detection In Solution
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Author : Christoph Zander
language : en
Publisher: Wiley-VCH
Release Date : 2002-05-06

Single Molecule Detection In Solution written by Christoph Zander and has been published by Wiley-VCH this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-05-06 with Science categories.


The detection of single molecules opens up new horizons in analytical chemistry, biology and medicine. This discipline, which belongs to the expanding field of nanoscience, has been rapidly emerging over the last ten years. This handbook provides a thorough overview of the field. It begins with basics of single molecule detection in solution, describes methods and devices (fluorescense correlation spectroscopy, surface enhanced Raman scattering, sensors, especially dyes, screening techniques, especially confocal laser scanning microscopy). In the second part, various applications in life sciences and medicine provide the latest research results. This modern handbook is a highly accessible reference for a broad community from advanced researchers, specialists and company professionals in physics, spectroscopy, biotechnology, analytical chemistry, and medicine. Written by leading authorities in the field, it is timely and fills a gap - up to now there exists no handbook concerning this theme.



Single Molecule Biology


Single Molecule Biology
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Author : Alexander E. Knight
language : en
Publisher: Academic Press
Release Date : 2009-02-26

Single Molecule Biology written by Alexander E. Knight and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-02-26 with Science categories.


Single molecule techniques, including single molecule fluorescence, optical tweezers, and scanning probe microscopy, allow for the manipulation and measurement of single biological molecules within a live cell or in culture. These approaches, amongst the most exciting tools available in biology today, offer powerful new ways to elucidate biological function, both in terms of revealing mechanisms of action on a molecular level as well as tracking the behaviour of molecules in living cells. This book provides the first complete and authoritative treatment of this rapidly emerging field, explicitly from a biological perspective.The contents are organized by biological system or molecule. Each chapter discusses insights that have been revealed about their mechanism, structure or function by single molecule techniques. Among the topics covered are enzymes, motor proteins, membrane channels, DNA, ribozymes, cytoskeletal proteins, and other key molecules of current interest. An introduction by the editor provides a concise review of key principles and an historical overview. The last section discusses applications in molecular diagnostics and drug discovery. Organized by biological system or molecule Each chapter discusses insights into mechanism of action, structure, and function Covers enzymes, motor proteins, membrane channels, DNA, ribozymes, etc Includes an introduction to key principles and an historical overview Discusses applications in molecular diagnostics and drug discovery Provides an expert's perspective on future development